FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_74.3410000000a41a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_74.3410000000a41a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACTCCTCTTCCAGGAGTTTATGCAACTTTGCTTCAGTTCGCCGATA12.857142857142857No Hit
CTGTAGCTTATTAGCTGATTCATTCTCCATAGTCCCACTGAGCTCTCGCTG12.857142857142857No Hit
ACATAATCCATGTAGCTATGTATAAAGATACGATGCTTAATCTGTCTCTTA12.857142857142857No Hit
GTTCGCAAACGCGTTGGCTGAGAGCAAAAGTGCAGCAGCGGCCAGCAGAGT12.857142857142857No Hit
AACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGG12.857142857142857No Hit
ATGTTTACAGTATGCTGTGTGTGATTTTTTATGCCTTTTTCTTCTTTGTGA12.857142857142857No Hit
GTGCCAAGAAAAGCTTCTAAACGTTGAAACATGATTGCCCCTGTCTCTTAT12.857142857142857No Hit
TATCTGGACAAGGTTAATATTTCTTTGTGGCCTCGTTTCAAGATGGTGTTT12.857142857142857No Hit
CATTAGTAAATGGATTCAGGTTTTAGTGACTAGTAGAGTGATCACGTGTAA12.857142857142857No Hit
ACATTAGGGTGTCTAGAAAAAAAAATCAGCACTTCCTGATCATAAATCAAG12.857142857142857No Hit
TTCATACAAATTGGATAAAAATTACAACGAAACCGGACCAAACTCCAAACA12.857142857142857No Hit
CTACCAGACCACGTTCAGTGAGCTCTACCCCTGAGTTTTCGCCAAGTAGGT12.857142857142857No Hit
GGCCAAAGACTCTTACCAAGCCTAGAACTGGTAAGTACCCAAACTGTCTCT12.857142857142857No Hit
GACTACAACATCCAGAAAGAATCTACACTTCATCTTGTGTTGAGGCTTAGA12.857142857142857No Hit
CTTAGGGGCCTTAGCCGGCGATCTGGGCTGTTTCCCTCTCGACTACGAAGC12.857142857142857No Hit
GACTTGTTTAACAACACGAAATTTAATGAAGAGGGAGATAATTCCCTATAA12.857142857142857No Hit
TTACGCTTTTCCATTACACAAGAAAGAAAAAAAGGTACACGAATAATGCAA12.857142857142857No Hit
TAGTAGTGCTGAGGATATGCTTAAAGCTCTCTGATTCTTATGATTTTCTCA12.857142857142857No Hit
GTTGTTGCAAATGCTCGCAGCATTTGCCGCCGCCGCAGCCGCAAGACATTC12.857142857142857No Hit
ATAACAAGCATCATACCACCAGAAGCAGAGGTCGTGTCTTCAAGTTGATGA12.857142857142857No Hit
CTACTTATATTAGCTAATGTTTTTGACTTGGGATTTTGTCATTGGACCATT12.857142857142857No Hit
ACACAGCACCACAGCCCAAACTCCTCAAATGCTCCAAAATCTTGAGATTGT12.857142857142857No Hit
GTAAAGGACTTGGTAGAAACTCGGGTAGGTGAGGCAGATCATACCCATCTT12.857142857142857No Hit
TTTCAATTCCTTTATCCACTCATTCATGATTCCTCCTCCTGTCTCTTATAC12.857142857142857No Hit
GATGAGGATGTTATTAGAATCTTGTCCACAAGAAGCAAAGCTCAGATCAAT12.857142857142857No Hit
AAATAAATGGTTCTTTGTCAAATTTGGTTAACAAAAAAAAAAAAAAAAAAA12.857142857142857No Hit
GATATTACACTTATTTTAAGAAGACTGGAATCGCTAAACGTGTTATGATGT12.857142857142857No Hit
ATCAAACGGAATGGGACGAAGCTTATCAACTTCCATGTAGAACAAGGAAGC12.857142857142857No Hit
CAAGATGAAGGGTGGACTCCTTCTGGATATTGTAGTCAGCAAGAGTTCTGC12.857142857142857No Hit
GATGTAACTTTTTATCATAATTCGAGAAGAGTAACTTCGCTGCCAAAAAAA12.857142857142857No Hit
GTACGGGACAACAACTAAAACCACATTTATCATTACCTGTCTCTTATACAC12.857142857142857No Hit
ACTGGATCTGCAACCCTGTGCACAAGCACAGAGAACTCAGAGGTCTCACCT12.857142857142857No Hit
GACTACAACTCAAAGCTTCTTGAATCAAGTAATTATATAACCAGACGGCTG12.857142857142857No Hit
GGCATATTGTTTTCAGTGTCAAAAACCCTCGAAAATGCCAGAGTTCAGAAA12.857142857142857No Hit
TCTCATATCCATCAAAGAAGATGTATTGACATCCTCCACAAATCCAAGGCT12.857142857142857No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)