##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_2_84.3410000000a4ba.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.0 0.0 0.0 0.0 0.0 0.0 2 38.0 0.0 0.0 0.0 0.0 0.0 3 38.0 0.0 0.0 0.0 0.0 0.0 4 38.0 0.0 0.0 0.0 0.0 0.0 5 36.8 0.0 0.0 0.0 0.0 0.0 6 39.6 0.0 0.0 0.0 0.0 0.0 7 40.0 0.0 0.0 0.0 0.0 0.0 8 38.4 0.0 0.0 0.0 0.0 0.0 9 39.6 0.0 0.0 0.0 0.0 0.0 10 40.0 0.0 0.0 0.0 0.0 0.0 11 40.0 0.0 0.0 0.0 0.0 0.0 12 40.0 0.0 0.0 0.0 0.0 0.0 13 40.0 0.0 0.0 0.0 0.0 0.0 14 40.0 0.0 0.0 0.0 0.0 0.0 15 40.0 0.0 0.0 0.0 0.0 0.0 16 40.0 0.0 0.0 0.0 0.0 0.0 17 40.0 0.0 0.0 0.0 0.0 0.0 18 40.0 0.0 0.0 0.0 0.0 0.0 19 40.0 0.0 0.0 0.0 0.0 0.0 20 39.6 0.0 0.0 0.0 0.0 0.0 21 40.0 0.0 0.0 0.0 0.0 0.0 22 40.0 0.0 0.0 0.0 0.0 0.0 23 40.0 0.0 0.0 0.0 0.0 0.0 24 39.6 0.0 0.0 0.0 0.0 0.0 25 38.0 0.0 0.0 0.0 0.0 0.0 26 34.6 0.0 0.0 0.0 0.0 0.0 27 37.4 0.0 0.0 0.0 0.0 0.0 28 34.6 0.0 0.0 0.0 0.0 0.0 29 34.6 0.0 0.0 0.0 0.0 0.0 30 37.4 0.0 0.0 0.0 0.0 0.0 31 34.2 0.0 0.0 0.0 0.0 0.0 32 34.6 0.0 0.0 0.0 0.0 0.0 33 34.6 0.0 0.0 0.0 0.0 0.0 34 34.6 0.0 0.0 0.0 0.0 0.0 35 34.2 0.0 0.0 0.0 0.0 0.0 36 37.0 0.0 0.0 0.0 0.0 0.0 37 37.0 0.0 0.0 0.0 0.0 0.0 38 34.6 0.0 0.0 0.0 0.0 0.0 39 34.2 0.0 0.0 0.0 0.0 0.0 40 34.6 0.0 0.0 0.0 0.0 0.0 41 34.6 0.0 0.0 0.0 0.0 0.0 42 37.4 0.0 0.0 0.0 0.0 0.0 43 37.0 0.0 0.0 0.0 0.0 0.0 44 34.6 0.0 0.0 0.0 0.0 0.0 45 34.6 0.0 0.0 0.0 0.0 0.0 46 34.6 0.0 0.0 0.0 0.0 0.0 47 34.6 0.0 0.0 0.0 0.0 0.0 48 34.2 0.0 0.0 0.0 0.0 0.0 49 34.6 0.0 0.0 0.0 0.0 0.0 50 34.6 0.0 0.0 0.0 0.0 0.0 51 37.4 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 27 1.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.0 20.0 0.0 40.0 2 20.0 20.0 20.0 40.0 3 0.0 40.0 60.0 0.0 4 0.0 40.0 20.0 40.0 5 0.0 40.0 40.0 20.0 6 40.0 60.0 0.0 0.0 7 20.0 80.0 0.0 0.0 8 0.0 40.0 40.0 20.0 9 0.0 80.0 20.0 0.0 10 0.0 20.0 80.0 0.0 11 20.0 20.0 40.0 20.0 12 0.0 20.0 60.0 20.0 13 20.0 40.0 20.0 20.0 14 0.0 20.0 60.0 20.0 15 40.0 40.0 20.0 0.0 16 0.0 40.0 40.0 20.0 17 60.0 20.0 20.0 0.0 18 0.0 60.0 40.0 0.0 19 20.0 20.0 40.0 20.0 20 20.0 20.0 60.0 0.0 21 20.0 40.0 20.0 20.0 22 20.0 60.0 0.0 20.0 23 40.0 40.0 20.0 0.0 24 20.0 60.0 0.0 20.0 25 40.0 20.0 40.0 0.0 26 60.0 0.0 20.0 20.0 27 40.0 20.0 40.0 0.0 28 20.0 20.0 60.0 0.0 29 40.0 0.0 0.0 60.0 30 60.0 20.0 20.0 0.0 31 20.0 20.0 40.0 20.0 32 20.0 40.0 40.0 0.0 33 20.0 80.0 0.0 0.0 34 40.0 20.0 0.0 40.0 35 0.0 20.0 80.0 0.0 36 20.0 0.0 40.0 40.0 37 20.0 20.0 20.0 40.0 38 0.0 60.0 20.0 20.0 39 40.0 60.0 0.0 0.0 40 40.0 0.0 40.0 20.0 41 0.0 100.0 0.0 0.0 42 0.0 40.0 20.0 40.0 43 40.0 20.0 20.0 20.0 44 40.0 20.0 0.0 40.0 45 0.0 20.0 40.0 40.0 46 20.0 40.0 40.0 0.0 47 0.0 20.0 60.0 20.0 48 40.0 20.0 0.0 40.0 49 20.0 40.0 0.0 40.0 50 40.0 40.0 20.0 0.0 51 20.0 20.0 40.0 20.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.0 25 0.5 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.5 35 1.0 36 0.5 37 0.0 38 1.0 39 2.0 40 1.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.5 55 1.0 56 0.5 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 5.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATCAAATATTTTTTATATTTCTGTTTGGGTGAGTCAAGGACACCTTGGGT 1 20.0 No Hit CTAATGGATTCCATGTGTCTGAGATCGTCGAAAGTTCTAGAATAAAAGCAC 1 20.0 No Hit GCAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCTTTAATACCCTTTCCTT 1 20.0 No Hit GCTTCAATATTTCCATGTTGAGGAGGATCGTAACTGGAACATGGTATCAAA 1 20.0 No Hit AGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG 1 20.0 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE