##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_2_85.3410000000a4c7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.285714285714285 0.0 0.0 0.0 0.0 0.0 2 34.666666666666664 0.0 0.0 0.0 0.0 0.0 3 34.23809523809524 0.0 0.0 0.0 0.0 0.0 4 34.333333333333336 0.0 0.0 0.0 0.0 0.0 5 34.523809523809526 0.0 0.0 0.0 0.0 0.0 6 35.92857142857143 0.0 0.0 0.0 0.0 0.0 7 34.833333333333336 0.0 0.0 0.0 0.0 0.0 8 34.904761904761905 0.0 0.0 0.0 0.0 0.0 9 35.833333333333336 0.0 0.0 0.0 0.0 0.0 10 35.23809523809524 0.0 0.0 0.0 0.0 0.0 11 35.45238095238095 0.0 0.0 0.0 0.0 0.0 12 35.142857142857146 0.0 0.0 0.0 0.0 0.0 13 34.26190476190476 0.0 0.0 0.0 0.0 0.0 14 34.23809523809524 0.0 0.0 0.0 0.0 0.0 15 33.76190476190476 0.0 0.0 0.0 0.0 0.0 16 32.76190476190476 0.0 0.0 0.0 0.0 0.0 17 34.857142857142854 0.0 0.0 0.0 0.0 0.0 18 34.023809523809526 0.0 0.0 0.0 0.0 0.0 19 34.80952380952381 0.0 0.0 0.0 0.0 0.0 20 33.57142857142857 0.0 0.0 0.0 0.0 0.0 21 35.80952380952381 0.0 0.0 0.0 0.0 0.0 22 34.404761904761905 0.0 0.0 0.0 0.0 0.0 23 33.904761904761905 0.0 0.0 0.0 0.0 0.0 24 35.5 0.0 0.0 0.0 0.0 0.0 25 35.30952380952381 0.0 0.0 0.0 0.0 0.0 26 36.42857142857143 0.0 0.0 0.0 0.0 0.0 27 36.26190476190476 0.0 0.0 0.0 0.0 0.0 28 33.404761904761905 0.0 0.0 0.0 0.0 0.0 29 34.833333333333336 0.0 0.0 0.0 0.0 0.0 30 33.976190476190474 0.0 0.0 0.0 0.0 0.0 31 32.976190476190474 0.0 0.0 0.0 0.0 0.0 32 33.523809523809526 0.0 0.0 0.0 0.0 0.0 33 33.26190476190476 0.0 0.0 0.0 0.0 0.0 34 33.642857142857146 0.0 0.0 0.0 0.0 0.0 35 32.73809523809524 0.0 0.0 0.0 0.0 0.0 36 33.404761904761905 0.0 0.0 0.0 0.0 0.0 37 34.785714285714285 0.0 0.0 0.0 0.0 0.0 38 34.11904761904762 0.0 0.0 0.0 0.0 0.0 39 33.857142857142854 0.0 0.0 0.0 0.0 0.0 40 32.476190476190474 0.0 0.0 0.0 0.0 0.0 41 33.07142857142857 0.0 0.0 0.0 0.0 0.0 42 35.214285714285715 0.0 0.0 0.0 0.0 0.0 43 34.07142857142857 0.0 0.0 0.0 0.0 0.0 44 34.714285714285715 0.0 0.0 0.0 0.0 0.0 45 34.214285714285715 0.0 0.0 0.0 0.0 0.0 46 34.26190476190476 0.0 0.0 0.0 0.0 0.0 47 35.11904761904762 0.0 0.0 0.0 0.0 0.0 48 35.04761904761905 0.0 0.0 0.0 0.0 0.0 49 35.904761904761905 0.0 0.0 0.0 0.0 0.0 50 34.26190476190476 0.0 0.0 0.0 0.0 0.0 51 33.80952380952381 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1203 1 6.25 1203 2 9.0 1203 3 7.75 1203 4 4.25 1203 5 -11.0 1203 6 -7.25 1203 7 -7.25 1203 8 1.0 1203 9 -8.75 1203 10 0.75 1203 11 -4.0 1203 12 -17.25 1203 13 -11.0 1203 14 -1.0 1203 15 7.25 1203 16 -3.5 1203 17 -17.75 1203 18 -17.75 1203 19 -3.0 1203 20 2.75 1203 21 -1.5 1203 22 -4.0 1203 23 -13.0 1203 24 -2.25 1203 25 -2.25 1203 26 2.5 1203 27 -16.5 1203 28 -10.25 1203 29 -3.25 1203 30 2.5 1203 31 12.5 1203 32 -9.75 1203 33 8.0 1203 34 2.25 1203 35 8.0 1203 36 -16.0 1203 37 -6.25 1203 38 4.25 1203 39 3.0 1203 40 1.75 1203 41 -17.25 1203 42 0.0 1203 43 -16.5 1203 44 2.75 1203 45 1.5 1203 46 3.0 1203 47 0.0 1203 48 -2.25 1203 49 4.25 1203 50 3.75 1203 51 -6.75 1211 1 6.25 1211 2 -16.0 1211 3 -17.25 1211 4 -6.75 1211 5 8.0 1211 6 3.75 1211 7 3.75 1211 8 1.0 1211 9 2.25 1211 10 8.75 1211 11 4.0 1211 12 1.75 1211 13 -11.0 1211 14 -15.0 1211 15 -17.75 1211 16 2.5 1211 17 1.25 1211 18 7.25 1211 19 3.0 1211 20 2.75 1211 21 -1.5 1211 22 1.0 1211 23 -13.0 1211 24 -7.25 1211 25 -7.25 1211 26 -3.5 1211 27 -2.5 1211 28 3.75 1211 29 -3.25 1211 30 -3.5 1211 31 -12.5 1211 32 4.25 1211 33 -11.0 1211 34 2.25 1211 35 -11.0 1211 36 9.0 1211 37 -6.25 1211 38 -6.75 1211 39 -3.0 1211 40 -17.25 1211 41 7.75 1211 42 0.0 1211 43 8.5 1211 44 2.75 1211 45 -4.5 1211 46 -3.0 1211 47 0.0 1211 48 3.75 1211 49 -1.75 1211 50 -7.25 1211 51 4.25 2111 1 6.25 2111 2 9.0 2111 3 7.75 2111 4 4.25 2111 5 14.0 2111 6 5.75 2111 7 5.75 2111 8 3.0 2111 9 4.25 2111 10 8.75 2111 11 4.0 2111 12 7.75 2111 13 14.0 2111 14 12.0 2111 15 9.25 2111 16 4.5 2111 17 9.25 2111 18 9.25 2111 19 3.0 2111 20 10.75 2111 21 4.5 2111 22 7.0 2111 23 14.0 2111 24 5.75 2111 25 5.75 2111 26 4.5 2111 27 10.5 2111 28 16.75 2111 29 9.75 2111 30 4.5 2111 31 12.5 2111 32 15.25 2111 33 14.0 2111 34 4.25 2111 35 14.0 2111 36 9.0 2111 37 18.75 2111 38 4.25 2111 39 3.0 2111 40 7.75 2111 41 7.75 2111 42 0.0 2111 43 10.5 2111 44 10.75 2111 45 1.5 2111 46 3.0 2111 47 0.0 2111 48 5.75 2111 49 4.25 2111 50 5.75 2111 51 4.25 2212 1 -18.75 2212 2 -2.0 2212 3 1.75 2212 4 -1.75 2212 5 -11.0 2212 6 -2.25 2212 7 -2.25 2212 8 -5.0 2212 9 2.25 2212 10 -18.25 2212 11 -4.0 2212 12 7.75 2212 13 8.0 2212 14 4.0 2212 15 1.25 2212 16 -3.5 2212 17 7.25 2212 18 1.25 2212 19 -3.0 2212 20 -16.25 2212 21 -1.5 2212 22 -4.0 2212 23 12.0 2212 24 3.75 2212 25 3.75 2212 26 -3.5 2212 27 8.5 2212 28 -10.25 2212 29 -3.25 2212 30 -3.5 2212 31 -12.5 2212 32 -9.75 2212 33 -11.0 2212 34 -8.75 2212 35 -11.0 2212 36 -2.0 2212 37 -6.25 2212 38 -1.75 2212 39 -3.0 2212 40 7.75 2212 41 1.75 2212 42 0.0 2212 43 -2.5 2212 44 -16.25 2212 45 1.5 2212 46 -3.0 2212 47 0.0 2212 48 -7.25 2212 49 -6.75 2212 50 -2.25 2212 51 -1.75 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 0.0 26 0.0 27 1.0 28 3.0 29 4.0 30 1.0 31 6.0 32 0.0 33 1.0 34 2.0 35 0.0 36 3.0 37 4.0 38 7.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.61904761904761 28.57142857142857 7.142857142857142 16.666666666666664 2 7.142857142857142 19.047619047619047 45.23809523809524 28.57142857142857 3 16.666666666666664 23.809523809523807 30.952380952380953 28.57142857142857 4 19.047619047619047 23.809523809523807 30.952380952380953 26.190476190476193 5 19.047619047619047 33.33333333333333 35.714285714285715 11.904761904761903 6 23.809523809523807 47.61904761904761 14.285714285714285 14.285714285714285 7 33.33333333333333 30.952380952380953 14.285714285714285 21.428571428571427 8 28.57142857142857 40.476190476190474 14.285714285714285 16.666666666666664 9 23.809523809523807 23.809523809523807 19.047619047619047 33.33333333333333 10 23.809523809523807 30.952380952380953 19.047619047619047 26.190476190476193 11 42.857142857142854 19.047619047619047 16.666666666666664 21.428571428571427 12 14.285714285714285 28.57142857142857 35.714285714285715 21.428571428571427 13 23.809523809523807 28.57142857142857 19.047619047619047 28.57142857142857 14 23.809523809523807 28.57142857142857 23.809523809523807 23.809523809523807 15 19.047619047619047 23.809523809523807 11.904761904761903 45.23809523809524 16 26.190476190476193 30.952380952380953 16.666666666666664 26.190476190476193 17 30.952380952380953 28.57142857142857 19.047619047619047 21.428571428571427 18 19.047619047619047 26.190476190476193 28.57142857142857 26.190476190476193 19 28.57142857142857 26.190476190476193 21.428571428571427 23.809523809523807 20 26.190476190476193 35.714285714285715 21.428571428571427 16.666666666666664 21 28.57142857142857 23.809523809523807 21.428571428571427 26.190476190476193 22 23.809523809523807 40.476190476190474 16.666666666666664 19.047619047619047 23 21.428571428571427 30.952380952380953 30.952380952380953 16.666666666666664 24 33.33333333333333 30.952380952380953 16.666666666666664 19.047619047619047 25 23.809523809523807 28.57142857142857 30.952380952380953 16.666666666666664 26 14.285714285714285 33.33333333333333 30.952380952380953 21.428571428571427 27 26.190476190476193 19.047619047619047 21.428571428571427 33.33333333333333 28 35.714285714285715 14.285714285714285 33.33333333333333 16.666666666666664 29 21.428571428571427 23.809523809523807 19.047619047619047 35.714285714285715 30 14.285714285714285 33.33333333333333 23.809523809523807 28.57142857142857 31 33.33333333333333 21.428571428571427 21.428571428571427 23.809523809523807 32 16.666666666666664 30.952380952380953 30.952380952380953 21.428571428571427 33 23.809523809523807 26.190476190476193 23.809523809523807 26.190476190476193 34 21.428571428571427 28.57142857142857 28.57142857142857 21.428571428571427 35 19.047619047619047 35.714285714285715 23.809523809523807 21.428571428571427 36 21.428571428571427 26.190476190476193 28.57142857142857 23.809523809523807 37 28.57142857142857 35.714285714285715 14.285714285714285 21.428571428571427 38 19.047619047619047 30.952380952380953 38.095238095238095 11.904761904761903 39 26.190476190476193 19.047619047619047 19.047619047619047 35.714285714285715 40 33.33333333333333 16.666666666666664 21.428571428571427 28.57142857142857 41 19.047619047619047 26.190476190476193 28.57142857142857 26.190476190476193 42 16.666666666666664 26.190476190476193 28.57142857142857 28.57142857142857 43 26.190476190476193 33.33333333333333 19.047619047619047 21.428571428571427 44 28.57142857142857 26.190476190476193 28.57142857142857 16.666666666666664 45 19.047619047619047 26.190476190476193 38.095238095238095 16.666666666666664 46 21.428571428571427 21.428571428571427 28.57142857142857 28.57142857142857 47 19.047619047619047 33.33333333333333 14.285714285714285 33.33333333333333 48 19.047619047619047 30.952380952380953 35.714285714285715 14.285714285714285 49 28.57142857142857 33.33333333333333 21.428571428571427 16.666666666666664 50 26.190476190476193 21.428571428571427 26.190476190476193 26.190476190476193 51 30.952380952380953 28.57142857142857 16.666666666666664 23.809523809523807 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.0 25 0.5 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 1.5 33 3.0 34 2.5 35 2.0 36 1.0 37 0.0 38 2.0 39 4.0 40 4.5 41 5.0 42 3.5 43 2.0 44 1.5 45 1.0 46 1.0 47 1.0 48 1.5 49 2.0 50 3.0 51 4.0 52 4.0 53 4.0 54 3.5 55 3.0 56 4.0 57 5.0 58 3.0 59 1.0 60 1.5 61 2.0 62 1.5 63 1.0 64 0.5 65 0.0 66 0.5 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 42.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCTTACTGGTCTTAGTTGTGATGACTCTTCATCTGAAACTCAGGTGTATG 1 2.380952380952381 No Hit CTACCTGGAGCCCACTTGCATCTGGTGTGCTCACTGGTAAATACCTGTCTC 1 2.380952380952381 No Hit GCATAATTGAAAACCATAATAAAAAGTCTCAGAGATATCCAAAAAGGAGGG 1 2.380952380952381 No Hit GGTCCAACTCCTCGACCTTCAAACTTCGAGGAGAATTGCGCCATGGCATCC 1 2.380952380952381 No Hit ATTTTGTGGAGCCGCAACAACAAAAAGAATATGACCAAAGGAGGTACTATG 1 2.380952380952381 No Hit GTGCTCAGTGGTGGAACCACTATGTTCTCAGGTATCGCTGACCGTATGAGC 1 2.380952380952381 No Hit CTTGTGGGAAGAATGATTCTAGCTCACTTATGTGACGAACCAAAAGGATTG 1 2.380952380952381 No Hit GTCCAACACGGGCACGAGCACCCGGGGGCTCATTCATTTGTCCATACACTA 1 2.380952380952381 No Hit GCTTGTGGCGGATCCGAGGAGGTGTGAGCCGAAGAAGTTTGGTGGTCGTGG 1 2.380952380952381 No Hit GCTGGAGATTCCGATATCGGGATGAACTTGCTTAAGCACCTTGAAGATGTA 1 2.380952380952381 No Hit AAAATAAACAGAAAACGATATGAGATCGAAAAAGGTCGGTCATATAGAGCT 1 2.380952380952381 No Hit GCCAAAGAAAGCATCACTCAGAAAATCTCAGAAGAAGTTTGTCAGTAATCC 1 2.380952380952381 No Hit CAGTAGTGATGAAGGGGTCGTTGACAGCGACGAGCTCAACATCGTCCCTCT 1 2.380952380952381 No Hit ACGCAGAGTACGGGAACTCTCTCATAATCTCTCTCCCTCACTCTAACACCA 1 2.380952380952381 No Hit ACCACAGAGATTCAGATGTAAAAACCCCACCGGTGAAATGACGGCGAAGGG 1 2.380952380952381 No Hit ACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACC 1 2.380952380952381 No Hit GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA 1 2.380952380952381 No Hit CTATGTGTGTATTAGCATATAATTCTTACAACAAAAGACAAATTTATGAAA 1 2.380952380952381 No Hit GTACAGAAGAGTCTCCCCTGTCTGGAATGAGTCCTTATGGACGGACAAAGC 1 2.380952380952381 No Hit GAGTGAGAGAATCATGGAGGAAGGAAGTATGTTCAGATCTCTATTAGCGCT 1 2.380952380952381 No Hit GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT 1 2.380952380952381 No Hit TAATTACCCAAAACCCTTGAATCCATTACTCTGTGTGAGCTTAAATCTCTT 1 2.380952380952381 No Hit TATGTACGCCAAACGTGCTTTCGTTCACTGGTATGTTGGTGAGGGTATGGA 1 2.380952380952381 No Hit GTACATAATAGCGGCGAAGGCGGTTCCGGCGAAGAAAGCCTTGTTGGAGAA 1 2.380952380952381 No Hit GAGATCGATGCTCTCTTGAGCGCAGATGAAGATTATGAAGAGAATGATGAT 1 2.380952380952381 No Hit CCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCTCAGAAG 1 2.380952380952381 No Hit ATCTTAAGAGTTTTACAAAAGATGTCTTGGAAGCATATGGTCCTCCCTCAG 1 2.380952380952381 No Hit GTTGAGGGAGCAGATCGAGGGTTAAAAGTCTGCTGGCTTTTTATATTTGGT 1 2.380952380952381 No Hit ATTAGACATATAAAAGGCAACAACAACTCCAGCTGTATAATCAGAAGGTAA 1 2.380952380952381 No Hit GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCA 1 2.380952380952381 No Hit GTCGTCGCACAGACAGCCAGGAGGTTGGCTTAGAAGCAGCCACCCTTCAAA 1 2.380952380952381 No Hit AAACGACTCCGTCGATAAGAGCTCTTGGGAGTCATCAGCCTGTTATCCCCG 1 2.380952380952381 No Hit CCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGGAGGGGAGTGAAATAG 1 2.380952380952381 No Hit GCTACGGAAGCTGCCGCCGTCGTCGACGGTGTTGTTGAGTCAGATTCGGAG 1 2.380952380952381 No Hit TTCATGTATTGAGCGTACCGATGAAACGACCCAAAACCTGTCTCTTATACA 1 2.380952380952381 No Hit ATCAAAAGGTTCTCCAGTTCCAATGTCTCTATCAAATCCGAGTTCCATATC 1 2.380952380952381 No Hit AGGATCTACTTCAGTACCAACAAACTGTTCGTCCCAATCAATAACCACATC 1 2.380952380952381 No Hit ATGCAACACAGAGTCAGTAGAGTTGTGGCGAAGATTGAGCTAGTGCCTCGG 1 2.380952380952381 No Hit GATCACAAGCATCCCTAATAGCAGCCAATCTTGTCCCTCTAGGAACAGAGG 1 2.380952380952381 No Hit GCTTAGGGACCTTAGCGTACGATCTGGGCTGTTTCCCTCCTGTCTCTTATA 1 2.380952380952381 No Hit ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGC 1 2.380952380952381 No Hit CTCCGAAACCGGATCCACTTCCGATCTGGATCCACATCTACTGGTCCAAGC 1 2.380952380952381 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 2.380952380952381 39 0.0 0.0 2.380952380952381 >>END_MODULE >>Kmer Content pass >>END_MODULE