##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_2_95.3410000000a576.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.8421052631579 0.0 0.0 0.0 0.0 0.0 2 31.68421052631579 0.0 0.0 0.0 0.0 0.0 3 34.8421052631579 0.0 0.0 0.0 0.0 0.0 4 33.21052631578947 0.0 0.0 0.0 0.0 0.0 5 33.73684210526316 0.0 0.0 0.0 0.0 0.0 6 33.68421052631579 0.0 0.0 0.0 0.0 0.0 7 36.31578947368421 0.0 0.0 0.0 0.0 0.0 8 32.8421052631579 0.0 0.0 0.0 0.0 0.0 9 36.578947368421055 0.0 0.0 0.0 0.0 0.0 10 32.68421052631579 0.0 0.0 0.0 0.0 0.0 11 36.21052631578947 0.0 0.0 0.0 0.0 0.0 12 35.36842105263158 0.0 0.0 0.0 0.0 0.0 13 33.94736842105263 0.0 0.0 0.0 0.0 0.0 14 32.578947368421055 0.0 0.0 0.0 0.0 0.0 15 32.68421052631579 0.0 0.0 0.0 0.0 0.0 16 33.21052631578947 0.0 0.0 0.0 0.0 0.0 17 32.31578947368421 0.0 0.0 0.0 0.0 0.0 18 32.78947368421053 0.0 0.0 0.0 0.0 0.0 19 31.94736842105263 0.0 0.0 0.0 0.0 0.0 20 32.05263157894737 0.0 0.0 0.0 0.0 0.0 21 36.94736842105263 0.0 0.0 0.0 0.0 0.0 22 34.68421052631579 0.0 0.0 0.0 0.0 0.0 23 34.73684210526316 0.0 0.0 0.0 0.0 0.0 24 36.526315789473685 0.0 0.0 0.0 0.0 0.0 25 35.526315789473685 0.0 0.0 0.0 0.0 0.0 26 33.473684210526315 0.0 0.0 0.0 0.0 0.0 27 34.05263157894737 0.0 0.0 0.0 0.0 0.0 28 32.10526315789474 0.0 0.0 0.0 0.0 0.0 29 33.1578947368421 0.0 0.0 0.0 0.0 0.0 30 32.26315789473684 0.0 0.0 0.0 0.0 0.0 31 33.89473684210526 0.0 0.0 0.0 0.0 0.0 32 34.31578947368421 0.0 0.0 0.0 0.0 0.0 33 33.78947368421053 0.0 0.0 0.0 0.0 0.0 34 32.36842105263158 0.0 0.0 0.0 0.0 0.0 35 34.578947368421055 0.0 0.0 0.0 0.0 0.0 36 35.73684210526316 0.0 0.0 0.0 0.0 0.0 37 33.526315789473685 0.0 0.0 0.0 0.0 0.0 38 33.473684210526315 0.0 0.0 0.0 0.0 0.0 39 33.05263157894737 0.0 0.0 0.0 0.0 0.0 40 35.26315789473684 0.0 0.0 0.0 0.0 0.0 41 34.10526315789474 0.0 0.0 0.0 0.0 0.0 42 32.473684210526315 0.0 0.0 0.0 0.0 0.0 43 31.842105263157894 0.0 0.0 0.0 0.0 0.0 44 34.526315789473685 0.0 0.0 0.0 0.0 0.0 45 33.94736842105263 0.0 0.0 0.0 0.0 0.0 46 33.36842105263158 0.0 0.0 0.0 0.0 0.0 47 33.78947368421053 0.0 0.0 0.0 0.0 0.0 48 34.68421052631579 0.0 0.0 0.0 0.0 0.0 49 32.526315789473685 0.0 0.0 0.0 0.0 0.0 50 32.36842105263158 0.0 0.0 0.0 0.0 0.0 51 35.05263157894737 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2113 1 0.0 2113 2 0.0 2113 3 0.0 2113 4 0.0 2113 5 0.0 2113 6 0.0 2113 7 0.0 2113 8 0.0 2113 9 0.0 2113 10 0.0 2113 11 0.0 2113 12 0.0 2113 13 0.0 2113 14 0.0 2113 15 0.0 2113 16 0.0 2113 17 0.0 2113 18 0.0 2113 19 0.0 2113 20 0.0 2113 21 0.0 2113 22 0.0 2113 23 0.0 2113 24 0.0 2113 25 0.0 2113 26 0.0 2113 27 0.0 2113 28 0.0 2113 29 0.0 2113 30 0.0 2113 31 0.0 2113 32 0.0 2113 33 0.0 2113 34 0.0 2113 35 0.0 2113 36 0.0 2113 37 0.0 2113 38 0.0 2113 39 0.0 2113 40 0.0 2113 41 0.0 2113 42 0.0 2113 43 0.0 2113 44 0.0 2113 45 0.0 2113 46 0.0 2113 47 0.0 2113 48 0.0 2113 49 0.0 2113 50 0.0 2113 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 0.0 24 0.0 25 1.0 26 2.0 27 0.0 28 0.0 29 0.0 30 1.0 31 0.0 32 1.0 33 2.0 34 1.0 35 2.0 36 1.0 37 3.0 38 1.