FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_80.3410000000a477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_80.3410000000a477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAGAGTAATACGTCATCTAGTAGAAACCAGAGCGGGGAAGGATCTGT18.333333333333332No Hit
GTATATGATGGTGAAAACAGGGGAGAAGTAGAAGAGGATGATACATACAAC18.333333333333332No Hit
CTCCTTTACATGGCATCTCCACCACAAAGAAATATGCAATGAAACGTCCAC18.333333333333332No Hit
GACAATGGGTTAATATTCCCATACTTGCTCACACCTGTCTCTTATACACAT18.333333333333332No Hit
GTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCT18.333333333333332No Hit
AAGCAGATCTTTCAGAATGTGCAGATGAGCGTCTGTCTCTTATACACATCT18.333333333333332No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCCACCAGAGT18.333333333333332No Hit
GTGTTAAGGAAATTAACAAGCAAGGGAAAGAGGTTTTGTATGATTTTGAAG18.333333333333332No Hit
AATTATAAGGGTGTTGTTTGTATCTAACACACTTTGCACCTGTCTCTTATA18.333333333333332No Hit
GGTAGAAATGGGAACCTTAATCTCCATACGTGTACGAGCTGTCTCTTATAC18.333333333333332No Hit
CGTGTAGGAAAACCACGAATTGAAGTCCATCTTCTGTCTCTTATACACATC18.333333333333332No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)