FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_87.3410000000a4fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_87.3410000000a4fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATCTGTCTCTTATA22.3529411764705883No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTC11.1764705882352942No Hit
CAGGTTGATATTCCTGTACTAGAGTATGTAGTGATGCTGTCTCTTATACAC11.1764705882352942No Hit
ATTTTCTGTTTAACTCTCCATCTTTTCGACTCACCCTTTAACTGATGGAAC11.1764705882352942No Hit
ACCCTCTGTCGCACTCATAGCTACAGCTCTAACTCGATTATTTCCTAATAA11.1764705882352942No Hit
GCTGTATTGCGCGCCATTTACTTGCTAGGTTCAGGGGCTGTCTCTTATACA11.1764705882352942No Hit
AAACTCAAGGGCCACATCTGAGAGTGAGACAATGTATTGTGTCTATGTCTG11.1764705882352942No Hit
GAAGCGCGCGACCCACACCCGGCCATCTGGGCGAACGCCATGCCCCGATGA11.1764705882352942No Hit
ATCTTAGGATAATTAGAGGGTGTTTAGTACGAAAGGATTGGCGTCTTTGGC11.1764705882352942No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCG11.1764705882352942No Hit
CTTCACATGTTTCTAGCGATTCACATGGCATCATCAAATGATACAAGTCCT11.1764705882352942No Hit
GATGATGGCCCCATTGTGTTAATATTAGCTCCCACAAGAGAACTAGCTGTT11.1764705882352942No Hit
CCCGTACCCTAAACCGACACTGGTGGATAGGTAGAGTATACCGAGGCGATC11.1764705882352942No Hit
CTCCCAGCAGAAATTCAGTTGATAGTGAGAACTCAGTGAATATACAATCAG11.1764705882352942No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC11.1764705882352942No Hit
ACTTTAAATAGAGGGATGGAGAAGAACCTTCTTTCTGCATTTTATCCTCCA11.1764705882352942No Hit
GTTCTTAGGTTACTTAATTTCATATGACCTCTCAAGCCACTACTTCAGAAT11.1764705882352942No Hit
AAATATTCTTGCATGGAATAATGGAATAAGATCTGTCTCTTATACACATCT11.1764705882352942No Hit
CTTTATGAGACTGAATGGAAACCTTCTTTCTTCGATCAGAATACTGTCTCT11.1764705882352942No Hit
TATCAACGCAGCGTACGGGATTGGATTTTATCTTAGGATAATTAGAGGGTG11.1764705882352942No Hit
GATACCACTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG11.1764705882352942No Hit
AAGAAACACAGCTAGAGTTATCGTATTTAACGTAGTATCTGTCTCTTATAC11.1764705882352942No Hit
TACATATCTTCTTAGATACAGAAGATTGTGATACTATCATCTGTCTCTTAT11.1764705882352942No Hit
GATTTCACACTGCCGAGAAAAGCCTCTAGATAGATAACCTGTCTCTTATAC11.1764705882352942No Hit
CTGTAATTGTACGTACTTGTAAAGAACTCAAACGTAACAATGGGACGATAC11.1764705882352942No Hit
GTTACAGCTTGATGCAGTTTCAAAGCAGCACATCCAAACTCATTTTCCAAT11.1764705882352942No Hit
ATCGTTACTTATGCCTACATTTTCTTTTCTGTCCGCTCCACAATCTGTCTC11.1764705882352942No Hit
GTCCTATTGACTATGCCAATCTTTTATATGACATTGCGGCTGTCTCTTATA11.1764705882352942No Hit
GAGTAAAATCAGCCTACGAATGGTTTAACGGATTTATGGGACTAGTTTTAG11.1764705882352942No Hit
AGACAATGACCATGGCTTCTTGGTCTTCAACTGGATCATGGCGTTCAAGTT11.1764705882352942No Hit
GATAGACAGTGAAAACGCTCAACCATTGGATGATATGAAGTTAATGAATGA11.1764705882352942No Hit
CCCCCAACTAAAATCAAAAGCCAACATCGAGGTAATAAACTTCCCCTAAAA11.1764705882352942No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGAT11.1764705882352942No Hit
GATCGGGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCG11.1764705882352942No Hit
GGACTGTATAGTGGATGCGAGTATCTTTATTGTTGTATGTCTGTCTCTTAT11.1764705882352942No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATA11.1764705882352942No Hit
CTCCAGAAAAGATTAAACTTTTAAGTCTTGTTTCCAAAAGCAAAAGATAGA11.1764705882352942No Hit
ACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGCTGTCTCTTATACA11.1764705882352942No Hit
TGGTAACTATAACCATCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCC11.1764705882352942No Hit
CCTTCAAGGTGGGGGTAAATATCAAAGCGAAGAAAAGAGAACTAACACTGT11.1764705882352942No Hit
AGATGAAGAAGCTAAGAAGAGTCCAGGGCTTGGAAGGACAGAATCAGCTGA11.1764705882352942No Hit
TCATTTATGGGGTCTCAGAACCCAATTATTCATGATTGACCAAATCATCTG11.1764705882352942No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG11.1764705882352942No Hit
TAACAATGGAGGCAATGAAGATGAAGCTTTACGTGGTGGTTTTGGTGGCAG11.1764705882352942No Hit
CTTTTGAAGTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTT11.1764705882352942No Hit
AACACAGGGTTTTGTCTGAATCTTTTTGGTTTATTTCCGAGAGAATTTGTG11.1764705882352942No Hit
CTTAAAATCACATATTCAGCTCATACAGATCTGACTGTCAAATTTCAGAGT11.1764705882352942No Hit
ATGTGGAAGTGTCACTCATAGCATGATACAGGGGAGAAATCGTATCTGATA11.1764705882352942No Hit
ACATAAGGTAGTCAAAAACTAAAGTAATAACAGCTGTCTCTTATACACATC11.1764705882352942No Hit
ATACTGAAGTTAGCAAACTTCTCGTATGCTGGAAACCATGTGTAGAATCCA11.1764705882352942No Hit
CGCCTAAAGAATGGCTTGTAAACCCATCAGAATCTACACGTACCTTATTCT11.1764705882352942No Hit
ATCATTTTGTCTTGGTTATACTTACGAGCCAACATCATCAATGAAGGCTCA11.1764705882352942No Hit
ACACAAATTACCCCGTTGACACTGTATATAATTTCTGATATGATTGCGCGT11.1764705882352942No Hit
CTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAATTTGTACGAA11.1764705882352942No Hit
CAATAGGTGAATAGTCATTCAATCCCGTACTCTGCGTTGATACTGTCTCTT11.1764705882352942No Hit
GAATGGAATGGAATGGAACGGAATGGAATGGAATGGAATCAATCTGAGTGC11.1764705882352942No Hit
GAATGGAATGGAATCAACCCGAGTGCAATGGAATGGAGTGGAATGGAATGG11.1764705882352942No Hit
CACTTCTTCTTCCTGCAGTTGACAGCTCTTGGGTGAAGACGAGCATAGCAC11.1764705882352942No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAATGATATGCTTA11.1764705882352942No Hit
GTGGATAGGGGTGAAAGGCCAATCAAACACCTGTCTCTTATACACATCTCC11.1764705882352942No Hit
GGTATCAACGCAGAGTACGGGGCACTCAAAAAATAAAAGCTGTCTCTTATA11.1764705882352942No Hit
GTACGTAGACGTGTATATTTATATGTACGTGAGACGATCACGGTGCTCTCA11.1764705882352942No Hit
ACGATTGACCTTTCCAAGTCATTCCTCTAAATTATAATCAGTCTGTCTCTT11.1764705882352942No Hit
CATGAGAATAGAGTCAATAGGGAAACCCATTTGTTGAACCAATAAAGATCT11.1764705882352942No Hit
GATATAGCTGGTTTTCCGCGAAATCTATTTTCAGTAGCTGTCTCTTATACA11.1764705882352942No Hit
TCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATC11.1764705882352942No Hit
GTCTTTTCATTGTGTGAGGCTCCTCAGGCATCAATGTACATAGTTCAGCAT11.1764705882352942No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTA11.1764705882352942No Hit
ATTGGATTTTATCTTAGGATAATTAGAGGGTGTTTAGTCTGTCTCTTATAC11.1764705882352942No Hit
CCTTTAATGGTGGATCTAGGTGTTGCTTTAGTGTTGTTGATCTTAATGTGA11.1764705882352942No Hit
GAGCATGTTTAATTGTTCATTACACAAGCAAAATGAAGAACTCTGTGACCT11.1764705882352942No Hit
CTCATGAAGATGATGATAATGATGATAATAACAACAGAGGATGAGACAAAG11.1764705882352942No Hit
TCTGTGAGGTGGTTTGAGTGTAGAGAATGTATGTCTTTGCTGTAGCAGATT11.1764705882352942No Hit
CCCTCAGAACTGACAAAATCAAAATCGAAAATGGCATCTGTCTCTTATACA11.1764705882352942No Hit
TTTAGGGACCTTAGCTGATGGTCTGGGTTGTTTCTGTCTCTTATACACATC11.1764705882352942No Hit
TCGCCTAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGCTCTATTC11.1764705882352942No Hit
TTATCACTGTGGACAATGAGACTATCTGAGTCCTCTTTTCTTCCTGCTGAT11.1764705882352942No Hit
GCTAAAGCTAATCCTGTGGTGAAGGCTGATAAGGTTGATAGGATATGAATC11.1764705882352942No Hit
AACTTAATGATCTTCTCCGAATCAGGAAGAGACTTAGCCACACTCTCTCTC11.1764705882352942No Hit
GTAATGTAAGCATGAGACTTTTTGGTAGTACCGGTGAACCAGATGGCCTGT11.1764705882352942No Hit
CCTTAAAACAACAAAGCTTTCGAATAAGCACTGTCTCTTATACACATCTCC11.1764705882352942No Hit
GTCTTATACAATTCAGCCATTGCTTCAACAGTTTTGGGGATACAAAATCTG11.1764705882352942No Hit
GTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAG11.1764705882352942No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTG11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCT50.045.023
GTTGACA50.045.018
GGTGAAG50.045.032
AGCTCTT50.045.024
CAGTTGA50.045.016
GACAGCT50.045.021
CCTGCAG50.045.012
CTCTTGG50.045.026
TGACAGC50.045.020
GAAGACG50.045.035
ACTTCTT50.045.02
GAGCATA50.045.041
CTTGGGT50.045.028
TTGGGTG50.045.029
TTCCTGC50.045.010
GCAGTTG50.045.015
ATAGCAC50.045.045
AAGACGA50.045.036
ACGAGCA50.045.039
TTCTTCT50.045.04
Produced by FastQC (version 0.11.2)