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.63157894736842 21.052631578947366 15.789473684210526 10.526315789473683 2 0.0 26.31578947368421 52.63157894736842 21.052631578947366 3 15.789473684210526 15.789473684210526 42.10526315789473 26.31578947368421 4 0.0 31.57894736842105 52.63157894736842 15.789473684210526 5 5.263157894736842 47.368421052631575 36.84210526315789 10.526315789473683 6 26.31578947368421 36.84210526315789 26.31578947368421 10.526315789473683 7 21.052631578947366 42.10526315789473 21.052631578947366 15.789473684210526 8 5.263157894736842 42.10526315789473 36.84210526315789 15.789473684210526 9 26.31578947368421 15.789473684210526 5.263157894736842 52.63157894736842 10 15.789473684210526 21.052631578947366 47.368421052631575 15.789473684210526 11 21.052631578947366 42.10526315789473 31.57894736842105 5.263157894736842 12 21.052631578947366 36.84210526315789 31.57894736842105 10.526315789473683 13 15.789473684210526 21.052631578947366 36.84210526315789 26.31578947368421 14 10.526315789473683 21.052631578947366 42.10526315789473 26.31578947368421 15 31.57894736842105 36.84210526315789 5.263157894736842 26.31578947368421 16 26.31578947368421 21.052631578947366 31.57894736842105 21.052631578947366 17 5.263157894736842 31.57894736842105 31.57894736842105 31.57894736842105 18 10.526315789473683 52.63157894736842 21.052631578947366 15.789473684210526 19 15.789473684210526 15.789473684210526 26.31578947368421 42.10526315789473 20 10.526315789473683 36.84210526315789 31.57894736842105 21.052631578947366 21 21.052631578947366 26.31578947368421 15.789473684210526 36.84210526315789 22 21.052631578947366 42.10526315789473 10.526315789473683 26.31578947368421 23 42.10526315789473 31.57894736842105 10.526315789473683 15.789473684210526 24 15.789473684210526 31.57894736842105 21.052631578947366 31.57894736842105 25 26.31578947368421 31.57894736842105 21.052631578947366 21.052631578947366 26 15.789473684210526 42.10526315789473 31.57894736842105 10.526315789473683 27 15.789473684210526 47.368421052631575 26.31578947368421 10.526315789473683 28 26.31578947368421 26.31578947368421 15.789473684210526 31.57894736842105 29 0.0 47.368421052631575 36.84210526315789 15.789473684210526 30 31.57894736842105 36.84210526315789 5.263157894736842 26.31578947368421 31 31.57894736842105 31.57894736842105 15.789473684210526 21.052631578947366 32 0.0 47.368421052631575 26.31578947368421 26.31578947368421 33 10.526315789473683 63.1578947368421 26.31578947368421 0.0 34 21.052631578947366 31.57894736842105 26.31578947368421 21.052631578947366 35 21.052631578947366 21.052631578947366 42.10526315789473 15.789473684210526 36 10.526315789473683 52.63157894736842 15.789473684210526 21.052631578947366 37 21.052631578947366 26.31578947368421 47.368421052631575 5.263157894736842 38 15.789473684210526 21.052631578947366 36.84210526315789 26.31578947368421 39 0.0 31.57894736842105 15.789473684210526 52.63157894736842 40 21.052631578947366 52.63157894736842 15.789473684210526 10.526315789473683 41 26.31578947368421 47.368421052631575 26.31578947368421 0.0 42 26.31578947368421 31.57894736842105 15.789473684210526 26.31578947368421 43 15.789473684210526 31.57894736842105 26.31578947368421 26.31578947368421 44 26.31578947368421 21.052631578947366 31.57894736842105 21.052631578947366 45 21.052631578947366 26.31578947368421 21.052631578947366 31.57894736842105 46 26.31578947368421 10.526315789473683 52.63157894736842 10.526315789473683 47 26.31578947368421 31.57894736842105 21.052631578947366 21.052631578947366 48 15.789473684210526 21.052631578947366 47.368421052631575 15.789473684210526 49 21.052631578947366 5.263157894736842 36.84210526315789 36.84210526315789 50 10.526315789473683 15.789473684210526 57.89473684210527 15.789473684210526 51 10.526315789473683 21.052631578947366 47.368421052631575 21.052631578947366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.5 31 1.0 32 2.0 33 3.0 34 2.5 35 2.0 36 3.0 37 4.0 38 3.0 39 2.0 40 2.0 41 2.0 42 2.0 43 2.0 44 2.0 45 2.0 46 1.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.5 59 1.0 60 0.5 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 19.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGTTATAGCAGTTCCAACAGACTAAGACACATGTACTAATACTCAGCCTG 1 5.263157894736842 No Hit GTTCACTCCAAACACAAACACAGCAGTAATCAAAAATCTGTCTCTTATACA 1 5.263157894736842 No Hit GTGTAGGTACACAAGACCTAGCAAAACCTGTTTACAGACAACAGCAAGGTA 1 5.263157894736842 No Hit ATCAATATCAGTATGTGCTCAAATTATCAGACATTATTCAAGCTGTCTCTT 1 5.263157894736842 No Hit CACTTTATCTCGGGAGATTTCAGATTTGTCGATTTGTTCAGCAATGGCGCC 1 5.263157894736842 No Hit TAATTACTCTGAACCCTACTGAACCAAGACTAAACCGGCTAAAACCGATAA 1 5.263157894736842 No Hit GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGCTGTCTCTT 1 5.263157894736842 No Hit ACTATGTCTTTGTTGGTAAACCGGACAACAAAACGATACTTAGGAGTATTG 1 5.263157894736842 No Hit TCTTTGCAGTGATGAGTTTCTTGTCTGTTCATATTATTCGTTGTCTTTTGT 1 5.263157894736842 No Hit GATCCTAACTTTACAGCAGCTTTAGCAGCAGCTGTTACCGGAAAATTGTAT 1 5.263157894736842 No Hit GTCATGATCAAGCTGCAGAACACAATACAAACTGTCTCTTATACACATCTC 1 5.263157894736842 No Hit TCTTAAGTCCTCTCACCTCTAATCCTCCTCGCAAGTTGAATATCCTTAGGC 1 5.263157894736842 No Hit GTCTATATGTGATACACACAACACAAATTGCTGAAATTAAGATGAGAATCC 1 5.263157894736842 No Hit AACCAGAAGGATGCATATGTTGGTGATGAAGCACAAGCCAAGAGAGGTTTT 1 5.263157894736842 No Hit GTTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT 1 5.263157894736842 No Hit CCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACCTGTCTCTT 1 5.263157894736842 No Hit GTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTACGCAGCCGTTGCGAGGTA 1 5.263157894736842 No Hit ATGACACACTAATTGTTACAACAAGGACAGTAAGTAAAACAGCTGTATCTT 1 5.263157894736842 No Hit GAAAACGAAGAAGTCTCATGAAGGTATCAACAGTAGGTTGACTCTTGTTAT 1 5.263157894736842 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 5.2631578947368425 33 0.0 0.0 5.2631578947368425 34 0.0 0.0 5.2631578947368425 35 0.0 0.0 5.2631578947368425 36 0.0 0.0 5.2631578947368425 37 0.0 0.0 5.2631578947368425 38 0.0 0.0 10.526315789473685 39 0.0 0.0 10.526315789473685 >>END_MODULE >>Kmer Content pass >>END_MODULE