FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_7.34100000009f8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_7.34100000009f8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC42.547770700636943No Hit
ATCTTAATGAGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC10.6369426751592357No Hit
GAAGAGGGGCTTGCTGGTTTTTCCATCATCCAATCCACAATAAATCGAATG10.6369426751592357No Hit
CTGCAGAGGATGGTTCTGTTTTTGAGGTTCCTTGTGATTCAGGTTTTCAGC10.6369426751592357No Hit
GGTGGAGACCTTCGACGAGGAGCTTCCCCGCCTGTTCTGTCTCTTATACAC10.6369426751592357No Hit
ACATTGTAGTTACCTCACCACCAACACAGTAATTAAGCCTCCAAGAAGAGT10.6369426751592357No Hit
CTTCTTCTCCTCTGATCTCATACCAAGTGCTGCTTGTCTCTTCTTCCTGTC10.6369426751592357No Hit
GGTAGGAAGAGAGTGAACAAGACTATGAATCCAAATCTTGGGCTTTGGAGT10.6369426751592357No Hit
GTCCAGTTTTCATCAGAAGGAGGTTCATAGGTTTCAGGTAGAACTTGCATA10.6369426751592357No Hit
CTTTGAAAAAGCTTAACAAGAACAAGAAGCTTGTTAAGAAGCTTGCTACTG10.6369426751592357No Hit
ATAGAGGAATCTCAAAAGGCAGAGAAGCACTAAAAGAAATGGTCAACATCC10.6369426751592357No Hit
TGACTTGGGTATAGGGGCGAAAGACTAATCGAACAATCTAGTAGCTGGTTC10.6369426751592357No Hit
CTCTATTGCACTCCCGTCATAGGGTTCTTTTCACCTTTCCCTCACGGTACT10.6369426751592357No Hit
GTCTCACAGTCAGGCAGGCTTATACCATTACGCTCACGAGCAGAATCTTAG10.6369426751592357No Hit
GTAGTATAGACCATTCCAGAACAGAGTTTCTTGGGTCTGTCTCTTATACAC10.6369426751592357No Hit
GGATATATGGTTGGTTCTTTGTGCCTGCTTTCTTCTACTTATCATAGCTGT10.6369426751592357No Hit
AGAGAGCAGAGCGTACCGCCCTGCCATAGTCGCGAGTCTGTTTATAGTCGC10.6369426751592357No Hit
AGATACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGCGCTGTGCGT10.6369426751592357No Hit
TAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC10.6369426751592357No Hit
CAAGCAGTCATGGCACCATATGCGTGTTATGGCGTGCCTATTGAAGCATGA10.6369426751592357No Hit
TATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCCTGTCTCTTATA10.6369426751592357No Hit
GTACAAGAGAGGCACTTTCTGACCTATCTGTGTACTGTGAAACTGTTTCTC10.6369426751592357No Hit
CTACTGAAGGTCGCCAAACTACGACGAGACTTTCGCCTTTTGAAGCGCTAG10.6369426751592357No Hit
GGCAGGGGCAATTACCATGTTATTAACCGATCGTAACTTTAATACAACCTT10.6369426751592357No Hit
TCTCAGGCTTGATCTCAACCTGGTTGAAAGTCTTTCCGTTGTACACTCCAA10.6369426751592357No Hit
CTTCAGAAGAAGGAAATTCTTGGTAGTAACTTAAACCTGGAAGATTCATTC10.6369426751592357No Hit
GTGTGTATTTCCCATTTTCTGTGATTTTCAGTTTTCTCGCCATATTCCAGG10.6369426751592357No Hit
TTTCCCTTCCTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTCGAAGC10.6369426751592357No Hit
GCACACATCTTCTCTTCTACCTTTACGTGATTCATATTCACACCTGTCTCT10.6369426751592357No Hit
ATATGAAGTTATGGAAGCCCTGAGTTTTCTCATCATCAAGTCTTGTTTTCT10.6369426751592357No Hit
TCCTTTACCCAATCACCAAAAAGAGTGAAGAAGAGAGAATCTGTCTCTTAT10.6369426751592357No Hit
GTTCCGGGACGTGCTTCCACGGTGTCCCGAGCATTGCCCGGGTGGTCTGCA10.6369426751592357No Hit
CCTTATAGACCAAGATGGCCGTGTCTTTGCCCGGACCGCCCTGGGTGGTGG10.6369426751592357No Hit
CCATGAACGCTGAGAAGTCAAATTCCCATATCAAAGCAATAAGCATGAAAC10.6369426751592357No Hit
GAGTTAAACCAAAAGATAGATGCTGATTTTCTTCAGTACTTGATCTGTCTC10.6369426751592357No Hit
CCACTATACGACTTCAAAAATGACGAAATCACTGAAAAACGTGAAAAAAGG10.6369426751592357No Hit
CTTCGGCGAAGAAGGATATACGCAATTATCTATGCCAGTATTGCGGAATCA10.6369426751592357No Hit
CATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAA10.6369426751592357No Hit
GATCAGAACTGGAGACGCAGAAAGATGCATTTGATGTCTCGGTCTGTCTCT10.6369426751592357No Hit
TAACAGTAGTCTCCTCAGGAACAACAGCAGGAGCAATAACTGTCTCTTATA10.6369426751592357No Hit
TTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAAC10.6369426751592357No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGG10.6369426751592357No Hit
GTCCCCGTTCGCTCGCCACTACTAAGGGAATCACTTTTGTTTTCTTCTCCT10.6369426751592357No Hit
TATTCGGGACTCACATTAAAGCTGACTATGGCGTGAGCCGCATACTTTTCT10.6369426751592357No Hit
GGAAGGGAATGGGGAATGTTTTTTTACACAGCATTCTCGCTGTCTCTTATA10.6369426751592357No Hit
GAGTACGGGCCGTGTGAAAGCTCGCTAGAGGCTTTTCTCGGCAGCATAGGA10.6369426751592357No Hit
ATATACTTGTTGCAAGCAATACGTGCTGCTTCAAGTGCATCACTTGACACA10.6369426751592357No Hit
GCTGTAAGAAGAACCTTCAGTACTTGGAGTACAGTGCTGTCTGGTGATGAA10.6369426751592357No Hit
GAAGGTGATGATGATGATGCTTCTCCTGTAATATGTCCTGTGAAACCACCC10.6369426751592357No Hit
CTTCAGAAGGTCTCCTGTATCCTCCAGAAAATCCATTCTCATTTCCAGGAG10.6369426751592357No Hit
CTTCATAGGAGTCAAGATGATCCTTGATTTCTTTGGCCTGTCTCTTATACA10.6369426751592357No Hit
CTGTAAGAGGTTGTGGAGGCGCGAACCCACGTAGGTTAAAAACTGCGGGGA10.6369426751592357No Hit
GCACTATCTGTCGCTTGCCGGGCCTCGAGATCCGCTAGAAAGTCGTGCTCG10.6369426751592357No Hit
CGGTTAATGGAGCTCCTAGTGTCGGAATCTCTGTTAAACATGTCTCTTATA10.6369426751592357No Hit
GGTTCGTAGGTAGAGTATACTAAGGCGTAGAGATAAGGCTAGTGAAGGAAC10.6369426751592357No Hit
GATAAATGGGACCTCATGAAACTGGAAAGCTTCTGTAAGGCAAAGGACATA10.6369426751592357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.6369426751592357No Hit
CGCCGCGACCGCGATTCATGAACAAAAAGTTCTGCGCGGTGATCTGCCGCA10.6369426751592357No Hit
TATCAACGCAGAGTACGGGAAGAGGCGCTACGCTGCCCTCGGCCCGATCAT10.6369426751592357No Hit
TTGTGGTGCCTCTCTCCAGAAAATGGTGACAAAAATTGAAATCAACCAGCA10.6369426751592357No Hit
ACGGAAAGTGGCGGGGCCGACACGGGCTCACCGGAGGTACAAGCGGCTCTT10.6369426751592357No Hit
CTATGAGTTGGATTGTTTAGAATCTGGTTGATCTAGAACTTAGCTGGTTCA10.6369426751592357No Hit
ACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTA10.6369426751592357No Hit
GAATAAGATTCCCATCTTCTTCTTCGATTGGATCAGATCAAACGATCTAAG10.6369426751592357No Hit
TTTCATCTCAGCTTCCGTAGCTTTCCTCTTCTTCTCCCTCTTATACCTGTC10.6369426751592357No Hit
ATCTTCATGAGCTTAGAAGAAGAACCATCTCGCTTAGTATCGCTTCGAGTC10.6369426751592357No Hit
GCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTC10.6369426751592357No Hit
GGCCGTGTACATGCCCTTGGGCGACCACCGGGATGGATACCGTGCGACCAC10.6369426751592357No Hit
CTGTAGCCGTAAATGGCCCACAGCACCGTGATGAGCGAAAACACCATCATC10.6369426751592357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTCTGTGAGACTCCCCCCGTCCCTCT10.6369426751592357No Hit
GTTCCTTAGGGACCGATTCACTCTGCGTAGATTGACTGAACGCAGAAAGCC10.6369426751592357No Hit
CTTATTGATATGCTTAAACTCAGCGGGTAATCCCGCCTGACCTGGGGTCGC10.6369426751592357No Hit
CACTAACAAAGTTGTTTTAGGTCTCTTAACCAAAGAGATCAAATCCAAAGT10.6369426751592357No Hit
ATTAGAAACAGGGGAAATCTCCTTAGGTACAGCTCTTTTGACCTCAACTAG10.6369426751592357No Hit
AAGCCATAGTGAGCTAAGATGATGTCTTCTTGTTCTTTCGTTATATTTTGA10.6369426751592357No Hit
GTAAAACGCGGCGTGTTCGAATTCTGATCGCTTTTACGCGAGAAAGGGGGA10.6369426751592357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACCCCC10.6369426751592357No Hit
CGTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCGC10.6369426751592357No Hit
GTATAAGGTAGGAGCTAAACAGAACATAAAGCATAAGAGGTTGATTCCATC10.6369426751592357No Hit
CTCCTCTGGTTTTGTTTCGTCTTTCTTCTTTCCCAAGACTGTCTCTTATAC10.6369426751592357No Hit
GTTATCCCCGGCGTACCTTTGATCCGTTGAGCGAGAGCCCTTCCACACGGG10.6369426751592357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTT10.6369426751592357No Hit
CCTACACCTAATGGCTTAAGCCGCTCTTCCCATTTCCTCGCTGACCCATCA10.6369426751592357No Hit
CGGCTCAACAAAGCAGCCGACACGTCCTACCTATTTCTGGCTCTTATACAC10.6369426751592357No Hit
CCTTTGAAGAAAGCGTAATAGCTCACTGGTCTAGCTCCATGGCACCGAAAA10.6369426751592357No Hit
TGAAGTTGGTGCTTGATTAACGGTACTTAATGAATCCAATCTCCTTAGCGC10.6369426751592357No Hit
CGTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCA10.6369426751592357No Hit
GCACAGATCTTTAGGGTTTTAGATTGTCTCATAACAAGACGAACTTTTCCA10.6369426751592357No Hit
GGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCCTGT10.6369426751592357No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT10.6369426751592357No Hit
GTAAGGATTTCGTGTTGTGGTAACCTTTTTGACTTAAGTTGGTTAAGAATC10.6369426751592357No Hit
TTCCAAAATGCAAATTCGGGATTTCTGCAAAGCTATGGACTTTCCAATGAA10.6369426751592357No Hit
