FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_85.3410000000a4c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_85.3410000000a4c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTACTGGTCTTAGTTGTGATGACTCTTCATCTGAAACTCAGGTGTATG12.380952380952381No Hit
CTACCTGGAGCCCACTTGCATCTGGTGTGCTCACTGGTAAATACCTGTCTC12.380952380952381No Hit
GCATAATTGAAAACCATAATAAAAAGTCTCAGAGATATCCAAAAAGGAGGG12.380952380952381No Hit
GGTCCAACTCCTCGACCTTCAAACTTCGAGGAGAATTGCGCCATGGCATCC12.380952380952381No Hit
ATTTTGTGGAGCCGCAACAACAAAAAGAATATGACCAAAGGAGGTACTATG12.380952380952381No Hit
GTGCTCAGTGGTGGAACCACTATGTTCTCAGGTATCGCTGACCGTATGAGC12.380952380952381No Hit
CTTGTGGGAAGAATGATTCTAGCTCACTTATGTGACGAACCAAAAGGATTG12.380952380952381No Hit
GTCCAACACGGGCACGAGCACCCGGGGGCTCATTCATTTGTCCATACACTA12.380952380952381No Hit
GCTTGTGGCGGATCCGAGGAGGTGTGAGCCGAAGAAGTTTGGTGGTCGTGG12.380952380952381No Hit
GCTGGAGATTCCGATATCGGGATGAACTTGCTTAAGCACCTTGAAGATGTA12.380952380952381No Hit
AAAATAAACAGAAAACGATATGAGATCGAAAAAGGTCGGTCATATAGAGCT12.380952380952381No Hit
GCCAAAGAAAGCATCACTCAGAAAATCTCAGAAGAAGTTTGTCAGTAATCC12.380952380952381No Hit
CAGTAGTGATGAAGGGGTCGTTGACAGCGACGAGCTCAACATCGTCCCTCT12.380952380952381No Hit
ACGCAGAGTACGGGAACTCTCTCATAATCTCTCTCCCTCACTCTAACACCA12.380952380952381No Hit
ACCACAGAGATTCAGATGTAAAAACCCCACCGGTGAAATGACGGCGAAGGG12.380952380952381No Hit
ACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACC12.380952380952381No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA12.380952380952381No Hit
CTATGTGTGTATTAGCATATAATTCTTACAACAAAAGACAAATTTATGAAA12.380952380952381No Hit
GTACAGAAGAGTCTCCCCTGTCTGGAATGAGTCCTTATGGACGGACAAAGC12.380952380952381No Hit
GAGTGAGAGAATCATGGAGGAAGGAAGTATGTTCAGATCTCTATTAGCGCT12.380952380952381No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT12.380952380952381No Hit
TAATTACCCAAAACCCTTGAATCCATTACTCTGTGTGAGCTTAAATCTCTT12.380952380952381No Hit
TATGTACGCCAAACGTGCTTTCGTTCACTGGTATGTTGGTGAGGGTATGGA12.380952380952381No Hit
GTACATAATAGCGGCGAAGGCGGTTCCGGCGAAGAAAGCCTTGTTGGAGAA12.380952380952381No Hit
GAGATCGATGCTCTCTTGAGCGCAGATGAAGATTATGAAGAGAATGATGAT12.380952380952381No Hit
CCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCTCAGAAG12.380952380952381No Hit
ATCTTAAGAGTTTTACAAAAGATGTCTTGGAAGCATATGGTCCTCCCTCAG12.380952380952381No Hit
GTTGAGGGAGCAGATCGAGGGTTAAAAGTCTGCTGGCTTTTTATATTTGGT12.380952380952381No Hit
ATTAGACATATAAAAGGCAACAACAACTCCAGCTGTATAATCAGAAGGTAA12.380952380952381No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCA12.380952380952381No Hit
GTCGTCGCACAGACAGCCAGGAGGTTGGCTTAGAAGCAGCCACCCTTCAAA12.380952380952381No Hit
AAACGACTCCGTCGATAAGAGCTCTTGGGAGTCATCAGCCTGTTATCCCCG12.380952380952381No Hit
CCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGGAGGGGAGTGAAATAG12.380952380952381No Hit
GCTACGGAAGCTGCCGCCGTCGTCGACGGTGTTGTTGAGTCAGATTCGGAG12.380952380952381No Hit
TTCATGTATTGAGCGTACCGATGAAACGACCCAAAACCTGTCTCTTATACA12.380952380952381No Hit
ATCAAAAGGTTCTCCAGTTCCAATGTCTCTATCAAATCCGAGTTCCATATC12.380952380952381No Hit
AGGATCTACTTCAGTACCAACAAACTGTTCGTCCCAATCAATAACCACATC12.380952380952381No Hit
ATGCAACACAGAGTCAGTAGAGTTGTGGCGAAGATTGAGCTAGTGCCTCGG12.380952380952381No Hit
GATCACAAGCATCCCTAATAGCAGCCAATCTTGTCCCTCTAGGAACAGAGG12.380952380952381No Hit
GCTTAGGGACCTTAGCGTACGATCTGGGCTGTTTCCCTCCTGTCTCTTATA12.380952380952381No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGC12.380952380952381No Hit
CTCCGAAACCGGATCCACTTCCGATCTGGATCCACATCTACTGGTCCAAGC12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)