FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_24.3410000000a724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_24.3410000000a724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTTACACTGGTGAAGATGATTACATACTTGAACTGTTCTTGCCTGTAAG10.8620689655172413No Hit
TGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCACA10.8620689655172413No Hit
CTGTTGGATCGGTGAAGCTTTTCGATGACACTAGGCTTGTTCTCCTCATCT10.8620689655172413No Hit
GCTCAAATCTGGACCTGCTGAAAAACCAATGATCGTCGTTAACTACAAGGG10.8620689655172413No Hit
TATCAACGCAGAGTACGGGAGGGTTTCTGTTTCGCCGCTCAGGTTTCGTGA10.8620689655172413No Hit
CCTTATTTTCAATTGGAAGAAAATTTCATCCACTTTTGATCAGATATACTG10.8620689655172413No Hit
ATCCAAAGGGATCGCATTGTCTCCAGCTTTGAAGCATTGGCCAAAAGCGCC10.8620689655172413No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGACTGTCTCT10.8620689655172413No Hit
CGACCCGTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAAC10.8620689655172413No Hit
ATGTCGGAGACAGCGGGGCAGTCGTTACACCATTCGTGCAGGTCGCTACTT10.8620689655172413No Hit
CCCACTTCACCATACGGCTCCCCATCAAGTCTCAGACTCCACACCAAAGTG10.8620689655172413No Hit
ATATTTCTCCATTTGTCCTTGAGATCCACATTAGAACGAGACTTCAGAATT10.8620689655172413No Hit
TGTTTGATAGAAAAAAAATGAATGCTTGCAACTGTGAAGACTGAAAAGTCA10.8620689655172413No Hit
CTTGTGGTCTCAACCTCCTGCTCTCTTCTCCACACCGCTGTTGATCTTATC10.8620689655172413No Hit
ATTCATATGCAACCAGGTCTAGTCATATAATTTGGCATGACCAATCAACTA10.8620689655172413No Hit
GTTCTACATCGTCTTCATGCTCACTGCTTGTGCTCTTTTCTCAAAGACATG10.8620689655172413No Hit
TTTCCCGGTTGTTCCACCTATGTTGATGGCATTGACAAAGAAGGCAAAAGG10.8620689655172413No Hit
GGAATACCAATACAGCAGTCGACAACAGCTGTATTCAAATTCTTCTCGGCA10.8620689655172413No Hit
GTGTGAACAATATAGGCATGGCCGAGCTTCTTAGGAATGAACGGCTGGGTT10.8620689655172413No Hit
GCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGGCTGTCTCTTAACCAT10.8620689655172413No Hit
NGTGTGATGAGAGCTAAGTGGAAGAAGAAGCGTATAAGGAGATTGAAGAGG10.8620689655172413No Hit
TCTACACACTTCTTAGTCTTGGAAAAAGCTTTAAGTTCATCAGCAAGACAG10.8620689655172413No Hit
TCTCAGGGAGGTGATCAAAGTCCACTGGGTTCTTTCTGGAGGAGCTTTGGA10.8620689655172413No Hit
ACCATCTTTAGTCAAAAGACATGAGGAAACATATGGTACCATCAGAAAAAA10.8620689655172413No Hit
TTATTACATATAAGCAAACCTAACATAACATTAGGGTTATCACAGACTTAG10.8620689655172413No Hit
TGCCAAGAAGGGTTAGAGTGGATGCAATATTGAAGATGATGAAGTTGCTCC10.8620689655172413No Hit
GTCCTCCAAGGTTGAAATGCTACCTGAAGATTTGAAATCTTCATCAGGTCT10.8620689655172413No Hit
GTCCACTTATAGGAACAGAAATGCAGAAACCACCGAGATACAAAACTCGAG10.8620689655172413No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8620689655172413No Hit
GGAGATAAGGAGGCGGCTCCTCCTGCTCCTGCTCCGGCAGCTCCAAAAGCT10.8620689655172413No Hit
CTCTCTTCCTCTTCTTCAACACCGACTCTGGAACTACAACCTTTGACTCAA10.8620689655172413No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA10.8620689655172413No Hit
GCTAACACCAAGTTTCCACCTCTCAGCTATAAGTCGAACCGTGTCTCGTTT10.8620689655172413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTCGTTTATACCCCTG10.8620689655172413No Hit
CTTGAAGAAGAGAGGCCGAGGAAAAAGGAAGGGATTTGAGATGACGACGAT10.8620689655172413No Hit
GGTGATAGCCCTGTAGATTCGTTCCTATGGTTCGATCCTTCCCAGTAAAAC10.8620689655172413No Hit
CTTATACACAGCTCTATGAAGCAATGGAGGCTTTTGTTCATATATGTAGAG10.8620689655172413No Hit
TTCCTAAGCTTCTCGAATTATGTTTTGTTAGAATATCGGCTGTCTCTTAAC10.8620689655172413No Hit
GACTACACCAATCTATGTCAGTTAGTGATTTCGTTGCATATTTATCTGATT10.8620689655172413No Hit
GATTATATAGTTTTGTGGTGTCCAGCAGGATTTAGTCTTACTGCAGTGCAA10.8620689655172413No Hit
ACCGAAGATATCGTAACTTATGAACTGTCCTCCGTGAGTTTCCACCGCCGG10.8620689655172413No Hit
ATCGTGTTGGATCTTCGGTGTTGGTTTTGCAAAACCCTAGAGCCCGTACTC10.8620689655172413No Hit
ACCTGATACTGTATAAGAGCTTGAAAACCAGCAGACTTCTGGAAAGTGACG10.8620689655172413No Hit
CCTTGAGGGCTCTTATAAGATCTTCTCTTGAATGAGATGCGGAGATGCAAA10.8620689655172413No Hit
CACTTCTGGATGTCATGGGATCCATCGACTAGGATCTCAGGCTCGACATTT10.8620689655172413No Hit
CTTAAACTCAGCGGGTAATCCCGCCTGACCTGGGGTCGCCCCGTACTCTGC10.8620689655172413No Hit
CAAAAGAAGACGACAGAATCCATAGCTACAAAGTACCATCAGATGATTGTT10.8620689655172413No Hit
TTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACG10.8620689655172413No Hit
CTAATGACCAAAACGTTATCATCGGGGAGTATGTTGTAGGTTCCCTCCACT10.8620689655172413No Hit
AACAATCTCTATCCTTCTCTCGATATGAATGATCTCGCTCGTAATCTCTTC10.8620689655172413No Hit
GATCTCGCTCTTTCCAAAATCCAAAAGACAACAAAAGATGATAAAACTCCA10.8620689655172413No Hit
GCTTGATCGAGTGGTGGTCCAATAGATTGGCCTTCTTAACATTCTTGTTGG10.8620689655172413No Hit
GTCTCAACACGTCCCACTGGCACCGTTCCAATACCACCAATCTTGTAGACA10.8620689655172413No Hit
TTCGCAGATCTGTCGATCGACCATGGGGATTGAACAAGATCTGTCTCTTAT10.8620689655172413No Hit
CATTCAACGTTTGGTACCTCACATTGGCGCTCACTCCCATATACGTTGCCT10.8620689655172413No Hit
ATATTGAGCAGGCGACGAACAAATTTCTTGATTACAAGCATAATAAAAACA10.8620689655172413No Hit
CGTCTAAGCCTTCCACGACCTGCCCAAACACCACGTGCTTCCCATCAAGCC10.8620689655172413No Hit
GTACAAATATTCCTCCAAATTCACAATTGAGGGTGATGTTTGCCTAGAATC10.8620689655172413No Hit
CCTTTCAGAGTGACCAAGGATAACCCATGGAATATCCAAGTTCACAAGCAT10.8620689655172413No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGC10.8620689655172413No Hit
CTGCTATGGAATTTCTAAATAGAATCTATATATATGAAGATTATGAACTAA10.8620689655172413No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGGC10.8620689655172413No Hit
TTCTTGGACTTATACCTGAGACTGGAAAGTTTGTTTCGGAGAGCGTCGAAG10.8620689655172413No Hit
GCATATTCTGATGTCAAACCAAATGGTGTGGCATATTCATGATGCACACTA10.8620689655172413No Hit
TGCTTATACCAAATCTACTGGAGATTTGACTCTCTCTGAGACACCAGAGTG10.8620689655172413No Hit
AGTCTGTTGAACCAAGTAATAGTTCAGGTGGACGATACCACAAAGTTACAA10.8620689655172413No Hit
AAATAAAAGAGAAAAAAGAAAAAGGCTCAAACGCCATCAAAGTTTTAAGAA10.8620689655172413No Hit
ATCTAGTGATGACCACCGGGAAGCTTCTCTTTAATCTTATCCATCGTACTT10.8620689655172413No Hit
ATGTGCATTTTGAACCTTTGTGACGGCGTTTTTAAAGAACCCCAAAACTGA10.8620689655172413No Hit
ATGGATCTCCGATCGGGAAACCGTATCCAAGCTCCGTGGCCTGTCTCTTAT10.8620689655172413No Hit
CCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGG10.8620689655172413No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAAT10.8620689655172413No Hit
GGACCAAGGTTCGAGTTGTAAAGGAGACGACATTTTTAGAGGCTTGCATTG10.8620689655172413No Hit
ATTCTACTGAAGAAACTATTGTAGTTAGTAACACTGTCCTAGAGCTGTCTC10.8620689655172413No Hit
TTCCAGATGCGACCAGTACGACCATGGTAGAACTTATGAGGCATACCCTTG10.8620689655172413No Hit
ATCCAGAGTGACGACCCTTCATCTTGGCAATCTTCATTTTGCGAGCTCTGG10.8620689655172413No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGCG10.8620689655172413No Hit
GCTTAAAGTTCAATACAAAAAAATGAACCTATAATCTGCATCTGGTAGGTG10.8620689655172413No Hit
TCCTATAGTTTCGTCAATGAGAATAGAGTCTTCAGAAGCTGTCTCTTATAC10.8620689655172413No Hit
GTTTAAACACTGTGGCTACCTTTTCGTATGTGGGAGCGAGTGATTTGCAGT10.8620689655172413No Hit
GACCAAGCCTTATCGGGTGAGTAGGAGCTAACTCAGCAGTGGCAATGTCGG10.8620689655172413No Hit
ACTGTATTGATACCAGTGAACTGCTGAAACGCCTGAAGTCCTGCTCCGGCA10.8620689655172413No Hit
CGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA10.8620689655172413No Hit
TCATAAACCCTAGCAACCACCGTCATCCCCGCCGCCGGAGCAAAAAAACCA10.8620689655172413No Hit
GATATGTCGATTGAATCCGTTATGCTTCCTCCTCCTTGGCGGTTGATGCCA10.8620689655172413No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10.8620689655172413No Hit
GTCTAAGAAGATAGTCGAATACAACAAACTAAACAACGACATAAAGATTTC10.8620689655172413No Hit
GTTCTGGAGAAGGAAGGTGAGACTGTGAACGTAGTAACAAAAGACGATGTG10.8620689655172413No Hit
AATCTGGCCTGATCTGCGGACTGCTCTGATCAGGTGGGGAAGCTTAACTCT10.8620689655172413No Hit
AATCAAAAGAGGAAGATGAAGAGAAATCTTTCGAGTCCATTTGATGGCATA10.8620689655172413No Hit
TCCTTGGAGAAATCAGGAGTAGAATCCATGTGCTTTTTGAGATCCAAGTCA10.8620689655172413No Hit
GTCACGATCACGCTGTTTCTCATACTCACTCTCTCGATCTTGATGTCCTTG10.8620689655172413No Hit
CCTCAATATCCTCATGGTATTATTGATGATATTCCCGCTCTCTCTAAAATA10.8620689655172413No Hit
CCGCAGAGGTTCTGCAGAATCTTAATCTCTCTCTTAATCTTCTTCTTCTTC10.8620689655172413No Hit
GATCAATCTAGAGCATTTGGAGATTCAGTTCTCTAGATTATCTGTCAAGGG10.8620689655172413No Hit
CTGTTATGCTATGATGAAGTGTATCCAATACGGTCACGAGATCGTGGCATT10.8620689655172413No Hit
TGACTGTACCATGGTGTACCTAAGGCAAAAGGAGGATCCTTGGAACTCTAT10.8620689655172413No Hit
GATAGAGCTTGAATTCCAGGGGATCAAGAAATCTTACACCATGCTTCAGAG10.8620689655172413No Hit
TCTCTGATGTATTTGGAAAGACGAATGAAGTTGGTGATGTACAAAGGGAAG10.8620689655172413No Hit
CCACGACAAGACAGCAGAGGATGATGTACCAGTTTCCACTACCTGTCTCTT10.8620689655172413No Hit
GTTCTCTAATACAAGAAGAAATATCCTAAATCATGCACACACACTTTTACA10.8620689655172413No Hit
GTCCTAATCTGAGGATCTTGAAACGTAATCTTATGGATTGGTCTTCTCGGC10.8620689655172413No Hit
GTCTTGTGCAGTAGCAACGACGACGGAATCCACGGCATTTACCTCCGCCGA10.8620689655172413No Hit
AAGCAGAAATGCTAACAACAAAGTGCAAATGACATTCGGGTCAAAAACTCT10.8620689655172413No Hit
CTCAAGAAATACCAGAACTTGGACTTGGCACGAACCTCGTTCGTAGCCCAG10.8620689655172413No Hit
GTAGATATCAAGTGGAATCCCCGCAATTCCATCTTCTGATGGAGAGAAATC10.8620689655172413No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10.8620689655172413TruSeq Adapter, Index 12 (95% over 21bp)
GTTAGAGATTGAAGAGCAGAGTAAACCGGAGAGCCACGAACAGAAACCATC10.8620689655172413No Hit
TCCCTGAGTTTATCAGCGTCGACCACGTTTCTAACGTCAAAAACAAAAGCC10.8620689655172413No Hit
GAGTGAGATCTTCTTAAGAAAACAAATCATGTTTAGTTCTTGATCTGTCTC10.8620689655172413No Hit
ATGCTTGCCTTTCACTGATCTCGAGCAGGCGGCGGAAATTCTTGCTAATCA10.8620689655172413No Hit
ACTACGAGGGGTTCTCTAACGCGACCCTGTGGCCGCTGTACCACGACTGCA10.8620689655172413No Hit
ACAATGGTTACACCATCCAACATCTCTACCTTCCTCACCTTCTTCTCGCCA10.8620689655172413No Hit
TCCCATGACTGATGCGAACATTATGTGAAGCGTTAAGTTCAATAGCTGTCT10.8620689655172413No Hit
GTTACAGTATTATTCTTGCAACAAAAGCCAAAAGTTGACCTTACACACTTC10.8620689655172413No Hit
CATTACACTTGTATCAAAAATTTGAGGAAAAGATCGTACATTATATATGCT10.8620689655172413No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers