##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_2_78.3410000000a45e.fastq.gz File type Conventional base calls Encoding Illumina 1.3 Total Sequences 8 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 7.0 0.0 0.0 0.0 0.0 0.0 2 7.0 0.0 0.0 0.0 0.0 0.0 3 6.25 0.0 0.0 0.0 0.0 0.0 4 6.25 0.0 0.0 0.0 0.0 0.0 5 6.25 0.0 0.0 0.0 0.0 0.0 6 8.5 0.0 0.0 0.0 0.0 0.0 7 8.5 0.0 0.0 0.0 0.0 0.0 8 8.75 0.0 0.0 0.0 0.0 0.0 9 8.25 0.0 0.0 0.0 0.0 0.0 10 8.5 0.0 0.0 0.0 0.0 0.0 11 8.75 0.0 0.0 0.0 0.0 0.0 12 8.5 0.0 0.0 0.0 0.0 0.0 13 8.75 0.0 0.0 0.0 0.0 0.0 14 8.25 0.0 0.0 0.0 0.0 0.0 15 7.75 0.0 0.0 0.0 0.0 0.0 16 8.25 0.0 0.0 0.0 0.0 0.0 17 8.25 0.0 0.0 0.0 0.0 0.0 18 8.75 0.0 0.0 0.0 0.0 0.0 19 8.25 0.0 0.0 0.0 0.0 0.0 20 8.0 0.0 0.0 0.0 0.0 0.0 21 8.5 0.0 0.0 0.0 0.0 0.0 22 8.5 0.0 0.0 0.0 0.0 0.0 23 8.75 0.0 0.0 0.0 0.0 0.0 24 8.0 0.0 0.0 0.0 0.0 0.0 25 7.5 0.0 0.0 0.0 0.0 0.0 26 8.5 0.0 0.0 0.0 0.0 0.0 27 8.5 0.0 0.0 0.0 0.0 0.0 28 8.5 0.0 0.0 0.0 0.0 0.0 29 8.25 0.0 0.0 0.0 0.0 0.0 30 8.5 0.0 0.0 0.0 0.0 0.0 31 8.5 0.0 0.0 0.0 0.0 0.0 32 8.75 0.0 0.0 0.0 0.0 0.0 33 8.0 0.0 0.0 0.0 0.0 0.0 34 9.0 0.0 0.0 0.0 0.0 0.0 35 8.75 0.0 0.0 0.0 0.0 0.0 36 8.75 0.0 0.0 0.0 0.0 0.0 37 9.0 0.0 0.0 0.0 0.0 0.0 38 8.0 0.0 0.0 0.0 0.0 0.0 39 8.5 0.0 0.0 0.0 0.0 0.0 40 9.0 0.0 0.0 0.0 0.0 0.0 41 8.25 0.0 0.0 0.0 0.0 0.0 42 8.25 0.0 0.0 0.0 0.0 0.0 43 8.5 0.0 0.0 0.0 0.0 0.0 44 8.0 0.0 0.0 0.0 0.0 0.0 45 8.5 0.0 0.0 0.0 0.0 0.0 46 8.5 0.0 0.0 0.0 0.0 0.0 47 8.0 0.0 0.0 0.0 0.0 0.0 48 8.25 0.0 0.0 0.0 0.0 0.0 49 8.5 0.0 0.0 0.0 0.0 0.0 50 9.0 0.0 0.0 0.0 0.0 0.0 51 8.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores fail #Quality Count 7 3.0 8 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.5 25.0 12.5 25.0 2 0.0 37.5 50.0 12.5 3 50.0 12.5 25.0 12.5 4 0.0 12.5 37.5 50.0 5 25.0 25.0 37.5 12.5 6 12.5 25.0 37.5 25.0 7 0.0 25.0 25.0 50.0 8 12.5 62.5 25.0 0.0 9 37.5 12.5 12.5 37.5 10 0.0 50.0 25.0 25.0 11 75.0 12.5 12.5 0.0 12 25.0 12.5 25.0 37.5 13 12.5 37.5 37.5 12.5 14 0.0 50.0 37.5 12.5 15 50.0 12.5 25.0 12.5 16 0.0 37.5 25.0 37.5 17 12.5 25.0 62.5 0.0 18 12.5 12.5 50.0 25.0 19 12.5 37.5 25.0 25.0 20 0.0 25.0 50.0 25.0 21 12.5 37.5 25.0 25.0 22 37.5 37.5 12.5 12.5 23 25.0 12.5 50.0 12.5 24 37.5 37.5 12.5 12.5 25 12.5 12.5 50.0 25.0 26 25.0 25.0 25.0 25.0 27 25.0 25.0 37.5 12.5 28 12.5 37.5 50.0 0.0 29 25.0 50.0 25.0 0.0 30 37.5 37.5 12.5 12.5 31 25.0 0.0 37.5 37.5 32 25.0 25.0 25.0 25.0 33 0.0 62.5 37.5 0.0 34 0.0 62.5 37.5 0.0 35 50.0 12.5 12.5 25.0 36 0.0 37.5 12.5 50.0 37 37.5 37.5 12.5 12.5 38 25.0 25.0 50.0 0.0 39 12.5 62.5 12.5 12.5 40 25.0 25.0 25.0 25.0 41 12.5 37.5 25.0 25.0 42 0.0 37.5 37.5 25.0 43 50.0 25.0 12.5 12.5 44 25.0 50.0 12.5 12.5 45 25.0 25.0 25.0 25.0 46 12.5 25.0 37.5 25.0 47 25.0 0.0 50.0 25.0 48 0.0 0.0 50.0 50.0 49 12.5 25.0 50.0 12.5 50 37.5 37.5 25.0 0.0 51 12.5 12.5 75.0 0.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 1.0 28 0.5 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.5 35 1.0 36 1.0 37 1.0 38 1.5 39 2.0 40 1.0 41 0.0 42 0.5 43 1.0 44 0.5 45 0.0 46 1.0 47 2.0 48 1.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 8.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTGTATGCGTATGATAGCGACGGTTGATGGAAACGGTGACGGTTTTGTT 1 12.5 No Hit CTTCACCACAGGGAGCTTTTCGGGTAGTGATCTTGAGAACCTGTCTCTTAT 1 12.5 No Hit CTGTTCAAGAGCATTCGGATTATACATAAACAAACCGTAGAAAAGCTCAAA 1 12.5 No Hit GAGTGTTATTGCTACTTCTTTGTATGATGATTATGACTAAAAGCTTTTATT 1 12.5 No Hit GCTATTCTCCATCCTCATATCTTGTTGAAGGCAACAATGTCACAACTCTGG 1 12.5 No Hit ATCCTACACAGATTGATTCAAAGTCCAATGCAAAGCTACAGTAAAGGCTGT 1 12.5 No Hit AAACCGTAAAGCAAGATCACACAAAGCTTGTGTTGTAGATTCTACAGTCAT 1 12.5 No Hit TTGCAACGGTTGTAATATCAAGTCTCTTACCTTATCATACTTGGGACCTGT 1 12.5 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE