FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_14.3410000000a682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_14.3410000000a682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807570
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC19840.24567529749743056TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA2900.035.68813319
CGTATGC3050.034.67065441
GCCCACG3050.033.9329818
TATGCCG3200.032.3423743
ATGCCGT3400.031.10161644
GTATGCC3400.030.43987842
CTCGTAT3500.030.21299739
CGAGACT3400.029.77814323
TCGTATG3600.029.37374540
CTCCGAG3700.028.57986312
AGCCCAC3800.027.8277617
CGCGCGC1300.027.69112610
CCGAGCC3750.027.59882414
ACGAGAC3950.026.7710122
GTCGCGC1400.025.7131888
GAGACTA3850.025.71318824
GGGTCCG901.1905286E-824.99893229
CCTGCGC450.001226543924.9989328
CGAGCCC4150.024.93869615
TCGCGCG1450.024.8265279
Produced by FastQC (version 0.11.2)