FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_92.3410000000a540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_92.3410000000a540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGATTCTTGCTTAAGAGGGTCTTGCATTTGCATTTGCATCTGCATTTG15.88235294117647No Hit
ATGAAGTACCTTTGAAAGATAGAAACTTTAGTCCCAAAAGAAGCAAACAAC15.88235294117647No Hit
TCATAATCTTGACAAACTCTTCATAGTTAATCTGACCATCTCCATCAACAT15.88235294117647No Hit
GCTAATACGAATCATCACCGTCGACTAATCTAATCCTGTCTCTTATACACA15.88235294117647No Hit
CTTTAAAAGGACTAGCCAGTGAAGCGAATGAAGTCTCCTAAGCAATCATAA15.88235294117647No Hit
AGTGAACTCTGTCTACTACAGGGATGAGATAGGAAACCTGTCTCTTATACA15.88235294117647No Hit
CCAACATCTCGACCAACCCGCCTTTGATACTGTAATGAGAATGAAGCTCTA15.88235294117647No Hit
CCGTTACCTGATCGTTGTGAATTTTTGAGGTTTAGTGATTCTGGATTGTGA15.88235294117647No Hit
AATAAGAACTTCAAAAGAAAATAATCCTGTTACCCCCAAAGTATCTATCCA15.88235294117647No Hit
CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTA15.88235294117647No Hit
CTCCATATGAAGGCAAATCAGTGTTGGAACTTGGAGCTGGTATTGGTCGTT15.88235294117647No Hit
CTCCCAAAGCAAGAAGTATCTTCTTCCTAGGTCCCATTGGTATGCTGTCTC15.88235294117647No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC15.88235294117647No Hit
ACATAGTGGCACAGATTGTATCTGCAAGCATTGCTGGTGACATTGTTAAAG15.88235294117647No Hit
TATCAGCAAAGATGAACCAGCTAGCCTCTCTTCTTTCAAGAGATTGTCAAG15.88235294117647No Hit
ACCTTCCTCTTTATGATCTCTCATGATCATCGTTCTTCTCCACCTGTCTCT15.88235294117647No Hit
GTGCCATATATGTTTACACTAGAATGGTCAACAAGTTCAAGATTGAAGGAT15.88235294117647No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers