FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_3_1.3410000000a590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_3_1.3410000000a590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC74.117647058823529No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC42.3529411764705883TruSeq Adapter, Index 12 (95% over 21bp)
TAACAGAAGCAATTCCAGCATTCACACCAGTAATTGCAGCAAAATTCCGAG10.5882352941176471No Hit
GTGTAGAGGAGTAATGGATACAAGCTAGAGTACAGTGTGTTAGCAGAAACG10.5882352941176471No Hit
TTATAGTACTTATGGATTTGGTAAAGGGGACATTGGACCTGTCTCTTATAC10.5882352941176471No Hit
TACGAACAGTGCTCTTAAGTATTCAAGCTCTTCTGAGTGCACCAAACCCAG10.5882352941176471No Hit
CTCCAACACAGCCTCCGTCACATCCACCAACACAGCCTCCAACACCGCCAC10.5882352941176471No Hit
GTCTTACACAAATGTGAACAATCAAAACTGATAAAGAAAACAAAGTCTTTT10.5882352941176471No Hit
TCTTAAAGGACCAAAGAACTCGCGTGAAGCAGTGAAGCATTTCGGACCTGC10.5882352941176471No Hit
CTCAATTCCCCAGCACACACGTCAAGGAAAAATGGGGAAAGAATCTCTACC10.5882352941176471No Hit
ATCCTAAATACCACAAAACATAAACAAAAGCTCTCTTTCACTATCCTGATG10.5882352941176471No Hit
CAAAGGAGCTGATGTTATTGTCTTTAATACCGGACATTGGTGGACTCATGA10.5882352941176471No Hit
CATTGGATCTCCCATGATCTCTAGCCCTTCCAATGTCTGTGTCAAGCTTCT10.5882352941176471No Hit
GTGCTGGGCCTGCTCGCCAAGGCCACCGCCCTTGCCGTCGCCGCTCAGAGC10.5882352941176471No Hit
CAAAGGTTCCATCTTTTGAGGAGTTGGAGCAAACTCCGGACGCTAAGTATT10.5882352941176471No Hit
GGTTGGATCAATTACAAGCACCACGGGAATAGATTCGAGCTTGTAGTATGT10.5882352941176471No Hit
GCTCACGAAAGCCGAGACACAAGCTAAATACGGATGGTGATTTGCCGCTAT10.5882352941176471No Hit
CTCTCTGTCTCTCTCTTTTGCATTATTTAGGAACTCAAAACACACAACAGA10.5882352941176471No Hit
GGTCAAAAACTCTTGAGAATTTCAGCAATGTCTAAAAACTGTCTCTTATAC10.5882352941176471No Hit
TCAGAAGGCGAAAGAGAAGACTGCACCAAAGAGTTAGAAGAAAACTTTTTG10.5882352941176471No Hit
GCCTTTAAGAGGTCCTTCTGAGGCATGCTTAATGGCTGCCTTAACATCTTC10.5882352941176471No Hit
GTGTACACTCCGTGGTCCAACTTGAAGAAAACTGCCTCCATGGATGTTGGT10.5882352941176471No Hit
TATCAACGCAGAGTACGGGGATTCTTTCTTTCTTAACCATGTATCATATAC10.5882352941176471No Hit
GCCTCACTGGTCTCTGGTTCCTCTGCTGCCTCGATTTCCTCGTCGGCTACT10.5882352941176471No Hit
AGATAATCCTGACCTTGGTTATGATGCTGCTAAAGGAGAATATGTGGACAT10.5882352941176471No Hit
ATCATGCTAACATCAGCTCCAGCAGCCAGTGCTTCCATGAATTCCTTGGTT10.5882352941176471No Hit
TTACAAGAGAAGGAGAGGTCTTCTCTGATTTATTTGCATTTTTTTCAAGTC10.5882352941176471No Hit
ATCTTATAGAAGGGGAAGATGAAGCAGAAGAAGAATTACGGCGAGACAAGT10.5882352941176471No Hit
CTAATATTGTGGTTCCAGTTGTTTTGTTTAATGAATAATAACCTGTATCTA10.5882352941176471No Hit
TCACTGTCCACACTCCCAAGACTTGGCACACCGTCACCGGAAAAGGCTTGT10.5882352941176471No Hit
ATGTTGTTTAAAGAAGCTTGTCCCAACTATTACAGCTGACTCTTATACACA10.5882352941176471No Hit
CCACAGTATAGTGCAGCGAGATGGCGACGATTGGATGCACTAACGTCTTTG10.5882352941176471No Hit
GTACATCACAGCGGCTGCATAAGCCTTTACAATCTTATCACTTACTTTCAA10.5882352941176471No Hit
CCCAAGAGTTTTGAGCCCATTCTCACAAGACGAGATTATAGAGAAACCCAC10.5882352941176471No Hit
CTAGCAGGCAGTGCCCAAGAAAAACCTTCTCAAGGTGAAGTCATTGCAGTA10.5882352941176471No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGACGTC10.5882352941176471No Hit
CGGGGCGCCTTGGTGATTCATGATAACTATACGAATCGCACGGCCTTGCGC10.5882352941176471No Hit
TGATTATGATAAGCAATCTTAATAACAAAATCTGAATTGCACTTTCATACC10.5882352941176471No Hit
CCATATCTCCGTTTATTTCGTTGAACATATAATTGCTGTCTCTTATACACA10.5882352941176471No Hit
AGGAAGAAGTAGAAGAAAAGAAGACAGAGGAAGCTCCAGTGGTTGTGGAGG10.5882352941176471No Hit
GAAGAAGCCAGAGGCAGAGGAGGAGAAACCCGCCGTGGAAGCCTCCGTGAC10.5882352941176471No Hit
ATCAGTAGCATCCATGGAAGCTCTTAGCACTCTTTTCGAGAGTTTCACACA10.5882352941176471No Hit
GAATAACCGTGTTTCTCGGGATCAACTTTGTCATCACTCCTCCTACAGTCT10.5882352941176471No Hit
TCATTTCCCTGAGTAATGACTTGAGAAGCAAAACCTCTTATAACTGTCTCT10.5882352941176471No Hit
GCTGATACTCTGCAACAAGATCATTCATGTTACTCTCTGCTTCAGTGAACT10.5882352941176471No Hit
TTCTTCTTCTTGGAAGTCTGGTTGTCGATTGTGATCACAATCTTTCCCGGT10.5882352941176471No Hit
GTACTGAGGGTCTCGTTCGTGGAAGGAAAGTCCTCAACACTGGCGCTCCAA10.5882352941176471No Hit
GCAGAAACAAGTGATTGTTGAGAAAATCGGTGAACCGGGTCAAACCCATGA10.5882352941176471No Hit
GAGTAGCAGGCAGAAGGAGCACGACAGCTATTGGAGCCATGTAAAGGAGGA10.5882352941176471No Hit
GTATCTACCGGAAGGGTTCGTGAGTCGTACTAGTGATAGAGGTTTCGTGGT10.5882352941176471No Hit
CTTTTATATCGCATTGAATCAAAGACAGAGAATTTCTCTGAGAGCATGACC10.5882352941176471No Hit
GTAGGCGGCCTGCCAAGTCTGGTTTTAAAGCCTGCGGCTCAACCGCAGAGA10.5882352941176471No Hit
TTTATATACTGCGCCTATTCCAAATGGTACTTGTACTTCTCTATTTTCTCT10.5882352941176471No Hit
TTGTTTTGTTGTGTATTGTTCTGGGTATCGCTGCATACTTATACCTGTCTC10.5882352941176471No Hit
GTCTCACACTGTGCCAATCTACGAGGGTTTCTCTCTTCCTCATGCCATCCT10.5882352941176471No Hit
ACACAAACACACAGATAACTTTTACAGCATCCTCACTTCACCAAGTTGATA10.5882352941176471No Hit
GTTTCGGAAAGAGAAGAAACAAGAGTCACACACTCTGTGTAAGATGTGGCC10.5882352941176471No Hit
CATATTTCTCTGCTTGTTTGATGCAATTCTTAAATTCCTCTGTTATTCCAT10.5882352941176471No Hit
GGATGGAGGTACTTTCAAGTTGACTCTTCACTTCACCGAGGATTATCCGAA10.5882352941176471No Hit
ACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACC10.5882352941176471No Hit
CTGTTTTTGTGGTTAGCTCCTTCTTGCCAACCAACCATGAGCTCCCAGATC10.5882352941176471No Hit
GCCTTGACGTTGTCAATAGTGTCAGAGCTTTCAACCTCCAAGGTAATGGTT10.5882352941176471No Hit
CTTCGCTGAAGGTCGGAATCTCTTCCATCAGCTTCAACCTAACTATCTCTT10.5882352941176471No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTCATATAACACCCCGCACC10.5882352941176471No Hit
GCTATTAGCAGAAGACCTATTAGGGACTACTCCATAGTTGTTACCATACCC10.5882352941176471No Hit
GTTTTTGTTAAAGTTTGTCTCCTTTGTTGTGTCTTTTAATATGGTTTGTAA10.5882352941176471No Hit
GAACTGTAATCGGAAGAGACACGGAAGTTAGCGGACGAGAGAGCCCTGTCT10.5882352941176471No Hit
ATACTGTTGATGATGCTCTTCAGCTCTGTAGAATTTCTTAGCTGGCAAGAT10.5882352941176471No Hit
CTTCAGGAAGCGGCTGAAGCATACCTCGTTGGATTGTTTGAAGACACCAAT10.5882352941176471No Hit
CTATTATCCGCCGTGTCCTCGGCCGGATTTAGCTTTAGGTGTACCGGCTCA10.5882352941176471No Hit
TTATACTCATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTGT10.5882352941176471No Hit
GCGTTGGAACAGCTGAAGATCGGTCCAAGTTTCCGCACGACGGTGGCTGTC10.5882352941176471No Hit
GTGTAATCAAAAGAAGAACTTGGATTTTTAGACTTGGCTTCCTGAAAGTAA10.5882352941176471No Hit
CTGAGTATCTGAAGAATGTATGGAAAGTGATCAACTGGAAATATGCAAGCG10.5882352941176471No Hit
CTAATTTCTTAATGTAACCATCTGTTTCTCTATGTAGCCTGTCTCTTATAC10.5882352941176471No Hit
ATTCTCCACCATCCACAAGCTTGAGAGGCTCCCGTCGCAGAAGATTGTTAC10.5882352941176471No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGC10.5882352941176471No Hit
GACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGG10.5882352941176471No Hit
CTCCTCTTCTCCTCCACCGCACAGATCCAAGACATTTTGATCTGTCTCTTA10.5882352941176471No Hit
GAAAAAAACTGTCTTTTGAAAGACAAAAGAAGAAGAACTGTCTCTTATACA10.5882352941176471No Hit
TCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATG10.5882352941176471No Hit
ATCTTCATCTCCGGTTCAATTTTCCGGTTTGATGCAATGATGAGATTTTAT10.5882352941176471No Hit
CAAGAGGGACAAATGTTGACCACATGATTTGAGGAAGAACGGAGAGTCCAC10.5882352941176471No Hit
GCCTCGGCTTTTGGCTCAGCTTCAGCCTCCACTGGCCAGTAAGAAGTCTTC10.5882352941176471No Hit
CTTATACACATCTCCGAGCCCGCGAGACTACTTGCAATCTCGTATGCCGTC10.5882352941176471TruSeq Adapter, Index 12 (95% over 21bp)
CTCCAAGACATTGAAGCGAGATGTGAAAGCTTTGAATGAAATCAAAGCCTC10.5882352941176471No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5882352941176471No Hit
CCAGAGTTCCCGTGAGTCAATTCGCGCCGCCGAATTATCAGCAAGCTAATG10.5882352941176471No Hit
CAGCCGCAGCAGCCATGTCTCCCGTTGTTCCCAACCTGTTAAGCAGAGTCT10.5882352941176471No Hit
AATCGATATTATTACATTCCAATTCCTTACCAATATCCCTCAAGGAAAATC10.5882352941176471No Hit
CTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTACCTGTCTCTTATA10.5882352941176471No Hit
AGATAAGAAAGGACTAGTGGAGAAGATCAAGGAGAAGCTCCCAGGACCTGT10.5882352941176471No Hit
CAATAATACATCCACACAATAGCATATGAAACAGAAATCAAACTATTGATA10.5882352941176471No Hit
TATCAACGCAGAGTACGGGGTAGCGGCTGAAGCACTGCACGCCCCAGGTCA10.5882352941176471No Hit
CAAGCAAACTTTGTCCGGGAAAGGAGAGTTTGGGTCTGATCAGGCACTTCT10.5882352941176471No Hit
GTACAAGAACAACGTTCCCGAGCACGAGACACCAACGGTCGCAACAGAGGA10.5882352941176471No Hit
TCTTATACACATCTCCGAGCCCACGAGGCTAGATCCTATCTCGTATGCCGT10.5882352941176471No Hit
CCTTAAAACCGCAATTAAAATCGAAAAAAAGGAGGAAGAAGCAATGCTTAG10.5882352941176471No Hit
CTCATACGCCGAACGTTCAATTAACTCAAAATCATCAATCGTTCCAACTAA10.5882352941176471No Hit
ACCTATGAGTGTTGGGAATTTGGAAGAGTTGATTGATGAGAATCCTGTCTC10.5882352941176471No Hit
CACTCATAAAAAAAAAAAAAAAAAAAAAACATACTCTTTCTTTGATACCCC10.5882352941176471No Hit
GTTCCAATAGCCCACTCGAAATGATGATATCCAGCATTCCTGTCTCTTATA10.5882352941176471No Hit
ACTTGGCTCAAATAAAAGGAAACGTTCTCGGGACACACTTATCCTCTCCGC10.5882352941176471No Hit
CTGCACTTATGAGGAAAACACTCAATGGGGAAAGGAGATGGGTGTGAAATA10.5882352941176471No Hit
GTCGATACCTTCAGGGTTCCTGATTGCACCAGTAATGTCGCTTACTGCAAC10.5882352941176471No Hit
CACACACACCTACTGTCGTGGATGCTATGAGTCGGCTGCTTCAACGAGCTG10.5882352941176471No Hit
TCATTACAGCTCCCAAGTTTGCTGGAGATGCAACAAATTCTAAGCAGAACG10.5882352941176471No Hit
CTTAGAGACTTTGCCAAGGACAGCATTCGCCTCGTTAAGCGTTGTCATAAG10.5882352941176471No Hit
ATATCATCTCTTTCTACGCTGCTTTCGTAATTTGGAAATATAACCATATCC10.5882352941176471No Hit
ATGTTAGCCACAGCAGCCTTCGCCGTACGAAGGCCTGTCTCTTATACACTC10.5882352941176471No Hit
GAATTACTCTCTCCTCCAAGAATGTCAAGAACTTGGAAAAAGTGTGTACTG10.5882352941176471No Hit
ACCTATCTCACTCTAAAATCTCTCTCTGCCAATCTCATCTTCAACCTCTCT10.5882352941176471No Hit
CAAAACAATATAAAGAAAGGAATGAACGTATCCACTGAAAATATCACACCA10.5882352941176471No Hit
TAAGTACACCATTCACTTAAGCAGTGTTGAGATCTTCATTCCAAGGAAGAT10.5882352941176471No Hit
GCGGTGTTGGTTGGACTTGGTGGCGTAAGAGTGACGTGATCGGTAAACAAG10.5882352941176471No Hit
GCTGCGGATTATGGGTTTTGATGCATTCACTTTCCGTGAGGATTGAGGATG10.5882352941176471No Hit
ATTCCATAGGACGTGTCTCAAAATTTCTATCTTATGCAATACCTTTCTAGT10.5882352941176471No Hit
AAAGTATACGTCTCAAAGAGACGATTTAACATAAAAAGAAAGCAGAACATG10.5882352941176471No Hit
GTAGACAGGAGAGTGAAACCTCTATCCGAGGAAGTTGATGATCACCAAATG10.5882352941176471No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCTCCTATCTCGTATGCCGTC10.5882352941176471No Hit
AATAAATAATCGACTCTGTAATTCAAAGCAATAAGCAGAAGCTGAAATTGA10.5882352941176471No Hit
ACCAAGTACTATACGACGACGAAGATGTGGAGTCCAAGTTGCTCAGAGCAA10.5882352941176471No Hit
ATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACAT10.5882352941176471No Hit
ATCTTCAACCGATTTCTTCCCCATAGCTTTCTCTATTGCTTTCCATGGCGA10.5882352941176471No Hit
GAACCATGACAATGTGCTCGAGCATAGACCATTAACGGACTATGGAATCGA10.5882352941176471No Hit
GTGCTGTAGCCATCGGCTAGAGAGTTGGCTCCACCTCCGTAACGGGTGTAG10.5882352941176471No Hit
CATAAGCTTTGCATCCTCATAGCATTGCATTTGGACTTTGTACATCAGTTC10.5882352941176471No Hit
GTATTTTAACTTCACTGAAGATTCTAATTGGCTTGTGGAGCTGTCTCTTAT10.5882352941176471No Hit
CTCTACCTGATTCCACTCTCACCTCTTCTAATTTCCCGATCACAACCTCAA10.5882352941176471No Hit
GCATTGAATCTGTAGATTCTTTGGGCAATATGGTCATTTTAACAATATGGA10.5882352941176471No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTGTCTCTTATACACATC10.5882352941176471No Hit
CTATTAGCACATTTATCTAATTCGATTAACCATTCTGGATTAATTGTGAAA10.5882352941176471No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.5882352941176471No Hit
AGGTTTAACAAAAGTTAATGGTGGTTTTGTTGTAACAGGTGGATCAGCTAA10.5882352941176471No Hit
TTTTTAAGCTATACACTCGTACGCCAGAGGGCCAAAACAGAACACAATAAG10.5882352941176471No Hit
CCCTTGAAGAGTGACTTGGCTCCTTCATTCTTGAGGATCTGCTTGAAGGCT10.5882352941176471No Hit
ACTCTTGTATGCTGTTTCACTTCCTAGCTCCACAAGTGTCTCTTATACACA10.5882352941176471No Hit
AAGTATTTCCGAGTTAAGATGGATATTAAGCTCTATGAATGTTACTAAGCT10.5882352941176471No Hit
CAGAGAAACCAATCGAAGAAACAGAGGAAGCTCAAGTTGAAACTCCGGAGG10.5882352941176471No Hit
ATCTTGAGCTCTCTGTATTCTTGACAAAGAGAGCAACAAGGGCATAGGACA10.5882352941176471No Hit
GAATTGGATTGTCTGTAGGAGGTATTGTATTAGCTGGAGGATGGGTATTCA10.5882352941176471No Hit
CTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTT10.5882352941176471No Hit
CCTGAGGACTGTACGAAATTGCACCCAGAGGAGAAGATTTTAGTTTGATAT10.5882352941176471No Hit
ATCAGAGGGCATGGGAAGTGGTTAGGGAAACAAGAGCAGCTAGCCTGTGGG10.5882352941176471No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC10.5882352941176471No Hit
GTGGATACACTGTGGCATCCGGCGCGGGGTTCTGGAACGTGGCTATGGATA10.5882352941176471No Hit
GACATGATGATAATAACAATAAGTCTCCACTTCTTTCACTCAGCTCAAGAC10.5882352941176471No Hit
GTTTAAGTCTGGTCTTGTTTTGAGTTCTAGTTGATTTCATGTTGGTTATGT10.5882352941176471No Hit
GTGATAACTTGATCAACGGTGTTCTTCCAAAGAAGAAACCACAGATATATG10.5882352941176471No Hit
CCTCCGGGTACGTACGGAAACTACGACAAGTGCCAGTGCTACGCTAGCCTC10.5882352941176471No Hit
GGAAGAAGCAGAAGATGATGACAATGAAGGAGATGACTCAGACAATGAATC10.5882352941176471No Hit
ATATTAAGCTCTACGAATGTTACCATTAGAGTTACTAATACGTCTGTCTCT10.5882352941176471No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGATTTTGTTAA10.5882352941176471No Hit
CTTAGAGAGTGATGTTGATGAACAACTTCTCATCTATATTCCTTTTAACCA10.5882352941176471No Hit
GCATATGATCCAAAGAGATCAGATCCCATACCAGCAATGTCACCAACATTG10.5882352941176471No Hit
CCTTTACTAGCATTCTCGATCAATTTTTATCAATTAACCCCAAAGATTAGA10.5882352941176471No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTATTTTTTTT10.5882352941176471No Hit
CACTAGAACTGAGTATGACAGAGGAGTGAACACTTTTTCTCCAGAAGGAAG10.5882352941176471No Hit
TCACTCCATCAATCCTCTCCGACCGTATGAGGATTAATGGGTCTCTTGCGA10.5882352941176471No Hit
CTTCAAACCAAAACTAATCAAAGAGATGGAAAACAATACAGCATACTCACT10.5882352941176471No Hit
GGCGTATGGCGGATGCCTAGGCTTTCGGAGGCGAAGAAGGACGTGGTAAGC10.5882352941176471No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers