FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_88.3410000000a50c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_88.3410000000a50c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTGAAACATCTAAGTAGCTAAAGGAAGGGAAATCAACCGAGACCCCGTT14.3478260869565215No Hit
TCATTTGAACGTTAACGTGTTTACTCGTGAAACATTGTTAGACTGTCTCTT14.3478260869565215No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAACAACTGTCTCTTATA14.3478260869565215No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC14.3478260869565215No Hit
CCCCATTCAGAAATCTTCGGATCAATTCATATTTGCTAATCCCTGTCTCTT14.3478260869565215No Hit
CTTTCATTTTCTTGCTGGTAGTGCAACTTGGGCATTTGTAATGTTTGATAT14.3478260869565215No Hit
CCCGTAGGACGTATGCGGTATTAGCGTTCCTTTCGAAACGTTGTCCCTGTC14.3478260869565215No Hit
ACCAAAGACAACAATCCCGACATTCTCATTCTCAAGATCTGTCTCTTATAC14.3478260869565215No Hit
GGCTAATTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCCTGTCTCTT14.3478260869565215No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT14.3478260869565215No Hit
GGGTACTTAGATGTTTCAGTTCCCCTGGTTCGCCTCACACACCTATGTATT14.3478260869565215No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA14.3478260869565215No Hit
CTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACCTGTCTCTTATAC14.3478260869565215No Hit
CTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACAAGCTGTCTCTTA14.3478260869565215No Hit
CAAATAATCAACGTTCCATATGGATTACTGGAGTAACACCAATGGCTGTCT14.3478260869565215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCCG14.3478260869565215No Hit
CCATCATCCAATCCACAATAAATCGAATGAAACCTGTCTCTTATACACATC14.3478260869565215No Hit
TATCAACGCAGAGTACGGGACACACTTATGACTGTCTCTTATACACATCTC14.3478260869565215No Hit
AGTTTCAACCTTCTTGACTATGACAAGAGTCGCGACCTGTCTCTTATACAC14.3478260869565215No Hit
TGGTAGGAGAGCGTTCTAACATCATAGAAGCTGAGCCTGTCTCTTATACAC14.3478260869565215No Hit
GAATCATCTCGATGCAACGCAATTTCTCCCTCATCATGCCCTGTCTCTTAT14.3478260869565215No Hit
GAAAAGAAGGAATGTTAGGGTTCTTTAAGGGCTTGCAGGCTCAAATCTTGA14.3478260869565215No Hit
AATTACTATCGCGACGCGGGCATCAGTAGGGAAAAACTAACCTGTCTCACG14.3478260869565215No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)