##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_2_88.3410000000a50c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.04347826086956 0.0 0.0 0.0 0.0 0.0 2 33.26086956521739 0.0 0.0 0.0 0.0 0.0 3 35.95652173913044 0.0 0.0 0.0 0.0 0.0 4 32.43478260869565 0.0 0.0 0.0 0.0 0.0 5 35.869565217391305 0.0 0.0 0.0 0.0 0.0 6 35.52173913043478 0.0 0.0 0.0 0.0 0.0 7 35.43478260869565 0.0 0.0 0.0 0.0 0.0 8 38.130434782608695 0.0 0.0 0.0 0.0 0.0 9 36.608695652173914 0.0 0.0 0.0 0.0 0.0 10 37.21739130434783 0.0 0.0 0.0 0.0 0.0 11 35.52173913043478 0.0 0.0 0.0 0.0 0.0 12 36.869565217391305 0.0 0.0 0.0 0.0 0.0 13 36.17391304347826 0.0 0.0 0.0 0.0 0.0 14 38.08695652173913 0.0 0.0 0.0 0.0 0.0 15 36.0 0.0 0.0 0.0 0.0 0.0 16 37.0 0.0 0.0 0.0 0.0 0.0 17 36.69565217391305 0.0 0.0 0.0 0.0 0.0 18 36.91304347826087 0.0 0.0 0.0 0.0 0.0 19 35.08695652173913 0.0 0.0 0.0 0.0 0.0 20 35.95652173913044 0.0 0.0 0.0 0.0 0.0 21 35.95652173913044 0.0 0.0 0.0 0.0 0.0 22 35.43478260869565 0.0 0.0 0.0 0.0 0.0 23 37.52173913043478 0.0 0.0 0.0 0.0 0.0 24 36.17391304347826 0.0 0.0 0.0 0.0 0.0 25 35.08695652173913 0.0 0.0 0.0 0.0 0.0 26 35.95652173913044 0.0 0.0 0.0 0.0 0.0 27 35.869565217391305 0.0 0.0 0.0 0.0 0.0 28 33.0 0.0 0.0 0.0 0.0 0.0 29 35.08695652173913 0.0 0.0 0.0 0.0 0.0 30 32.91304347826087 0.0 0.0 0.0 0.0 0.0 31 34.91304347826087 0.0 0.0 0.0 0.0 0.0 32 34.391304347826086 0.0 0.0 0.0 0.0 0.0 33 35.95652173913044 0.0 0.0 0.0 0.0 0.0 34 35.52173913043478 0.0 0.0 0.0 0.0 0.0 35 36.91304347826087 0.0 0.0 0.0 0.0 0.0 36 34.95652173913044 0.0 0.0 0.0 0.0 0.0 37 36.34782608695652 0.0 0.0 0.0 0.0 0.0 38 35.95652173913044 0.0 0.0 0.0 0.0 0.0 39 35.73913043478261 0.0 0.0 0.0 0.0 0.0 40 36.34782608695652 0.0 0.0 0.0 0.0 0.0 41 35.17391304347826 0.0 0.0 0.0 0.0 0.0 42 33.21739130434783 0.0 0.0 0.0 0.0 0.0 43 35.26086956521739 0.0 0.0 0.0 0.0 0.0 44 35.08695652173913 0.0 0.0 0.0 0.0 0.0 45 34.95652173913044 0.0 0.0 0.0 0.0 0.0 46 35.391304347826086 0.0 0.0 0.0 0.0 0.0 47 35.04347826086956 0.0 0.0 0.0 0.0 0.0 48 32.65217391304348 0.0 0.0 0.0 0.0 0.0 49 33.65217391304348 0.0 0.0 0.0 0.0 0.0 50 34.08695652173913 0.0 0.0 0.0 0.0 0.0 51 34.08695652173913 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2105 1 -3.0 2105 2 -5.5 2105 3 0.0 2105 4 -3.0 2105 5 0.0 2105 6 -1.0 2105 7 -3.0 2105 8 -1.0 2105 9 0.0 2105 10 0.0 2105 11 0.0 2105 12 0.0 2105 13 0.0 2105 14 0.0 2105 15 -12.5 2105 16 0.0 2105 17 0.0 2105 18 0.0 2105 19 0.0 2105 20 0.0 2105 21 0.0 2105 22 0.0 2105 23 -3.0 2105 24 -3.0 2105 25 -1.0 2105 26 0.0 2105 27 0.0 2105 28 -13.5 2105 29 -5.5 2105 30 0.0 2105 31 0.0 2105 32 -3.0 2105 33 0.0 2105 34 -4.0 2105 35 -1.0 2105 36 -4.0 2105 37 -3.0 2105 38 -4.0 2105 39 0.0 2105 40 0.0 2105 41 0.0 2105 42 0.0 2105 43 0.0 2105 44 0.0 2105 45 0.0 2105 46 0.0 2105 47 0.0 2105 48 -3.0 2105 49 0.0 2105 50 0.0 2105 51 0.0 2213 1 3.0 2213 2 5.5 2213 3 0.0 2213 4 3.0 2213 5 0.0 2213 6 1.0 2213 7 3.0 2213 8 1.0 2213 9 0.0 2213 10 0.0 2213 11 0.0 2213 12 0.0 2213 13 0.0 2213 14 0.0 2213 15 12.5 2213 16 0.0 2213 17 0.0 2213 18 0.0 2213 19 0.0 2213 20 0.0 2213 21 0.0 2213 22 0.0 2213 23 3.0 2213 24 3.0 2213 25 1.0 2213 26 0.0 2213 27 0.0 2213 28 13.5 2213 29 5.5 2213 30 0.0 2213 31 0.0 2213 32 3.0 2213 33 0.0 2213 34 4.0 2213 35 1.0 2213 36 4.0 2213 37 3.0 2213 38 4.0 2213 39 0.0 2213 40 0.0 2213 41 0.0 2213 42 0.0 2213 43 0.0 2213 44 0.0 2213 45 0.0 2213 46 0.0 2213 47 0.0 2213 48 3.0 2213 49 0.0 2213 50 0.0 2213 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 