GGTTTGTTCCCATTAGCGTCAACACAATTAGGTGAAGCAGAAGCATCAAGC10.6369426751592357No Hit
CTCTTCTCTTTTGAAGGCTTTTGCATTAGGGCAACAAGAATGGTTCATACA10.6369426751592357No Hit
GGACAAATAGGAAAATTGTCGATTATTTTGGCCTCTATGCAGACTGTCTCT10.6369426751592357No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG10.6369426751592357No Hit
ATCTTCTAGTTCTTCCTATTTGACCCGAAACCGATCGATCTAGCCATGAGC10.6369426751592357No Hit
TCTTAGGATCAACTTGAAGACCTTCCTTGATCTGTCTCTTATACACATCTC10.6369426751592357No Hit
GGTTTTTGGGGCTTAACCCATATTTAACCTACTTTTCATTTTGCTGCACCT10.6369426751592357No Hit
GTTGGAGTTGCTCCTAAAGGAACTGTTGATGTTCATGAGATGTTGTTTAAG10.6369426751592357No Hit
GTTACATAGAAAGATGCTGTCTTAAAACCAACAAATTCAAGCCCAGTCCCT10.6369426751592357No Hit
AGTATACCGAGGCGATCGAGTGAATCGTGGTGAAGGAACTCGGCAAAATGC10.6369426751592357No Hit
TCTGAGGAACAGTATCGAAATCGTCTCCAAAGTCGTACCTCTGTCTCTTAT10.6369426751592357No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCA10.6369426751592357No Hit
TATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATTCCGAGCC10.6369426751592357No Hit
ACTTCATTCTTTTCGTTTTGCATCAATTTATCAACGAGATGTGAACCAATG10.6369426751592357No Hit
CTACAATGCTTCTTTGACAAGAAGACTATCAGTGAAACTAAGAGACCTGTC10.6369426751592357No Hit
CTCCCACTGTGCACCGAATGCGGTGAGATTGGGAACCCCGGCGACCACAAC10.6369426751592357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGTTTT10.6369426751592357No Hit
GTGAACTGACCGTACCCCAAACCGACACAGGTGAACAAGTAGAGTATACTA10.6369426751592357No Hit
GTAACGAATTGATAGGTGAGAATCCTATCCGCCGGATGACTAAGGGTTCCT10.6369426751592357No Hit
GCGTATGATGTCGATGGCCCGAAGTAGAGCACTCAATGGGCTAGGGTGGCC10.6369426751592357No Hit
GGACCACATTTGAGCGTGGAAGAGGGCACCTGGGCAAAGGTACTATCACAT10.6369426751592357No Hit
GTACCAATTAACCTCCTCTTGAGTGTTCACACCTTCAATCTGGACGAGAGA10.6369426751592357No Hit
AGCCTTATGAGTGCCGGATTTGCCTAACACTCAGCCTTACTGCTTGGACGT10.6369426751592357No Hit
GGGTAATCTTGTGAAACTTTGTCGTGCTGGGGATAGAGCATTGCAATTATT10.6369426751592357No Hit
GTTCAAGTTCTGTGATTTGCAAGCCTGTTGTGAAACTGTCTCTTATACACA10.6369426751592357No Hit
CGTTGTACCTCTTTCCATCAGCCGGCTTCAAGAACTCATTAATGCTCAACG10.6369426751592357No Hit
AATCTAGGGTTTGTTGATCAGCCATGGATGATGACGATTGAAGGATGATCT10.6369426751592357No Hit
GCCATCTCCCGGGCCCCTTCCCCCACTTTTTTGGCTTTTCAAGACAGGGTT10.6369426751592357No Hit
GCCATGGAGCTAGACCAGTGAGCTATTACGCTTTCTTCAAAGGATGGCTGC10.6369426751592357No Hit
TTCTTCAATAGACTGGCGCAGGGTTCTGAAATTTCCAGTAGACTTTGACAT10.6369426751592357No Hit
CTTCAAAAGGCACGCCATCACACACACGGTGCTCTGACGGATTGTAAGCAC10.6369426751592357No Hit
GAGTTACAGAGTGTTGCTGATCTCAACAACCAAAGGTCGTTTCTTACTTTG10.6369426751592357No Hit
