FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_12.3410000000a65c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_12.3410000000a65c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTGTGTTTTCGCACCAAGGAGCCTCTTGTACTCCAGAGTCAAGTTCTC11.0869565217391304No Hit
GGTCAGTATTTACTGGCTTCCCTAAGATCCCTTCTTTAACAAGACCCCATA11.0869565217391304No Hit
GTTCTAATCAAAACACAAGAGTAACCAGAATACTTAGATCTAAGCTCGTTC11.0869565217391304No Hit
TCATTAGTGTTCTTTACGTTGAATATCGAACTCGTAAAGAACGAGAAAAAT11.0869565217391304No Hit
GCTCTAAAGTGTCCATCTGTTGAAGTAGCTGTTGTTGATATCTCTGTGCCA11.0869565217391304No Hit
GCTCTAGGTTTCCGGCCACCAAAATCCGCAAGTCTTCACTCGCCGCCGTGA11.0869565217391304No Hit
GTGTAGGAGTCGGAGGTGGTGGTGAAACTGGTGGAGTGGGACTTGGCACAG11.0869565217391304No Hit
GATCGCATGATGCTTACCTTCTCGGTGTTCTCTTCACCAAAGGTTTCTGAT11.0869565217391304No Hit
ATCTGATCCCTCTACCAGGGTAAATTTTCTGGCCACTGAATCGGCAAAGCT11.0869565217391304No Hit
TATCAATCCAATTACATTGCCAAATACAATTTCGTTTTGCCTAAACTCTCC11.0869565217391304No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATATCGTATGCCGTC11.0869565217391304No Hit
GGAAGAAGAAGCATCAAGAGGATGAATTAGGTAAGTCAGCACTAACGCCAC11.0869565217391304No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGT11.0869565217391304No Hit
CAAAGTTTGAGATCGAGACTATCCCGGGGCCAACCATTGGAAGCTGTCTCT11.0869565217391304No Hit
GGTATCATGCTTGGTGCTGACCAACCTGTTATCCTCCACATGTTGGATATT11.0869565217391304No Hit
ATATTACCAGAAACAAGAGTCAATACACAAACGAACTAGCTAATAGAAATC11.0869565217391304No Hit
TTGTGCATGTTTCCAGGTGATGCGGAGATGAAAATGTCGCTGTGCATACTA11.0869565217391304No Hit
TACCTCGCCAGCTGCGGTCTCGGAATCAAGTGGCACCCCACTCATTCAGTT11.0869565217391304No Hit
TTCTTTCTTCTTCTCCCTAATCGTTTCTTTTAGCAGCTCTTTCTCTCTCAC11.0869565217391304No Hit
GAGAAGATCCACCACAAGGTCTCTGAGAATTGGATCAGCGCCACTCTTCTC11.0869565217391304No Hit
GGTATCAACGCAGAGTACGGGGAGGTATCCTGTCTCTTATACACATCTCCG11.0869565217391304No Hit
GTTTTGGCAGGTTCTTCAGCTTTCACTTCCTCCACCGGTATTTCTTTAGTC11.0869565217391304No Hit
CATATAGTTCATGTGTTCTACATGTTCCTCTCTTGGTGGAACACAATCTTT11.0869565217391304No Hit
CTTCTATCGACCTCAAGACTCGAAGGACAATTCAAGAACTGTCTCTTATAC11.0869565217391304No Hit
ATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA11.0869565217391304No Hit
GTTTATGTTTTTTTGGTCTCATATCGTTTTTTTGTGTGATTACATGAACAT11.0869565217391304No Hit
CACCTGGATTTGAGTCTAACTCTCTCTGTGGTTATTACGCTTGAGATTCTT11.0869565217391304No Hit
CTGCTCCAGCTCCTGCTCCTATCGTTAGTGCTCCTTCGGATCTGTCTCTTA11.0869565217391304No Hit
GTCCCATCTCCGGCTTCTTCACCTTTCCTCGAAGCTGCTCTTAGTTTACGC11.0869565217391304No Hit
CCTTTACCTCAACTTCAGTAGTTGGCTTCTTCTTCTCCTTCTTCTTCTTCC11.0869565217391304No Hit
GAAGAGTACAGAGATAAATTACAAAAAAGCAGAGAATAAGCTGTCTCTTAT11.0869565217391304No Hit