2.0 27 1.0 28 0.0 29 0.0 30 2.0 31 0.0 32 1.0 33 1.0 34 1.0 35 2.0 36 1.0 37 2.0 38 5.0 39 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.08695652173913 21.73913043478261 17.391304347826086 34.78260869565217 2 21.73913043478261 30.434782608695656 21.73913043478261 26.08695652173913 3 8.695652173913043 30.434782608695656 30.434782608695656 30.434782608695656 4 8.695652173913043 21.73913043478261 60.86956521739131 8.695652173913043 5 4.3478260869565215 47.82608695652174 30.434782608695656 17.391304347826086 6 8.695652173913043 60.86956521739131 13.043478260869565 17.391304347826086 7 26.08695652173913 30.434782608695656 30.434782608695656 13.043478260869565 8 8.695652173913043 39.130434782608695 21.73913043478261 30.434782608695656 9 17.391304347826086 21.73913043478261 13.043478260869565 47.82608695652174 10 17.391304347826086 34.78260869565217 17.391304347826086 30.434782608695656 11 30.434782608695656 43.47826086956522 17.391304347826086 8.695652173913043 12 4.3478260869565215 30.434782608695656 34.78260869565217 30.434782608695656 13 39.130434782608695 17.391304347826086 26.08695652173913 17.391304347826086 14 21.73913043478261 21.73913043478261 47.82608695652174 8.695652173913043 15 13.043478260869565 26.08695652173913 34.78260869565217 26.08695652173913 16 21.73913043478261 8.695652173913043 30.434782608695656 39.130434782608695 17 21.73913043478261 34.78260869565217 21.73913043478261 21.73913043478261 18 30.434782608695656 26.08695652173913 13.043478260869565 30.434782608695656 19 52.17391304347826 8.695652173913043 26.08695652173913 13.043478260869565 20 17.391304347826086 30.434782608695656 26.08695652173913 26.08695652173913 21 13.043478260869565 17.391304347826086 34.78260869565217 34.78260869565217 22 8.695652173913043 47.82608695652174 34.78260869565217 8.695652173913043 23 17.391304347826086 30.434782608695656 21.73913043478261 30.434782608695656 24 13.043478260869565 21.73913043478261 30.434782608695656 34.78260869565217 25 21.73913043478261 21.73913043478261 39.130434782608695 17.391304347826086 26 26.08695652173913 34.78260869565217 30.434782608695656 8.695652173913043 27 21.73913043478261 21.73913043478261 39.130434782608695 17.391304347826086 28 4.3478260869565215 34.78260869565217 26.08695652173913 34.78260869565217 29 34.78260869565217 17.391304347826086 30.434782608695656 17.391304347826086 30 30.434782608695656 21.73913043478261 26.08695652173913 21.73913043478261 31 21.73913043478261 21.73913043478261 34.78260869565217 21.73913043478261 32 21.73913043478261 30.434782608695656 30.434782608695656 17.391304347826086 33 21.73913043478261 21.73913043478261 21.73913043478261 34.78260869565217 34 8.695652173913043 34.78260869565217 26.08695652173913 30.434782608695656 35 30.434782608695656 30.434782608695656 34.78260869565217 4.3478260869565215 36 13.043478260869565 26.08695652173913 21.73913043478261 39.130434782608695 37 4.3478260869565215 43.47826086956522 26.08695652173913 26.08695652173913 38 13.043478260869565 26.08695652173913 34.78260869565217 26.08695652173913 39 13.043478260869565 26.08695652173913 30.434782608695656 30.434782608695656 40 4.3478260869565215 21.73913043478261 39.130434782608695 34.78260869565217 41 26.08695652173913 17.391304347826086 26.08695652173913 30.434782608695656 42 8.695652173913043 13.043478260869565 47.82608695652174 30.434782608695656 43 21.73913043478261 13.043478260869565 26.08695652173913 39.130434782608695 44 