FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_27.3410000000a767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_27.3410000000a767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577388
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC31620.200457972293437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4500.036.49889445
GCCCACG4800.033.74897818
CCGAGCC5000.032.84900314
TATGCCG5300.031.4141443
CCCACGA5300.030.98962619
CGAGCCC5350.030.70000515
ATGCCGT5350.030.70000544
CGTATGC5500.029.86273441
AGCCCAC5450.029.72387117
CCACGAG5700.028.4201920
GTATGCC5900.027.8381442
CGAGACT5800.027.54226723
GAGCCCA6200.026.85402516
TCCGAGC6250.025.91921613
CTCCGAG6400.025.31173312
TATCTCG6600.024.54471236
ACGAGAC6700.024.51418122
CTCGTAT6850.023.97737739
CACGAGA6850.023.6489221
TCGTATG6950.023.63237840
Produced by FastQC (version 0.11.2)