GAAGAGAAGTAAGCCGAGGGCGTCTTTGATTGTGTAGTAAGGGCTGTCTCT10.6369426751592357No Hit
GGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTA10.6369426751592357No Hit
TTCTAGCACTGATATCGTTGGTCGTATGCTTCTTTGTGTAAGAGAAAGATC10.6369426751592357No Hit
GCGTTGGAGGCCACATATTGCAGGATGGTGAGCCAGCCGTTCAGCACGCCG10.6369426751592357No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC10.6369426751592357RNA PCR Primer, Index 27 (95% over 21bp)
CAACAAAGAGTCATGATAACAGATAACGTAAGAGCTGTCTCTTATACACAT10.6369426751592357No Hit
ATTTATGATCTAGCAGGTCTACTTGTAAGTATACTCAAGAAATAGCTGTCT10.6369426751592357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAATACGTGGAACCCCAGAGT10.6369426751592357No Hit
GTCCTTCTCTAAGTGCAGCCTCATCAATCTGAATGACACCTGTCTCTTATA10.6369426751592357No Hit
GGTCGGCATCGATTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG10.6369426751592357No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACC10.6369426751592357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTATTTTTAAAATTC10.6369426751592357No Hit
CCTTTTTGCATGATGGGTCAGCGAGGAAATGGGAAGAGCGGCTTAAGCCAT10.6369426751592357No Hit
GGATATTAAGGACCAGAAGCTAGTTCTTAACATCTCTGCTGTCTCTTATAC10.6369426751592357No Hit
CGTCAGTAGTGGCGAGCGAACGCGGAAGAGGCCAAACCATGTGCATGTGAT10.6369426751592357No Hit
ATCCAATGGAAACTCAGCAGACCACGTGACCCAAAACACGTGACTCACGAT10.6369426751592357No Hit
AACTAAGGCGTTGGATTCCTCATTTGAAGCAGCAAAAACAACATTGGAACT10.6369426751592357No Hit
CTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTATGCGCTATCGGTCA10.6369426751592357No Hit
ACAATGGGTATTGATCTGATCGCCGGAGGTAAGAGTAAGAAGACCAAAAGG10.6369426751592357No Hit
GAACAGAGAAGTGTCCTACGGATTCAGAACCGGAAGGTCAGAAAAAAGTGG10.6369426751592357No Hit
ACCGTGAACACTGTCGTACTTGAACATGTAGGTCATGTACCTGTCTCTTAT10.6369426751592357No Hit
GCCTATTCTCGTTACAGAGCGCATTGATAAGAATAGAATAGGTACTGTCTC10.6369426751592357No Hit
GTTCTCGGATGGGTCTGCGTAAGAGCATATATGTTGGGACCCGAAAGATGG10.6369426751592357No Hit
CCTGGCTGTCTTCGCGATCCCACATCCTTTTCCACTTAGTACACCCTTAGG10.6369426751592357No Hit
CACCATGGGTTCCGATTTCACCACCTCCCTGCGCTGTCTCTTATACACATC10.6369426751592357No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6369426751592357No Hit
CCTTGATCTGTTTCCTTGATTTCTCAATCTTGGTGTCAAGTCCATTCCTGA10.6369426751592357No Hit
TCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATT10.6369426751592357No Hit
TAACGGTTCTATCGAATATGAAGATTGCTTTTAGGTTGAGTAATGCCCATT10.6369426751592357No Hit
GCTTAAATATGATAGAAACACAAGAGCAAGAACAAGTCTTATGCCTGTCTC10.6369426751592357No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers