FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_26.3410000000a75b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_26.3410000000a75b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848995
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC22790.2684350320084335TruSeq Adapter, Index 12 (95% over 21bp)
GTACTTAAGAAGATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAA11160.13144953739421317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2700.038.3322318
GTATGCC2900.035.6886342
CCCACGA2950.035.08373619
CGTATGC3000.034.49900441
TATGCCG3050.033.9334543
ATGCCGT3050.033.9334544
CCGAGCC3250.031.84523414
AGCCCAC3450.029.99913817
CGAGCCC3450.029.99913815
TCGTATG3550.029.15408940
CCACGAG3550.029.15408920
CGAGACT3450.028.69482823
GAGCCCA3650.028.35534916
CACGAGA3700.027.97216821
TGCCGTC3900.027.11460345
GTGACGT507.157636E-526.99922427
TCTCGTA3950.026.7713838
GTCCAGG608.108034E-626.28331
GACTACT3800.026.05188226
CTACTTG4000.025.87425628
Produced by FastQC (version 0.11.2)