FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_25.3410000000a731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_25.3410000000a731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445681
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC8890.19947002452426738RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1150.033.2596341
CCGGTCC300.005144857329.99887831
CTCGTAT1300.029.42197439
TCGTATG1300.029.42197440
TATGCCG1350.028.33227343
GTATGCC1350.028.33227342
CCCCTAC406.1011076E-428.1713581
ATTACAC654.863705E-727.6912693
CGAATTG1600.026.7177528
AGCCCAC1500.025.49904617
CCACGCA551.3667143E-424.54453714
GCCCACG1600.023.90535518
CGAGCCC1600.023.90535515
CGAGACG1600.023.90535523
GTATTAA851.8584251E-723.8627991
GTATCAA9250.023.3898431
GCTATCT1600.022.49915934
CGACTAA500.002262943222.49915933
ATGCCGT1700.022.49915944
TCCGAGC1750.021.85632513
Produced by FastQC (version 0.11.2)