FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_75.3410000000a427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_75.3410000000a427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCATTACCATTGGTCCATTGTCTAGCTTAAATATATCCCTGTCTCTTA13.225806451612903No Hit
ACTACTACCACCACATTTCTTTAGCTCAACCTTCATTACTAATCCTGTCTC13.225806451612903No Hit
GACAATTGACAGGGAACCTTGGAGAGATCTTGATCAGGTGCAACAATGTTT13.225806451612903No Hit
GTATAGACTTGTAAGATGTTGTACTGTCTTGTGCTTTACTGAGATAGATAG13.225806451612903No Hit
CTCAGGGACACTATCATCAGCATGAACAAAGTATTGCTTAACCACTGAGTA13.225806451612903No Hit
GTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTATATAGGGACTGTCTCTT13.225806451612903No Hit
ATGTTATCTTGAAGTGCTACTTGATCGGAAGAAGGTTTAGCCACTGTCTCT13.225806451612903No Hit
ACATTGAACTGATACACTTCCTGCTGACTAAGATCAGATTCAGAAGCTTTG13.225806451612903No Hit
CAATTTGGCCTTTATGAGATGTCTCCAACAGATTGAATAGCTTTGATAATG13.225806451612903No Hit
GCTAAAGAAAGATACGAAGCAGCTAAAAAAGCTGAAAAAGCAGCAGCTAAA13.225806451612903No Hit
CATTAGTAGTAGTCTAGGAAGACTAGTAGTAGTAGCAACGGATGTTATTTG13.225806451612903No Hit
GCCACCACCGAAGGGACTGGTGAGGGCAAAGTCAATGTGCTTCTGTAAATC13.225806451612903No Hit
GCCATATCTCCCCTAGGATGGTTTTGACTTTCGAATGTTATCTCTGTCTCT13.225806451612903No Hit
ACACGAAGATAACAATTTCGCTTCAAGGAAATAAATCTGAAGACAATCTTA13.225806451612903No Hit
GAGAGAGAGAGAGAGAAAGAGAGAGCGAGAGAGATAGAGCTGTCTCTTATA13.225806451612903No Hit
GTTACTAGCGATAGTAACGAAGTGATTGATCCTACACTGTCGAGAAAAGCT13.225806451612903No Hit
TGTGATGAGTTTGCGTGTGAAATTGACAAAAATTTGAGTTGTGATGGTACT13.225806451612903No Hit
ATGCCATGTAATCAACTGATTGTTACACAAGCATTTTCTAAAGCTATGACA13.225806451612903No Hit
CAAAAGCATATTCAATAGTAAATTCAAGATCATACGATTCTCTTAAACAAG13.225806451612903No Hit
GAACCAACCATTGTTCAAAATAGTACTAACCAAAAAACACCACAAAATGGG13.225806451612903No Hit
CAGTTGACCTGCGCGATATACCAAAATTAAGAGATGTTATATCCCTGTCTC13.225806451612903No Hit
TTCATGAACTGGTCAATCAGATAGCCATTAGCCTGTCTCTTATACACATCT13.225806451612903No Hit
CCCTAACGGGGAAGACCATAACCCTTGAGGTTGAATCATCCGACACCATCG13.225806451612903No Hit
ACTTGTTACTATGTGAAGATGAAGAGAAAATGGAGTTTGAATCGAAGAAGT13.225806451612903No Hit
TCGTAGTGCCTTCTCTTTTATTGTTCGATCTTCAATCTCTGTCTCTTATAC13.225806451612903No Hit
TTCCATCATTATCCAACTTTTTAAACGACAATCTGAAAGAACGAGATCCAT13.225806451612903No Hit
ATCAACGCAGAGTACGGGACTGCACATGAAAACTAAACACACGTACACAAG13.225806451612903No Hit
TTTCTATTCTCTTACTTAGCAGATAGTGCATCAATAGTAGCTGTCTCTTAT13.225806451612903No Hit
CTTGTGATATCTGCAGATAACTTCGTATAATGTATGCCTGTCTCTTATACA13.225806451612903No Hit
GAATTGGTTGTGTTTCTGTGAATTCTTTTTCTACTTCTGTCACCACTGGTT13.225806451612903No Hit
ACACAATGAATGGCGCGTGAAAAGATCTAATTATATGAAAAGATGGACAGC13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)