FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_69.3410000000a3bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_69.3410000000a3bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021063
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC276871.369922659511356No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT33170.16412155385557006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31300.042.69841445
CGCTATA36150.037.3432931
ATGCCGT35800.037.26844844
GCCCACG36300.037.1889818
CCCACGA36650.036.89521819
GACGCTA36900.036.76720429
CGACGCT36950.036.71744528
ACGACGC36900.036.64525627
TATGCCG36900.036.2794143
CGTATGC36900.036.15746741
CGAGACG37600.035.96302823
ACGCTAT37950.035.74992830
TCGTATG37750.035.40292440
CGAGCCC38250.035.35189415
AGCCCAC38200.035.33926417
GTATGCC37900.035.26280642
TATCTCG37800.035.2370536
CCACGAG38400.035.213820
CCGAGCC38500.035.12233414
GCTATAT38550.035.0767832
Produced by FastQC (version 0.11.2)