GTACGAGATGGCTTCACACCTCGCGGTGCTTCCACCTCTCGCCTATCGAAG11.0869565217391304No Hit
TCAACGCAGAGTACGGGATGAGCAATGCCTGTCTCTTATACACATCTCCGA11.0869565217391304No Hit
TTCTTATCCTTACTCTGAAGAGCATCAAGAGTCGCCACTTCCTTGAGATCC11.0869565217391304No Hit
GGGTGACTGGCAGAAGAGGCAGGTGCAAACACATTGAAGAAGATGATGACA11.0869565217391304No Hit
GACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAG11.0869565217391304No Hit
GCACTGCAGCTGGACGCCCACCATTACATTCACCACCGTTGATCCTCCTCG11.0869565217391304No Hit
GAAACCCGCCGTGGAAGCCTCCGTGACAGCTCCAGTGGAGAAGGCTGATGA11.0869565217391304No Hit
GCCCAAAAGCAGTGGCTCGTTGCTCGTCCCTTGGTAATGCAGCTACTGTGA11.0869565217391304No Hit
TGTAAAAGGAGTCTGGGGATAGGTGGTTTTCATGGCTGGTATAATGCTGTC11.0869565217391304No Hit
CCCTTGTATCTGTACTGGCCTTGGTTTGCTTTACAACGTTAGCCGATTCTT11.0869565217391304No Hit
CTTGTAGCTTATCCACGAATCCAGACCAATCACCCATTCCTAAGAGACGGC11.0869565217391304No Hit
AACCAAAGCTCAAGTCACTCTTTGCCCAATGCTCTCAAGCAGGGATATAGA11.0869565217391304No Hit
GTACGGTGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATAT11.0869565217391304No Hit
AACCAGATTTCTCAAGAATAAATGAAGTTGTGATCTCTCTGTACAAATCTA11.0869565217391304No Hit
CTCCAATGCAGTACTCACATCCATGTCCTCTGGGATCACTGCTGCCTCAGC11.0869565217391304No Hit
ATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCGG11.0869565217391304No Hit
TGTTTATCTATTTGCTCTTTTCAATGAAGACCTTAAACCAGGACCAACCTC11.0869565217391304No Hit
CTTCCTTCTCTGTGTCACGTCCCACTCTCACTGACGCTCCACGGTTCGCGA11.0869565217391304No Hit
GGTTTTGGTGGGTTAATTCTGGTAGACAAGAATCAAACAAACAATGGGAGA11.0869565217391304No Hit
GTTATACTCAACATTGATCTGCTCATTGACGGCGGCTTCGCACTCAGGGGA11.0869565217391304No Hit
CTTGCACCACATAGATTTCTCGTTTGAACTCAGTGGCTAGACACTCGATTG11.0869565217391304No Hit
ACATTGAGTTTAAGACATAATCAGAAGAAACAATAAACAACCACACAAGTC11.0869565217391304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0869565217391304No Hit
CCATTGAGTAAAGGCTATCAACTTTACTCCCAACCAGCAGCTGGTGCCCCT11.0869565217391304No Hit
GACAGAGGGCTTGGCAGCAGCAGAAGAGAGTCTCGACCTCTTCTTTGCCAA11.0869565217391304No Hit
CTCTATGAACCGGGCTCTTGTATATCCCATTGCTCATTATCTCCATCTGAT11.0869565217391304No Hit
CCGTACGACGAGGTCGGGATCCCGCACCAGACGCTGTCTCTTATACACATC11.0869565217391304No Hit
GTCCCGGGTTCAAGGTATGTCCCTTCGAATGGTAGATGTATGCATCACCTT11.0869565217391304No Hit
AAGTTGTAGAGGCGAAGAAAGAGGAAGAAGTAGAAGAAAAGAAGCTGTCTC11.0869565217391304No Hit
CATATGTCTCGTTTCCAGAGACTGAAGGCCAGCAGTTTATTGTTAGTGGCA11.0869565217391304No Hit
ATACAATGTCAGCCAAACCGGAATTAGAGACAACACAGCTAACGACCACCA11.0869565217391304No Hit
CTCTCCGAAGGGGTGTTTTCTCCCCCAAACTCTCTGTCTTTCTTTTTTTTC11.0869565217391304No Hit
GCTGAATACTCCTCTGTGTTGTAACGGTATATCTCGTTGATTTTCTTAATA11.0869565217391304No Hit
