##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_2_81.3410000000a484.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.07692307692308 0.0 0.0 0.0 0.0 0.0 2 36.61538461538461 0.0 0.0 0.0 0.0 0.0 3 37.07692307692308 0.0 0.0 0.0 0.0 0.0 4 38.0 0.0 0.0 0.0 0.0 0.0 5 37.07692307692308 0.0 0.0 0.0 0.0 0.0 6 39.23076923076923 0.0 0.0 0.0 0.0 0.0 7 39.53846153846154 0.0 0.0 0.0 0.0 0.0 8 39.53846153846154 0.0 0.0 0.0 0.0 0.0 9 39.53846153846154 0.0 0.0 0.0 0.0 0.0 10 39.69230769230769 0.0 0.0 0.0 0.0 0.0 11 39.23076923076923 0.0 0.0 0.0 0.0 0.0 12 39.38461538461539 0.0 0.0 0.0 0.0 0.0 13 38.30769230769231 0.0 0.0 0.0 0.0 0.0 14 38.76923076923077 0.0 0.0 0.0 0.0 0.0 15 38.38461538461539 0.0 0.0 0.0 0.0 0.0 16 37.69230769230769 0.0 0.0 0.0 0.0 0.0 17 39.23076923076923 0.0 0.0 0.0 0.0 0.0 18 38.92307692307692 0.0 0.0 0.0 0.0 0.0 19 37.46153846153846 0.0 0.0 0.0 0.0 0.0 20 38.07692307692308 0.0 0.0 0.0 0.0 0.0 21 38.30769230769231 0.0 0.0 0.0 0.0 0.0 22 38.07692307692308 0.0 0.0 0.0 0.0 0.0 23 35.92307692307692 0.0 0.0 0.0 0.0 0.0 24 37.30769230769231 0.0 0.0 0.0 0.0 0.0 25 38.92307692307692 0.0 0.0 0.0 0.0 0.0 26 38.92307692307692 0.0 0.0 0.0 0.0 0.0 27 39.53846153846154 0.0 0.0 0.0 0.0 0.0 28 38.76923076923077 0.0 0.0 0.0 0.0 0.0 29 39.23076923076923 0.0 0.0 0.0 0.0 0.0 30 39.38461538461539 0.0 0.0 0.0 0.0 0.0 31 38.30769230769231 0.0 0.0 0.0 0.0 0.0 32 38.30769230769231 0.0 0.0 0.0 0.0 0.0 33 39.07692307692308 0.0 0.0 0.0 0.0 0.0 34 38.76923076923077 0.0 0.0 0.0 0.0 0.0 35 36.84615384615385 0.0 0.0 0.0 0.0 0.0 36 37.0 0.0 0.0 0.0 0.0 0.0 37 38.15384615384615 0.0 0.0 0.0 0.0 0.0 38 38.92307692307692 0.0 0.0 0.0 0.0 0.0 39 37.15384615384615 0.0 0.0 0.0 0.0 0.0 40 35.92307692307692 0.0 0.0 0.0 0.0 0.0 41 38.61538461538461 0.0 0.0 0.0 0.0 0.0 42 38.61538461538461 0.0 0.0 0.0 0.0 0.0 43 39.53846153846154 0.0 0.0 0.0 0.0 0.0 44 38.46153846153846 0.0 0.0 0.0 0.0 0.0 45 38.46153846153846 0.0 0.0 0.0 0.0 0.0 46 38.30769230769231 0.0 0.0 0.0 0.0 0.0 47 38.92307692307692 0.0 0.0 0.0 0.0 0.0 48 38.61538461538461 0.0 0.0 0.0 0.0 0.0 49 39.38461538461539 0.0 0.0 0.0 0.0 0.0 50 38.92307692307692 0.0 0.0 0.0 0.0 0.0 51 38.92307692307692 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2116 1 0.0 2116 2 0.0 2116 3 0.0 2116 4 0.0 2116 5 0.0 2116 6 0.0 2116 7 0.0 2116 8 0.0 2116 9 0.0 2116 10 0.0 2116 11 0.0 2116 12 0.0 2116 13 0.0 2116 14 0.0 2116 15 0.0 2116 16 0.0 2116 17 0.0 2116 18 0.0 2116 19 0.0 2116 20 0.0 2116 21 0.0 2116 22 0.0 2116 23 0.0 2116 24 0.0 2116 25 0.0 2116 26 0.0 2116 27 0.0 2116 28 0.0 2116 29 0.0 2116 30 0.0 2116 31 0.0 2116 32 0.0 2116 33 0.0 2116 34 0.0 2116 35 0.0 2116 36 0.0 2116 37 0.0 2116 38 0.0 2116 39 0.0 2116 40 0.0 2116 41 0.0 2116 42 0.0 2116 43 0.0 2116 44 0.0 2116 45 0.0 2116 46 0.0 2116 47 0.0 2116 48 0.0 2116 49 0.0 2116 50 0.0 2116 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 35 2.0 36 0.0 37 3.0 38 1.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.076923076923077 38.46153846153847 15.384615384615385 23.076923076923077 2 30.76923076923077 15.384615384615385 46.15384615384615 7.6923076923076925 3 38.46153846153847 15.384615384615385 7.6923076923076925 38.46153846153847 4 7.6923076923076925 23.076923076923077 46.15384615384615 23.076923076923077 5 15.384615384615385 30.76923076923077 30.76923076923077 23.076923076923077 6 23.076923076923077 46.15384615384615 23.076923076923077 7.6923076923076925 7 7.6923076923076925 46.15384615384615 30.76923076923077 15.384615384615385 8 23.076923076923077 53.84615384615385 7.6923076923076925 15.384615384615385 9 7.6923076923076925 7.6923076923076925 38.46153846153847 46.15384615384615 10 0.0 38.46153846153847 38.46153846153847 23.076923076923077 11 46.15384615384615 15.384615384615385 38.46153846153847 0.0 12 0.0 30.76923076923077 46.15384615384615 23.076923076923077 13 30.76923076923077 23.076923076923077 30.76923076923077 15.384615384615385 14 15.384615384615385 7.6923076923076925 30.76923076923077 46.15384615384615 15 23.076923076923077 38.46153846153847 23.076923076923077 15.384615384615385 16 7.6923076923076925 53.84615384615385 15.384615384615385 23.076923076923077 17 23.076923076923077 53.84615384615385 15.384615384615385 7.6923076923076925 18 23.076923076923077 53.84615384615385 23.076923076923077 0.0 19 7.6923076923076925 30.76923076923077 23.076923076923077 38.46153846153847 20 46.15384615384615 30.76923076923077 15.384615384615385 7.6923076923076925 21 15.384615384615385 38.46153846153847 30.76923076923077 15.384615384615385 22 23.076923076923077 30.76923076923077 30.76923076923077 15.384615384615385 23 53.84615384615385 23.076923076923077 15.384615384615385 7.6923076923076925 24 7.6923076923076925 23.076923076923077 53.84615384615385 15.384615384615385 25 38.46153846153847 15.384615384615385 38.46153846153847 7.6923076923076925 26 23.076923076923077 38.46153846153847 23.076923076923077 15.384615384615385 27 23.076923076923077 23.076923076923077 30.76923076923077 23.076923076923077 28 15.384615384615385 38.46153846153847 23.076923076923077 23.076923076923077 29 15.384615384615385 38.46153846153847 7.6923076923076925 38.46153846153847 30 0.0 38.46153846153847 23.076923076923077 38.46153846153847 31 15.384615384615385 30.76923076923077 23.076923076923077 30.76923076923077 32 23.076923076923077 30.76923076923077 30.76923076923077 15.384615384615385 33 15.384615384615385 7.6923076923076925 53.84615384615385 23.076923076923077 34 30.76923076923077 7.6923076923076925 30.76923076923077 30.76923076923077 35 30.76923076923077 30.76923076923077 30.76923076923077 7.6923076923076925 36 7.6923076923076925 23.076923076923077 30.76923076923077 38.46153846153847 37 7.6923076923076925 38.46153846153847 30.76923076923077 23.076923076923077 38 38.46153846153847 30.76923076923077 15.384615384615385 15.384615384615385 39 15.384615384615385 46.15384615384615 30.76923076923077 7.6923076923076925 40 23.076923076923077 7.6923076923076925 30.76923076923077 38.46153846153847 41 23.076923076923077 38.46153846153847 15.384615384615385 23.076923076923077 42 0.0 23.076923076923077 38.46153846153847 38.46153846153847 43 38.46153846153847 15.384615384615385 30.76923076923077 15.384615384615385 44 7.6923076923076925 53.84615384615385 30.76923076923077 7.6923076923076925 45 15.384615384615385 23.076923076923077 30.76923076923077 30.76923076923077 46 15.384615384615385 15.384615384615385 46.15384615384615 23.076923076923077 47 23.076923076923077 30.76923076923077 23.076923076923077 23.076923076923077 48 23.076923076923077 15.384615384615385 23.076923076923077 38.46153846153847 49 30.76923076923077 7.6923076923076925 53.84615384615385 7.6923076923076925 50 0.0 38.46153846153847 38.46153846153847 23.076923076923077 51 15.384615384615385 23.076923076923077 30.76923076923077 30.76923076923077 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 2.0 33 4.0 34 2.0 35 0.0 36 0.0 37 0.0 38 1.0 39 2.0 40 2.0 41 2.0 42 1.0 43 0.0 44 1.0 45 2.0 46 1.5 47 1.0 48 0.5 49 0.0 50 0.5 51 1.0 52 0.5 53 0.0 54 0.5 55 1.0 56 0.5 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 13.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCTCATAATGCTCTCAGCGAATGTACGCACAGCACAGAAATGCATCCCTG 1 7.6923076923076925 No Hit GTCACAAAGATAGGAGAACACGACGGAACAGCTGTTCAAGGAGATGATGAT 1 7.6923076923076925 No Hit ATCATGGCTTGTGCATTAAGCCCTGTTACATTCTTCTCACAAAACTTCTTC 1 7.6923076923076925 No Hit ATGTTTACCTTTCACAATAGTGTTTCCTCCATCAATAGTCTCCACCGAGAC 1 7.6923076923076925 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATCTGTCTCTTAT 1 7.6923076923076925 No Hit TAAAAAAGCTACACAAGAAAAAGATGTAACTGGCGAAGTTTTGAGAACTCA 1 7.6923076923076925 No Hit AAGTAGAACAGAACAAGACTTAAACTGAACTATCAAAAACCCTGTCTCTTA 1 7.6923076923076925 No Hit ACCCTGCGTCGATTAACGTTGCGCAGGAACCCTTGGTCTTCTGTCTCTTAT 1 7.6923076923076925 No Hit GTAGTCTATCTTTTGTTAAGATGTGATCATATTGCCATAGAAATGTGGTTG 1 7.6923076923076925 No Hit TGGTGATGTAGTCCCCAAGGATGTGAACGCAGCGGTTGGCACCATCAAGAC 1 7.6923076923076925 No Hit CGGTAACATCTCTTTCGATGATGTGACTGAGATTGCTAGGATTATGAGGCC 1 7.6923076923076925 No Hit CGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATA 1 7.6923076923076925 No Hit GGGTAAAACAGTACTAATTATGGAATTGATCAACAACATTGCTAAAGCTCT 1 7.6923076923076925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE