##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_2_79.3410000000a46a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.357142857142854 0.0 0.0 0.0 0.0 0.0 2 35.07142857142857 0.0 0.0 0.0 0.0 0.0 3 36.0 0.0 0.0 0.0 0.0 0.0 4 35.857142857142854 0.0 0.0 0.0 0.0 0.0 5 36.785714285714285 0.0 0.0 0.0 0.0 0.0 6 39.42857142857143 0.0 0.0 0.0 0.0 0.0 7 38.57142857142857 0.0 0.0 0.0 0.0 0.0 8 39.285714285714285 0.0 0.0 0.0 0.0 0.0 9 36.357142857142854 0.0 0.0 0.0 0.0 0.0 10 35.857142857142854 0.0 0.0 0.0 0.0 0.0 11 38.714285714285715 0.0 0.0 0.0 0.0 0.0 12 39.0 0.0 0.0 0.0 0.0 0.0 13 38.5 0.0 0.0 0.0 0.0 0.0 14 39.0 0.0 0.0 0.0 0.0 0.0 15 38.714285714285715 0.0 0.0 0.0 0.0 0.0 16 39.0 0.0 0.0 0.0 0.0 0.0 17 39.57142857142857 0.0 0.0 0.0 0.0 0.0 18 39.42857142857143 0.0 0.0 0.0 0.0 0.0 19 38.857142857142854 0.0 0.0 0.0 0.0 0.0 20 38.57142857142857 0.0 0.0 0.0 0.0 0.0 21 38.714285714285715 0.0 0.0 0.0 0.0 0.0 22 39.142857142857146 0.0 0.0 0.0 0.0 0.0 23 37.642857142857146 0.0 0.0 0.0 0.0 0.0 24 39.42857142857143 0.0 0.0 0.0 0.0 0.0 25 39.0 0.0 0.0 0.0 0.0 0.0 26 38.285714285714285 0.0 0.0 0.0 0.0 0.0 27 38.285714285714285 0.0 0.0 0.0 0.0 0.0 28 38.285714285714285 0.0 0.0 0.0 0.0 0.0 29 37.214285714285715 0.0 0.0 0.0 0.0 0.0 30 37.0 0.0 0.0 0.0 0.0 0.0 31 38.57142857142857 0.0 0.0 0.0 0.0 0.0 32 38.42857142857143 0.0 0.0 0.0 0.0 0.0 33 38.857142857142854 0.0 0.0 0.0 0.0 0.0 34 37.92857142857143 0.0 0.0 0.0 0.0 0.0 35 38.285714285714285 0.0 0.0 0.0 0.0 0.0 36 36.642857142857146 0.0 0.0 0.0 0.0 0.0 37 38.42857142857143 0.0 0.0 0.0 0.0 0.0 38 37.357142857142854 0.0 0.0 0.0 0.0 0.0 39 38.714285714285715 0.0 0.0 0.0 0.0 0.0 40 38.357142857142854 0.0 0.0 0.0 0.0 0.0 41 38.857142857142854 0.0 0.0 0.0 0.0 0.0 42 38.714285714285715 0.0 0.0 0.0 0.0 0.0 43 39.142857142857146 0.0 0.0 0.0 0.0 0.0 44 38.57142857142857 0.0 0.0 0.0 0.0 0.0 45 39.285714285714285 0.0 0.0 0.0 0.0 0.0 46 37.214285714285715 0.0 0.0 0.0 0.0 0.0 47 38.142857142857146 0.0 0.0 0.0 0.0 0.0 48 38.57142857142857 0.0 0.0 0.0 0.0 0.0 49 39.142857142857146 0.0 0.0 0.0 0.0 0.0 50 36.5 0.0 0.0 0.0 0.0 0.0 51 38.42857142857143 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2108 1 0.0 2108 2 0.0 2108 3 0.0 2108 4 0.0 2108 5 0.0 2108 6 0.0 2108 7 0.0 2108 8 0.0 2108 9 0.0 2108 10 0.0 2108 11 0.0 2108 12 0.0 2108 13 0.0 2108 14 0.0 2108 15 0.0 2108 16 0.0 2108 17 0.0 2108 18 0.0 2108 19 0.0 2108 20 0.0 2108 21 0.0 2108 22 0.0 2108 23 0.0 2108 24 0.0 2108 25 0.0 2108 26 0.0 2108 27 0.0 2108 28 0.0 2108 29 0.0 2108 30 0.0 2108 31 0.0 2108 32 0.0 2108 33 0.0 2108 34 0.0 2108 35 0.0 2108 36 0.0 2108 37 0.0 2108 38 0.0 2108 39 0.0 2108 40 0.0 2108 41 0.0 2108 42 0.0 2108 43 0.0 2108 44 0.0 2108 45 0.0 2108 46 0.0 2108 47 0.0 2108 48 0.0 2108 49 0.0 2108 50 0.0 2108 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 36 3.0 37 2.0 38 5.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.857142857142854 21.428571428571427 7.142857142857142 28.57142857142857 2 0.0 14.285714285714285 57.14285714285714 28.57142857142857 3 7.142857142857142 21.428571428571427 14.285714285714285 57.14285714285714 4 21.428571428571427 28.57142857142857 21.428571428571427 28.57142857142857 5 14.285714285714285 42.857142857142854 42.857142857142854 0.0 6 42.857142857142854 42.857142857142854 7.142857142857142 7.142857142857142 7 7.142857142857142 28.57142857142857 42.857142857142854 21.428571428571427 8 14.285714285714285 50.0 21.428571428571427 14.285714285714285 9 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 10 14.285714285714285 28.57142857142857 57.14285714285714 0.0 11 21.428571428571427 35.714285714285715 21.428571428571427 21.428571428571427 12 21.428571428571427 28.57142857142857 21.428571428571427 28.57142857142857 13 14.285714285714285 7.142857142857142 50.0 28.57142857142857 14 21.428571428571427 21.428571428571427 28.57142857142857 28.57142857142857 15 21.428571428571427 21.428571428571427 28.57142857142857 28.57142857142857 16 14.285714285714285 21.428571428571427 35.714285714285715 28.57142857142857 17 21.428571428571427 28.57142857142857 35.714285714285715 14.285714285714285 18 21.428571428571427 21.428571428571427 28.57142857142857 28.57142857142857 19 21.428571428571427 28.57142857142857 28.57142857142857 21.428571428571427 20 21.428571428571427 57.14285714285714 14.285714285714285 7.142857142857142 21 14.285714285714285 42.857142857142854 35.714285714285715 7.142857142857142 22 7.142857142857142 14.285714285714285 64.28571428571429 14.285714285714285 23 14.285714285714285 28.57142857142857 50.0 7.142857142857142 24 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 25 14.285714285714285 50.0 14.285714285714285 21.428571428571427 26 35.714285714285715 28.57142857142857 14.285714285714285 21.428571428571427 27 35.714285714285715 21.428571428571427 21.428571428571427 21.428571428571427 28 28.57142857142857 21.428571428571427 28.57142857142857 21.428571428571427 29 14.285714285714285 50.0 21.428571428571427 14.285714285714285 30 42.857142857142854 21.428571428571427 28.57142857142857 7.142857142857142 31 21.428571428571427 28.57142857142857 28.57142857142857 21.428571428571427 32 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 33 21.428571428571427 21.428571428571427 42.857142857142854 14.285714285714285 34 14.285714285714285 35.714285714285715 28.57142857142857 21.428571428571427 35 42.857142857142854 35.714285714285715 14.285714285714285 7.142857142857142 36 21.428571428571427 28.57142857142857 21.428571428571427 28.57142857142857 37 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 38 14.285714285714285 14.285714285714285 35.714285714285715 35.714285714285715 39 21.428571428571427 21.428571428571427 42.857142857142854 14.285714285714285 40 28.57142857142857 21.428571428571427 21.428571428571427 28.57142857142857 41 21.428571428571427 28.57142857142857 28.57142857142857 21.428571428571427 42 0.0 35.714285714285715 57.14285714285714 7.142857142857142 43 21.428571428571427 28.57142857142857 28.57142857142857 21.428571428571427 44 28.57142857142857 14.285714285714285 28.57142857142857 28.57142857142857 45 14.285714285714285 14.285714285714285 35.714285714285715 35.714285714285715 46 14.285714285714285 14.285714285714285 35.714285714285715 35.714285714285715 47 7.142857142857142 28.57142857142857 35.714285714285715 28.57142857142857 48 7.142857142857142 21.428571428571427 42.857142857142854 28.57142857142857 49 28.57142857142857 42.857142857142854 21.428571428571427 7.142857142857142 50 7.142857142857142 35.714285714285715 35.714285714285715 21.428571428571427 51 21.428571428571427 28.57142857142857 28.57142857142857 21.428571428571427 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.5 33 1.0 34 0.5 35 0.0 36 1.5 37 3.0 38 2.0 39 1.0 40 2.5 41 4.0 42 3.5 43 3.0 44 1.5 45 0.0 46 1.0 47 2.0 48 1.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 14.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCAGAAATCACATTGCGTTAGTATCCGCAGGGACCATCGCAATGCTTTGTT 1 7.142857142857142 No Hit CACCTTCTCTATCGCCTCCTTCTTCAATCTCATCGCAATCATGATCATATC 1 7.142857142857142 No Hit ATCAAGTAGTCATCCTTCACAATACCGTAGTGTGGGACTGTCTCTTATACA 1 7.142857142857142 No Hit GTCCAGTTTTACCAATGACAATGGAAGCAATGTTGGCTAATACCTGAAGTG 1 7.142857142857142 No Hit GTCTTGTAAGAGGCTAAGGATCATGAAAGGATCTGAAGTTAAAGGTTTAGG 1 7.142857142857142 No Hit TCTTTGAATTTCTACTTGTATTTAAGCTTCTTGATTCACCTTAAACCCGAG 1 7.142857142857142 No Hit CTTCAGCAATGATCTCTTTGATGGATTTATCATCACCCATGTTGCTGTCTC 1 7.142857142857142 No Hit ACCTAAACGAGCCAAACCGAAATCTGAGAGCTTTGCTTTCCAGTCCTCATC 1 7.142857142857142 No Hit ATCAGACATATCAGAAGTGACTAGATCATGAGAGAAGTGACTAGATCATCA 1 7.142857142857142 No Hit GTAGTGTGATATGTGGACTTGTTGAGTGGTGTTAAATCTTGTTTCCCTTAT 1 7.142857142857142 No Hit CTACTAGCTGAGTTTGAGAATGCTAGGGTATTGATCACTGATCCTGTCTCT 1 7.142857142857142 No Hit GAGGGAAAAAGATGCTCAAGTTAATCTGCAACCAAGTTGGTTCCTCCAAAA 1 7.142857142857142 No Hit CCCAACTACTCTCTGTTTCAATTCGAGAAGATGAATCGCGCAGTCATGAAT 1 7.142857142857142 No Hit GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAA 1 7.142857142857142 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 7.142857142857143 39 0.0 0.0 7.142857142857143 >>END_MODULE >>Kmer Content pass >>END_MODULE