ACCGCGTTCCTTCTTTGCCTGCGCTTCGGATTCTCAACGGAACGTCTTTGT11.0869565217391304No Hit
TACCACGATATTGTAGGCGAGCCCTCACGGTGAACGATCTGTGTCTTTTGG11.0869565217391304No Hit
CTCCAGTGGTTGTGGAGGAAGAGAAGAAGCCAGAGGCAGAGGGAGAGAAAC11.0869565217391304No Hit
TCTAAAGGGTCTTGGAGGTTTCTCTAACACAGCAGAGAAGAAAACTTCTTT11.0869565217391304No Hit
CTTCTATATAGTCCTCTCGGAGGTTTTCCGATGAAGATCGTCACTGATCTC11.0869565217391304No Hit
TGCCTTTGGTTTTTGGTTCCAATATTTGTGGAACACAACAAAAGTACTTAG11.0869565217391304No Hit
GCTAAGACCATTCCTAAAGGACTTCACTGCCTTCCTCTGCGCCTCACTACA11.0869565217391304No Hit
GCCTTCTGTTATTGTGATTGGTGGTGGTATATCAGGTCTTGCTGCTGCTAG11.0869565217391304No Hit
CCCTAAATCTCTGCTCCGAGTCTGAGCCACACCGCCCAGAACCGAGCAAAA11.0869565217391304No Hit
AGATAACTCTAAAAGATAAAACCTTTCAAGAAGATTTCTTCTTCCTTCTTA11.0869565217391304No Hit
ATCCCGGATTATAACCGCTCCTTCTGGTCGGAGAATTCTATCCCTGTCTCT11.0869565217391304No Hit
CACATACAGTCAATGTTTCGATGTCTATTGAAAATCTGTCTCTTATACACA11.0869565217391304No Hit
CACATAAAGACTGAGAGTAAGAGAGTAAGAGAGAAAGTTATTAGTCTATCT11.0869565217391304No Hit
ACCCAGGGCGGACGAGCCTTCCCCTGGAAACCTTAGTCTTACGGTGGACAG11.0869565217391304No Hit
CTCTAACAGTTAAGTGGAATGGAAAAGAGTACACCGTCCGTATCTGTGCCG11.0869565217391304No Hit
GGCTTTGGCGTCTTTCATGTCAGAAGCATTCATCATTTCCCTAACAAAAAG11.0869565217391304No Hit
ACAGAAGATTGTGAAGAAGGTTTTGAAGCTTCAGAAGGCGAAAGAGAAGAC11.0869565217391304No Hit
ACACCACCCGATACACTCTCCAACACCGAGTTCCATCCACAATGCGTCACA11.0869565217391304No Hit
CTTTAACCATTTTGCTTAATCATCAGACTCTTTTTCTTTCTTCACTTCCTC11.0869565217391304No Hit
GGTGTGTACAATGGAAAGACTTTCAACCAAGTGGAGATCAAGCCTGAGATG11.0869565217391304No Hit
TTCTCACGCTAAAGGAATTGTTCTTGAGAAAATTGGTATTGAGGCTAAGCA11.0869565217391304No Hit
ACTTAACACAAATACATAAAAAGGTCCATTTTAGTGGTTGGTGAAAATGCG11.0869565217391304No Hit
GTGTAAATGATCTTAGGACCTATCTTTTTTTCTTCTATCACCGAACTTTGA11.0869565217391304No Hit
CCGTGTGACGGCTCAACAAAGCAGCCTACACGTCCTACCTATTTAAAGTTT11.0869565217391304No Hit
GGATCGCATTGAGGGAGAAAGGTGTTGAATTTGAGTACAGAGAAGAAGATC11.0869565217391304No Hit
GTTTCTCTCTATACACCAAAACATTCCTCATTTCATTTGTAGGATTTTGTT11.0869565217391304No Hit
CCCTTGAACCATGTCAAAGAGAAAGACTAAAGAGCCAAAGGTCGATGTTGT11.0869565217391304No Hit
GTGTTAAGTAATATCTCCATTGTCCTTGTTTTGCTTTCTTCTGAACAAAGA11.0869565217391304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCT50.045.023
GTGTTTT50.045.06
GCCTCTT50.045.024
TCAAGTT50.045.042
TTCGCAC50.045.011
TTGTACT50.045.029
CCTGTGT50.045.03
GTACTCC50.045.031
GTCAAGT50.045.041
TGTTTTC50.045.07
CTCTTGT50.045.026
TTTTCGC50.045.09
GAGCCTC50.045.022
CTCCAGA50.045.034
CCAGAGT50.045.036
ACCAAGG50.045.016
GTTTTCG50.045.08
ACTCCAG50.045.033
AACCTGT50.045.01
TCCAGAG50.045.035