FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_86.3410000000a4e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_86.3410000000a4e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTAGACGTAAAGGGCATGATAGGGTTATGAAATCGATTGATACTGCTAT11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC11.7543859649122806No Hit
ATACGAGCCTGTATGGGCAATTGGAACTGGTAAAGTTGCATCTCCTCAGCA11.7543859649122806No Hit
GAGCCGTTCTCAGACGGAATCGAACTTTCCATCATCGGCGATTTCAATATC11.7543859649122806No Hit
ATTGTAATGCTTTCAAACACAAATCATTGTTACTTTAGAACCTGTCTCTTA11.7543859649122806No Hit
CTCAAGGTCTTGACGGTCTCGGTGACAGATGCAAGAAGTACTACGACTGTC11.7543859649122806No Hit
ATTTATCGTTACTCATGTCAGCATTCTCACTTCTGATATCTCCAGGTCTTG11.7543859649122806No Hit
CTCCAGTGGAAGATACATTGCAATAGTTGAGCAATTACTAAATGTCCTCTT11.7543859649122806No Hit
GGTCTGGGTTAGAACCTCAAAGCCACCAGGGTGGTATTTCAAGGACGGCTC11.7543859649122806No Hit
CTACAGTAGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATA11.7543859649122806No Hit
CCTTTGACTGGACCAGGTTCCCTAGTCACATTTCCACCCTTGGCACGGACG11.7543859649122806No Hit
ATGACTTGGTTCAGTTTTCTGAAGTAAACGGTGCTAGTAATGGTGACGAAA11.7543859649122806No Hit
ACTCAGTTGAATTTACCAATCTCCATTTTCTTCTTAATTGCTCGTTGTCCA11.7543859649122806No Hit
ACAAACATGGGCGCGTCCGCTGAAGGTGCAGAAATGATGACCTTCTTAGCA11.7543859649122806No Hit
AGATAAAAGACATCAAACATAACCAACATGAAATCAACTAGAACTCAAAAC11.7543859649122806No Hit
CTTGGGGACGACATCACCACGGTACATCAAACAGCAAGCCATGTACTTTCC11.7543859649122806No Hit
GTTGTGAGATTAGGTATCTCAAATGTTATATCGATGTTATTGGATAGATTA11.7543859649122806No Hit
TAAAGAAGCATTGTAAAGGTAGATTAGCCTTCCTTTTCATCGTTAGTATAC11.7543859649122806No Hit
GGATTTGATATTCCGATCGTCGGAGATCTAGAAACGAGAGAAGATCAAGCC11.7543859649122806No Hit
TGATATGACAGTGACTATTCCAAAAACCTCTCCACCACCCACGATGCTAAG11.7543859649122806No Hit
GACCAATATTAAGCCCCACAGTAGGAACAATTCGATCATGAGGAAGACCTT11.7543859649122806No Hit
GCTTAAGATGCCGGTATAAACGTATAATAGACATTTTGCTTCCCAATTCGT11.7543859649122806No Hit
ATCGTATACAGAAGTAGTTAAACAGTCATTGAATCACCTGATGAATTTAGA11.7543859649122806No Hit
TCATAGAACTGTTCTTCATCTTCTCCAGCATATGGTTAGGGAGATTCTACT11.7543859649122806No Hit
TTGTAATTCAGATTCCGGAATCCTTCATCATCTCTATCTGAGTTTAGTTTT11.7543859649122806No Hit
GGGAATACAGTTTCTCCAACTTTCGTCACATTGGATAGATAAATAAGAATT11.7543859649122806No Hit
GGACCAAATATCTGCAACGGCACCATTGTAACCCTTGTGACTGAGAACCTC11.7543859649122806No Hit
TCATAAACTCAACACAGTAAAACTAGTAGCAACACAATGATTTTTGGTTCG11.7543859649122806No Hit
CATGTAGACGGAATAACCCAAACATCCATAGTAAAAATCAGCTAAGGTCCT11.7543859649122806No Hit
TCTCAGCACTTAGAGACTGTTATCAGGCAGAAAATCCCCAGTATTGTTGCT11.7543859649122806No Hit
TCCCTGAGCCGCTCTCAGTGTTTGTTGACACCTACAAGACCGGGACCATCC11.7543859649122806No Hit
GGAGGAGGAGGAGAAATCAGAGAAGTTGTAAGAGGAAGAAGTAGCCTCAGA11.7543859649122806No Hit
CAGCAGGGGCTGACCAAGAAGACATGAACATCCATGTCCCAACTAAACAGA11.7543859649122806No Hit
TATCAACGCAGAGTACGGGGGTGGGGCGGACGCTGGGCCAACTGACTGGTA11.7543859649122806No Hit
GATTCATCTGATTGTTGAAGAAGGGAAAATAGATGACTTAATGGACATAGA11.7543859649122806No Hit
ATATAAGAGAATTAGTGGAGAAAGAACCAACGCAAAGTCATTTGGATTACA11.7543859649122806No Hit
GTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAG11.7543859649122806No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCG11.7543859649122806No Hit
CTTATTACAGAGCACAATGAGAAACAAGTGTTATTCAAATGCTAGACTTGA11.7543859649122806No Hit
TTACATTGCAGGATTAAGAATGCTTGTTGGATGTGAGGTAACATCAGTCTC11.7543859649122806No Hit
CAACCGAACAGGGTACAATGATAAGATAAAGATAGCCATTGATATTGCCGC11.7543859649122806No Hit
TTTGAAACTCTGCATCAACTTGATAACTTGCAGATTTGGAACACTCTCAAT11.7543859649122806No Hit
ACATGATACTGTATGGTGTGTCTCCACCAAATTTCTTTTGGTCAACATCAC11.7543859649122806No Hit
TGGTAATACTGGCGTTGTTCACCATGAAAATAAAAGTACGATGGATAAGAT11.7543859649122806No Hit
ATCGTAAAAGGCGTCGTCATAGCAAGGCGTAATGCTGGTCTCAACTCAACA11.7543859649122806No Hit
CGTCCAAACCCACTGCGTTTGACACCACCCCATGGGGCTTGGGTAAAGCAA11.7543859649122806No Hit
ATACTAGAGAGCTTCAAAGCTCTCCTTCGTAGATTCGTGTATTGTACAGAT11.7543859649122806No Hit
GTTGTGTCATGTATCAAAGAAGCAGCAGATGCTACATCCCCCTGTCTCTTA11.7543859649122806No Hit
ATTGAAAGGGTCATCCAAGCAAGGTCACAATTTGGTTATAAAATGGTTGTT11.7543859649122806No Hit
CCCCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCC11.7543859649122806No Hit
TGCAGGTTGGATACGCCAATCATTTTTATCGAAGCGCGCATAAATTTGAGC11.7543859649122806No Hit
GAAAACAGAGGAGGAATCTGCAGAGTGATTGTTTGATAGAAGTTTGATGAC11.7543859649122806No Hit
CTGTTGTATCTTTATAGAATGTCGAAAATGTAGTTCCAACTCCTGTCTCTT11.7543859649122806No Hit
GAAGAGAAGTTTCTTAGGTTCGAAGTTTTCAAGGATAATCTAAAGCCTGTC11.7543859649122806No Hit
CTACATAACCGAAGAGAATGATCTGCAAGGGCTGATTCCGAACAATGCCTA11.7543859649122806No Hit
ATATTAGTCATATCAGTCACCAAATCGTTGTACCTGTCTCTTATACACATC11.7543859649122806No Hit
TAAGATGATTCTAGACACAAGACAACCCCATTGGTTGAGTCCTGTCTCTTA11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCAA50.045.031
AGCCATG50.045.037
CAGCAAG50.045.032
GCAAGCC50.045.034
GTACTTT50.045.043
AAACAGC50.045.029
ACCACGG50.045.016
CCACGGT50.045.017
CCATGTA50.045.039
GTACATC50.045.022
CATCACC50.045.012
CATCAAA50.045.025
TACATCA50.045.023
ACATCAC50.045.011
ACATCAA50.045.024
ACTTTCC50.045.045
CTTGGGG50.045.01
GACATCA50.045.010
TTGGGGA50.045.02
TGGGGAC50.045.03
Produced by FastQC (version 0.11.2)