13.043478260869565 13.043478260869565 60.86956521739131 13.043478260869565 45 21.73913043478261 21.73913043478261 34.78260869565217 21.73913043478261 46 4.3478260869565215 13.043478260869565 47.82608695652174 34.78260869565217 47 17.391304347826086 8.695652173913043 34.78260869565217 39.130434782608695 48 4.3478260869565215 21.73913043478261 43.47826086956522 30.434782608695656 49 13.043478260869565 17.391304347826086 30.434782608695656 39.130434782608695 50 8.695652173913043 26.08695652173913 47.82608695652174 17.391304347826086 51 8.695652173913043 17.391304347826086 39.130434782608695 34.78260869565217 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.0 25 0.5 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 1.0 35 2.0 36 1.5 37 1.0 38 2.0 39 3.0 40 2.0 41 1.0 42 3.0 43 5.0 44 4.0 45 3.0 46 3.0 47 3.0 48 2.0 49 1.0 50 1.0 51 1.0 52 1.5 53 2.0 54 1.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 23.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACTGAAACATCTAAGTAGCTAAAGGAAGGGAAATCAACCGAGACCCCGTT 1 4.3478260869565215 No Hit TCATTTGAACGTTAACGTGTTTACTCGTGAAACATTGTTAGACTGTCTCTT 1 4.3478260869565215 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAACAACTGTCTCTTATA 1 4.3478260869565215 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 1 4.3478260869565215 No Hit CCCCATTCAGAAATCTTCGGATCAATTCATATTTGCTAATCCCTGTCTCTT 1 4.3478260869565215 No Hit CTTTCATTTTCTTGCTGGTAGTGCAACTTGGGCATTTGTAATGTTTGATAT 1 4.3478260869565215 No Hit CCCGTAGGACGTATGCGGTATTAGCGTTCCTTTCGAAACGTTGTCCCTGTC 1 4.3478260869565215 No Hit ACCAAAGACAACAATCCCGACATTCTCATTCTCAAGATCTGTCTCTTATAC 1 4.3478260869565215 No Hit GGCTAATTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCCTGTCTCTT 1 4.3478260869565215 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT 1 4.3478260869565215 No Hit GGGTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCACACACCTATGTATT 1 4.3478260869565215 No Hit AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA 1 4.3478260869565215 No Hit CTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACCTGTCTCTTATAC 1 4.3478260869565215 No Hit CTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACAAGCTGTCTCTTA 1 4.3478260869565215 No Hit CAAATAATCAACGTTCCATATGGATTACTGGAGTAACACCAATGGCTGTCT 1 4.3478260869565215 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCCG 1 4.3478260869565215 No Hit CCATCATCCAATCCACAATAAATCGAATGAAACCTGTCTCTTATACACATC 1 4.3478260869565215 No Hit TATCAACGCAGAGTACGGGACACACTTATGACTGTCTCTTATACACATCTC 1 4.3478260869565215 No Hit AGTTTCAACCTTCTTGACTATGACAAGAGTCGCGACCTGTCTCTTATACAC 1 4.3478260869565215 No Hit TGGTAGGAGAGCGTTCTAACATCATAGAAGCTGAGCCTGTCTCTTATACAC 1 4.3478260869565215 No Hit GAATCATCTCGATGCAACGCAATTTCTCCCTCATCATGCCCTGTCTCTTAT 1 4.3478260869565215 No Hit GAAAAGAAGGAATGTTAGGGTTCTTTAAGGGCTTGCAGGCTCAAATCTTGA 1 4.3478260869565215 No Hit AATTACTATCGCGACGCGGGCATCAGTAGGGAAAAACTAACCTGTCTCACG 1 4.3478260869565215 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 4.3478260869565215 25 0.0 0.0 4.3478260869565215 26 0.0 0.0 4.3478260869565215 27 0.0 0.0 4.3478260869565215 28 0.0 0.0 4.3478260869565215 29 0.0 0.0 4.3478260869565215 30 0.0 0.0 4.3478260869565215 31 0.0 0.0 4.3478260869565215 32 0.0 0.0 8.695652173913043 33 0.0 0.0 8.695652173913043 34 0.0 0.0 13.043478260869565 35 0.0 0.0 13.043478260869565 36 0.0 0.0 13.043478260869565 37 0.0 0.0 21.73913043478261 38 0.0 0.0 21.73913043478261 39 0.0 0.0 30.434782608695652 >>END_MODULE >>Kmer Content pass >>END_MODULE