FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_71.3410000000a3d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_71.3410000000a3d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC22518.174474959612276No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT100.8077544426494345No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC40.32310177705977383No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGACGTC30.24232633279483037No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATTCGTATGCCGTCT30.24232633279483037No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGTTATATCTCGTATGCCGTC20.16155088852988692No Hit
CTTATACACATCTCCGAGCCCACGAACGACGCTATATCTCGTATGCCGTCT20.16155088852988692RNA PCR Primer, Index 11 (95% over 21bp)
CTTATACACATATCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC20.16155088852988692No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG20.16155088852988692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATC252.0214775E-545.033
ATCTCGT252.0214775E-545.037
TATATCT252.0214775E-545.034
TCTCGTA252.0214775E-545.038
TATCTCG252.0214775E-545.036
ATATCTC252.0214775E-545.035
CTCGTAT252.0214775E-545.039
AGCCCAC308.5263673E-744.99999617
ATCTCCG308.5263673E-744.99999610
CACATCT308.5263673E-744.9999967
GCCCACG308.5263673E-744.99999618
CCACGAG308.5263673E-744.99999620
CATCTCC308.5263673E-744.9999969
GAGCCCA308.5263673E-744.99999616
CACGAGA308.5263673E-744.99999621
CCCACGA308.5263673E-744.99999619
CGCTATA308.5263673E-744.99999631
ACATCTC308.5263673E-744.9999968
ACACATC308.5263673E-744.9999966
GCTATAT308.5263673E-744.99999632
Produced by FastQC (version 0.11.2)