FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_13.3410000000a668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_13.3410000000a668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310885
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC4750.15278961673930874RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG659.094947E-1238.0748818
GTCGCGC504.861613E-835.998078
GGTCGCG504.861613E-835.998077
CGCGCGC504.861613E-835.9980710
CCCACGA702.0008883E-1135.35524419
CGAGACG653.6561687E-1034.61352523
CGTATGC754.7293724E-1132.9982341
TATGCCG754.7293724E-1132.9982343
TCGCGCG551.1237353E-732.7255179
CGCGCTT651.3973477E-831.15217412
TCGTATG852.0190782E-1029.11608740
CTATATA406.0714583E-428.1915111
CCGAGCC903.929017E-1027.49852614
TCTCGTA1004.5474735E-1126.99855438
GCGCGCT755.6143108E-826.9985511
TGCTATC856.4464984E-926.4691733
GACGAAT856.4464984E-926.4691726
CTCGTAT957.366907E-1026.05123539
CGAGCCC1058.367351E-1125.71290815
GGGGCTA709.275318E-725.71290636
Produced by FastQC (version 0.11.2)