FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_18.3410000000a6c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_18.3410000000a6c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC31.744186046511628RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21.1627906976744187TruSeq Adapter, Index 12 (95% over 21bp)
CGCCAAGGCTTACCACGAGCAGCTATCAGTCCCTGAGATCACCAATGCCGT10.5813953488372093No Hit
TAGAAAGTCACTGAAACAAGAAAAACAAACCCTATCAAAGGGAAGACAACA10.5813953488372093No Hit
GTCACCTAGTGAATCTGAAAATCATTGCATTGGCACGCTATACATCTGTCT10.5813953488372093No Hit
GATCATCAGGAACAGGTTGAAGTATGCTTTGACTTACCGTGAAGTTATCTC10.5813953488372093No Hit
GGACAGATCAACATCCAATATTAGTCTGAAGTCTGAAATTTACATCGGACT10.5813953488372093No Hit
CAGCTATAGAAGCTAGACATACGATATTACTTCCATGCAGCCATAAGTTCC10.5813953488372093No Hit
CTTTTGCACATTGCCTACTATATCAATCTTCTTCCTGTCTCTTATACACAT10.5813953488372093No Hit
GTTCTTCTCTACACTCTTCACTACAGAACGGTGTGTCTCCTCTGTACATGA10.5813953488372093No Hit
GAGTAGTAGTTACCATCAAACAAACTATAACTGATTTAATGAGCCATTCGC10.5813953488372093No Hit
AGCTTAACATGTTCATTCCAAATCCTTAAGGCATCTGAGAAGAAAGGAAGA10.5813953488372093No Hit
GCTCACCCATGTCCAACTGCTGTTACCATGGAACCTTTCTCCCTGTCTCTT10.5813953488372093No Hit
AAGCATATAACAAACGCAAACAAGTAATCGAAAGTAGATGACAAACAAAGA10.5813953488372093No Hit
GAGCAATGGCTCGCTACTGATACTTCTCCTTATGAGCCTGTCTCTTATACA10.5813953488372093No Hit
TGTAAGGGTTGATTCTTCCGTGAGCACGGTAAGTCCTTCTCCTTTGCTTTG10.5813953488372093No Hit
GAATGAAGACAGAAACAGAGTAGGTAAAATTTAAGCCTGTCTCTTATACAC10.5813953488372093No Hit
ATGTGCACATAACTTACCTCTTCTTATTTTCGCCCGCAGCTATTGTGACTT10.5813953488372093No Hit
ATGCGAAGAAGATTAAAGACGGTAAGAGATTAGAAACAGGTTCAGAGAACT10.5813953488372093No Hit
GTGGAAGTTAGTAACACATCCAATGATCGGGGTGTTCAGAAACAGAGCTAT10.5813953488372093No Hit
GTTTTAAGCATTGACTTGGACAAAACTAACGAAGATGATCTGTTTAAGGCG10.5813953488372093No Hit
GATGAGGATATTATGAGAGTTCTTGAGTTCAATAAGATGGCCTCTCGTATG10.5813953488372093No Hit
ACTTAATCTTGATTCGATTGAACACTTCTGTCTCTTATACACATCTCCGAG10.5813953488372093No Hit
AGCGGTTCCCGCGTTTAAAACTGAGAAAGAGCCCATAACGCAAACACGAAA10.5813953488372093No Hit
ATCCTAGTTAGAAGATGTTAAGAGAGCTGGAAATTGTGTCTGTCTCTTATA10.5813953488372093No Hit
AAGATGGAGTTGATCGATGATGCTTTCCCTCTTCTTAAAGGTGTTGTAGCT10.5813953488372093No Hit
ACGCTCTGCTCTCTCGCGACCCCTACTCTAATCAAAAGACTAAAGGCCCCT10.5813953488372093No Hit
CTGCTTTACTTCCTTTAGAAGAAGAAGATGAGATACAAGCTGTCTCTTATA10.5813953488372093No Hit
ACGTATCCTCTCTTAAGATCCTTGACAGCAACATTCTTGACATTGAATCCA10.5813953488372093No Hit
GTACAGTAGACGCATAACAACACTCTCTCTCAATCTTTTTAAACATGAAAC10.5813953488372093No Hit
ATGACAGACAAGATGTCATTACAAACTTTAGCAAGCTCATCTTCAACTCTC10.5813953488372093No Hit
GAGAAAGTGGTGACATGTTTCCTCAGACCTACACCTGTCTCTTATACACAT10.5813953488372093No Hit
GCCTCCACCAAAGAAGGATGAGAAAATATCAAATGGATCATGTCCACCTCC10.5813953488372093No Hit
TTTTTAACGAACATTGACCAGGGGTTCAAGATTCTGTCTCTGTCTCTTATA10.5813953488372093No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTC10.5813953488372093No Hit
AGCTTATTAGGCTACGCTTCAGGAACAGCTCGTGGTATGGCTCCACGCGCT10.5813953488372093No Hit
CTACAATACAACACCCAAAGACTCAAAAGGGACTCTAAGCTGCTCCTCCAT10.5813953488372093No Hit
ATCTAAAAGAGTCTAATGGAGTGACTTGTTACAGTGTGTATCTGTCTCTTA10.5813953488372093No Hit
TCCAATGGGAACTGAGTGGGATCAATTAGATGCACTTTATGAATTCAATTG10.5813953488372093No Hit
TAATTCTGCCAGTGCTTTTTGAAGTGACCATTGGGGATAACATTGTTGTGC10.5813953488372093No Hit
GTTCAAGAACAAGCGTGTCTTGATGGAGAGTATCCACAAGTCAAAGGCTGA10.5813953488372093No Hit
CAGTAGCACTGTCTTTCAATGACTCTGTTTCATAATCATCAGATTCCTCAT10.5813953488372093No Hit
ATCTCAATCCATAAGACGATGACTCATCTTCATTTCTTTGTATCGCGTATT10.5813953488372093No Hit
CCCGTATTGCTTTTGTTGAGTTTGCGATGGCGGAAAGCGCAATTGCTGCGC10.5813953488372093No Hit
TGCTTGAGCTGCTCTGAGAAATCTCCTAAGGAACGTTTTTGGAGTGGGAGT10.5813953488372093No Hit
GCTCTAGGCGAGGAATTCAAGGGATACGTATTCAAGACTGTCTCTTATACA10.5813953488372093No Hit
GAGTTGAAGAAGAAGAAGAAAGCTCGACTGTGAATGAAGGAGATGACAAAA10.5813953488372093No Hit
GTATAAGCCAAAAATTGTATGTGTTCAGTGAACAAGAACAAAACAACCATT10.5813953488372093No Hit
GTTCAGTGATAATGAGTTACAGAGATAATGAGAACAAGAACTGTCTCTTAT10.5813953488372093No Hit
AACATACAGTTGAAGGTTGGGCTTCAAAGCAAGGAATAGACCTGTCTCTTA10.5813953488372093No Hit
TATCAACGCAGAGTACGGGACGCAGAGTAATCTCTGTCTCTTATACACATC10.5813953488372093No Hit
GGAGAAGAAGGAATGATAGACTTCATGAATGAGAGGAAATTGATGGATTTG10.5813953488372093No Hit
GAATTCGATGGATTGAGCTCCAAACATCCATACCACTTTCCTTCACTAATT10.5813953488372093No Hit
AGAGATAGTGGTGTGACATCAAGCAAGAGGAGATCTTGAACCTTCTCATTA10.5813953488372093No Hit
CCCAATTTGCATTTCTTTCGTCATCATCATCTACTCTTTCTGCGTTTTTCC10.5813953488372093No Hit
ATCAAGGATTGGGGAAAGATAATTTAATGATCAAGACTGCTTTCAAAGATT10.5813953488372093No Hit
ATTGGAAGCTATCCCAAGTTTCTGCAATTTCTATCAGTCTTGTTTCTTCTC10.5813953488372093No Hit
GTTTATGGTTAAGCTTTGTTGAGTGCCCGTACTCTGCGTTGATACTGTCTC10.5813953488372093No Hit
GCTTTTGTGTTGGCTCTACTACTCTGGTTTCATAAGTATTAGATGCTGTCT10.5813953488372093No Hit
CCTTTAGTGTGTCGTGAGTTTGTTGTTGTCTCTGATTTACTACCCTGTCTC10.5813953488372093No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG10.5813953488372093No Hit
TCATAGAGGAACTGTGGACAGTAGTGCCTGTGTATTAAGACTTATTTACTG10.5813953488372093No Hit
GTATACTGTTGTTCATGATGTTAGCGTCGCCAAAATCTGTCTCTTATACAC10.5813953488372093No Hit
CTTTATACCTGTTGTAAAGCTCATTCAATTCAGTGTAGTTTGAGTTTGTCA10.5813953488372093No Hit
ATCTTGTTAATCCTGAGAACATGGAAAGGATGAACCCTAATCCTGTCTCTT10.5813953488372093No Hit
TCTCAAGATGCTTGTAGAGTTGTGAGTCTTTGGATTTGATAATCTTTGAGA10.5813953488372093No Hit
AATGAGAAGTGCTTCTTACCGTAGCAGAGAAGCTGAAGGAAATGGATCTAT10.5813953488372093No Hit
GTATATTTGCATAAACTTAGTTGAAGCTGTCCTTGTACTGTCTCTTATACA10.5813953488372093No Hit
GTACAAAACCAACCATGTTCTTCTCAAACCCATTTTCTTTATCTTTTCTCT10.5813953488372093No Hit
GTCCAAGAAGGTTAGTGTTGGAGAAGTTACTAGTTGCTTTAGACAAATCTT10.5813953488372093No Hit
CTGTGGGTAGATTATTAAACAACCCTTTATGCTGTCTCTTATACACATCTC10.5813953488372093No Hit
GTTTAGTCTTCAGCTTCTTCTACCTCGATTACAGAACCGTGGCTAAAACTA10.5813953488372093No Hit
GTTTCACCATTGCAGGCTTTACGGAAAAGCATCTCAGAGGCAAACGTTTTC10.5813953488372093No Hit
GTCGATGAATCCAGAAAAGCGGTCATTTTCCACCATGATATTCGGCAAGCA10.5813953488372093No Hit
CGTCATGACTGTTGGAGGAGGAGAGGTCTCGGTTCCCTTTTTGCAAGAGAA10.5813953488372093No Hit
ATACGATTCTCTTAAACAAGGGAGCATAAGATTCCGGAAAAAAAAGAGAGG10.5813953488372093No Hit
TCCTAAGGTACTGGACCGACACTTGAAAGCGGCAGGCCGAGGTGGTCTTGA10.5813953488372093No Hit
GCCTACACCTAATGGCTTAAGCCGCTCTTCCCATTTCCTCGCTGACCCATC10.5813953488372093No Hit
CTCAGCAGTTTTGTGTGCGTGTGTGTGAGAGAGATACAGCTGTCTCTTATA10.5813953488372093No Hit
ATCTTAGTCAGAATCATAGCACTAATCTCCTCAGGACTGAAAACCTTAGTC10.5813953488372093No Hit
CACAAAAAGATTCAGAATACTGATTGTGTCCAACTAAAAACTTAAAACCTG10.5813953488372093No Hit
CTCTAGACATTTTGATAGGCATGTGAATGAGAGAAACAGTAGTGAGATTGA10.5813953488372093No Hit
GTAGCATCAAGGCTTTGACCATATTCAACTGGCCTAGATGCAGCAGGAACC10.5813953488372093No Hit
CATCAAGGTTCTAAAATGCTTAAACAAACATTCATTCATCTGTCTCTTATA10.5813953488372093No Hit
GTTCAAGCTCGGAACTTTGCTGCTATAACTGGTGTTAATGCTGGTATTGCT10.5813953488372093No Hit
ATCCAGGGGATACGCAGACTTTGAACTATGATCAAGTGTTTATTCGTGATT10.5813953488372093No Hit
AGTCAGAATTTTCTTAGAAGGACTTCACCGTATATAACCACTGTCTCTTAT10.5813953488372093No Hit
CCACAGGATCCTCTTCGACAAGTACCATCCAGGTTACTTCGGTAAAGTTGG10.5813953488372093No Hit
GGTGATTGTTGAGAAGGCTGAGAAGAGTGATATACCAACTGTCTCTTATAC10.5813953488372093No Hit
GTTCAAATCAAGCTTTTCTTCACTTGGCACTGGCGAGCTGGGAGCGGATGA10.5813953488372093No Hit
ATTCTGTGCTGATTAGTGAACCATCGAACAATAAAACTGAGTCATTTAGTT10.5813953488372093No Hit
TAATAAAATTGCATTATAGATGAAGAAACTGAAGCTACAAATTACATAAGA10.5813953488372093No Hit
TCACAATTCTTCTTAGAAAGTTTCATGTTTGGTTATCTGTTGCTACTTATC10.5813953488372093No Hit
CACGTTAACAACAGAAAATAAATGAAACCCAAAGCGTTGCCTGACAGAATC10.5813953488372093No Hit
TTGTCATCCTCTCTCCCCTGGTTTCGGCCCAACAGGCGCTTCTCCATCTCT10.5813953488372093No Hit
ATCTAGTTTGATCATTTACGAATTGCGTAGATAATCCTCAAGTGATAGAAA10.5813953488372093No Hit
ATCTTTAACAGAGAAATGAATGCCTTTAAGCCGAAAACTTTCCCAGTGAGC10.5813953488372093No Hit
GTAGAGTCAAGACTTAGACAAAAACCAGTCTACAATAGCTGTCTCTTATAC10.5813953488372093No Hit
GATATAGATGCAGCATATACTTGCCTCAAGGAACATTATGGTGTAAAAGAT10.5813953488372093No Hit
ATCTCAGTCACAATGACTCTAGCACCAGCAGTCTTCATGGCAGCAGCACAA10.5813953488372093No Hit
CCTGGAAAGAGAAATATACTAATCACCAGTGCTTTGCCTTACGTCAACAAC10.5813953488372093No Hit
ATCTAGATGTGGTTTGAATCTTATTTTATGGAATGTAATGTGAGGAACCTG10.5813953488372093No Hit
GGGTTAAATTTCCAGATTGTCGTAACTTAATTTTTGTTGTTGTTAGGCTAT10.5813953488372093No Hit
AAAAAGGTCAATATTCCTGTGTGAAACATCGATACAAATTATGCATGTAAG10.5813953488372093No Hit
GTCCATACCCAGCTTATCATGGAATCATCAATAACCACTTTGTCTGTCTCT10.5813953488372093No Hit
CATTGAGGGAGAAAGAGAAGGAGACAGGGGAAACGAGTGAGAGCGATAATC10.5813953488372093No Hit
GTGTTGAATACCAACACGAGTCTTGCATCTACGGCGGCGAGCCACACGATA10.5813953488372093No Hit
GGTTTATACAAAGCAAACCTTTGCAATAAAGCCAAAATACCAACAACTACA10.5813953488372093No Hit
GTTCGCTCACACGCCTAAACATCTCCTGGATTGAGGTTGAGTTACCAATGA10.5813953488372093No Hit
GTTCATAGCAAATTCAAGATCGACTCATTTGCAAAATAAAACTCAAACAAA10.5813953488372093No Hit
CTCGAGAACGGATCGATCATCGACAATCTGGTTTAGAAGAGCACCGACAAA10.5813953488372093No Hit
TTTGTAGACTTTCGCTTAGACGACTCAACTCCTGTCTCTTATACACATCTC10.5813953488372093No Hit
CTATTTTTGTGAATATGAACGGAAACAACTCCCCCACAAGATCCATTATCT10.5813953488372093No Hit
ATGTAGAGAACTTCAATACCTTTCTTCTTGAGCTTCTCAAGGAATGGAGAG10.5813953488372093No Hit
CTGAGGAAGTAAAGGAAGAAGTTGAGAAGAAACCTGTTGCTCGTCGTGGTG10.5813953488372093No Hit
AGCCAAGGCATTCTTGTACGCATTGATGAACATATGCGGTGCAGCAGCAAG10.5813953488372093No Hit
TTCTTAGACAAAGGAACCAATTTACTTTGCGCTTTTGATCATGTTTACGCA10.5813953488372093No Hit
GAGTATCGGCAGCAAGCTTTGATGCAACTGGCCTAGGCGTACGTACAGGCC10.5813953488372093No Hit
CATCATGATCGATCTTGGAACTGGTAACAACAACAACTGTCTCTTATACAC10.5813953488372093No Hit
CTTCTATACAAACAACACAACAAGAGCAGGATATTACAGGGGAGATCAGTT10.5813953488372093No Hit
TGGTATTACCATCGATATTGCTCTATGGAAGTTCGAGACCACCAAGTACTA10.5813953488372093No Hit
TCCTTGGAATGCTTTCCGGCGAGCTTCGGCTTCATCCTTTTGACCCATACT10.5813953488372093No Hit
GTCATAACACAGATGGTCTCTACAGCGCAAAAAAGACAGCTGTTCTGGCAT10.5813953488372093No Hit
GAGTAGAGGGACGAGGATGAAGACGAAAGAGACTACGGAAGCTATGGTTGG10.5813953488372093No Hit
GTGTAAAAATAAGACCAAAACTCTCATCAAAGAGACAAAACGAACATAATG10.5813953488372093No Hit
GGATGTGATTGATCTGGAAGATGACTCCATAGACGCTGAAATCCTCAATTC10.5813953488372093No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT10.5813953488372093No Hit
GCTTCAACCTCAGCTTTATCGTCTCCGGATCATCACCTTGATCAACGATAG10.5813953488372093No Hit
ATCCTGGTCGTGGTGCTCCTCTTGCTCGTGTCACTTTCCGTCATCCTTTCC10.5813953488372093No Hit
CCTCACTGAAGTGCATCGTTAATGGAAAGAATCCACCTTCCTGTCTCTTAT10.5813953488372093No Hit
CCAGATACTTGACTCAGTGATCCAATCCGTCCAAGAGACTCAGTGGCGGCT10.5813953488372093No Hit
ACATTACTTTAGAAGCTTTCTCAACTCTAGCTCTTGCGTTTTCATTATCAT10.5813953488372093No Hit
AATGGGATGAGAGGAGATAATAAACAAAAAAGAAACAAAGGAGCTGTCTCT10.5813953488372093No Hit
ACTTTACCTTTAACAAAAAAAAAATGGCAAGCGCGAATCCAGAAACCGCCA10.5813953488372093No Hit
GAGAAAGGCTATAGCATCCTTTGGATTTGATGCAATGTTTGTGATTTTCTT10.5813953488372093No Hit
TCTTTGATCAGTAACTAACGAAGAAACGAAGATTGCTGTCTCTTATACACA10.5813953488372093No Hit
TTACTACGGTTATCCGAGTAGTAGTTACCATCAAACAAACTATAACTGATT10.5813953488372093No Hit
TACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGC10.5813953488372093No Hit
CGTTAAACAGTGTGCATCACTAAAGGTGAAACACACTAACCTGTCTCTTAT10.5813953488372093No Hit
TCTTTGGTCAGTGGCCAGATCCGAGTGCCCAACCACTTTGAATCACTTTCA10.5813953488372093No Hit
CTTTAATCGTGTGTGTTTTAAAACCTACTTCTCTATCTTTTCGTAGCCATG10.5813953488372093No Hit
GACCTGGAGATATCAGAAGTGAGAATGCTGACATGAGTAACGATAAATCCC10.5813953488372093No Hit
CAAAGATGCATACTAAAAAAGTAAAAAGACAAACCGCTCAAGATTCATAGG10.5813953488372093No Hit
TTTTAGGAGCGAGTAGAAGAAAGAAGATCTTGAACAGCATCCATGCTTGGC10.5813953488372093No Hit
CTCGTGCTCGGGTTCGTTGGTCGCCATAGTATTTACTGAGATTTCCTCGTG10.5813953488372093No Hit
GTTGATCCCTATGGAAACCTTGTGCTTGGTTGGTTTGAGACAATTTGAGAT10.5813953488372093No Hit
TTCCCAACCTTCAGTCCTTCCTTGGACACATTCTGGAACTTTTTTGTCTTC10.5813953488372093No Hit
CTTATCAACAAGGTGAGAACCAATGAATCCAGCTCCTCCTGAGATCAAAAT10.5813953488372093No Hit
CATTAAGACCATGTTCTTTTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5813953488372093No Hit
ATTAAAAGATCCATGGCTTATTCGTGAGAACTTGTAAATTTAGTAACTGAG10.5813953488372093No Hit
TCTCAAAGGTAATCAAGACACCTCCTTTGGTACCACATGGTATATCCCTCA10.5813953488372093No Hit
GTCCAGAGTTGCTTGTAGGAATATGCTTCTTCTTTTTTACCCTAAAATATG10.5813953488372093No Hit
TACTTACACATGCATGGCTTAATCTTTGAGACAAGCATATGACTACTGGCT10.5813953488372093No Hit
GTCTACAAGATTGGTGGTATTGGAACGGTGCCAGTGGGACGTGTTGAGACT10.5813953488372093No Hit
ATCTCAAATTTCACCATCTGTTGACCCGACCCGTAACCCTGTCTCTTATAC10.5813953488372093No Hit
CTCATATCTGCATCAACAACTGTTTTTCAATATTACAGAGCAAAAGTGAAA10.5813953488372093No Hit
TCCCAAAGGAGTTCTATCACGAGGAGCATCGACCCACTCATTTCCTGGAGT10.5813953488372093No Hit
TCCTTTTCCTTTGGCATTGTGGATGTTGACGATATCACGAGAGTTAACACC10.5813953488372093No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGA10.5813953488372093No Hit
CTCCACATCCGATCCTCGCTTCCCACAATCACATCTTGTCAGCTCCGGAGA10.5813953488372093No Hit
TGACCACACAGAATATTTGATACTCTTGTGACTGTCTCTTATACACATCTC10.5813953488372093No Hit
AAGCACTCTCCACCAGCTTTCTCAATTCTAGCCCTGTCTCTTATACACATC10.5813953488372093No Hit
TTATAACCATGCTTAAGTTCAAGACCAACAAACGAACCTCTCCGCCGTTTG10.5813953488372093No Hit
ACACCGATAATTCAATTTCATCTAATCCCAAAAAGAGATCTGATCAGAGGA10.5813953488372093No Hit
CTATAGTACTCACTCACATAGCGCGAGTAATTACAGCAGTCTCTTATACAC10.5813953488372093No Hit
CTCTCAAGGACTATGAGATCCATGACGGCATGGGTCTTGAGCTTTACTACA10.5813953488372093No Hit
AATTTTTAGCCGTCGTCAATACAAAAGACAATTTGAAACACCTCTTACAAT10.5813953488372093No Hit
CAAATAATGAGCAAGCTTAATGTTGTAGCCTGTAGGTTTGTGTCTATACCT10.5813953488372093No Hit
GTACACCTTTAGCACCATTTTTCTTTCTTCCTGTCTCTTATACACATCTCC10.5813953488372093No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)