FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_2.3410000000a5b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_2.3410000000a5b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520642
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC14990.2879137680018132TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2100.034.28439743
CCGAGCC2300.032.2813714
GCCCACG2250.031.99877218
CGTATGC2300.031.30314441
GTATGCC2300.031.30314442
CGAGCCC2450.030.30495815
CCCACGA2400.029.99884619
CGCCCCC300.005145669429.99884614
TCGTATG2500.029.69885840
ATGCCGT2500.029.69885844
AGACTAC2500.028.79889325
GACTACT2500.028.79889326
AGCCCAC2600.027.69124417
CTCGTAT2650.027.16876639
CGAGACT2800.025.71329723
CTCCGAG2850.024.47274212
TCCGAGC3150.024.28478213
GAGCCCA3150.024.28478216
TGCAATC2900.024.050833
TCTCGTA3050.023.6056538
Produced by FastQC (version 0.11.2)