##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23V3BCXX l02n01 kb2_2_63.3410000000a35f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.63256955810147	38.0	32.0	38.0	32.0	38.0
2	35.10229132569558	38.0	32.0	38.0	32.0	38.0
3	34.761047463175125	38.0	32.0	38.0	32.0	38.0
4	35.26759410801964	38.0	32.0	38.0	32.0	38.0
5	35.164484451718494	38.0	32.0	38.0	32.0	38.0
6	36.685761047463174	38.0	38.0	40.0	32.0	40.0
7	36.07937806873977	38.0	38.0	40.0	27.0	40.0
8	36.40589198036007	38.0	38.0	40.0	32.0	40.0
9	36.49918166939444	38.0	38.0	40.0	32.0	40.0
10	36.14975450081833	38.0	38.0	40.0	32.0	40.0
11	36.50163666121113	38.0	38.0	40.0	32.0	40.0
12	36.11292962356792	38.0	38.0	40.0	32.0	40.0
13	36.45417348608838	38.0	38.0	40.0	32.0	40.0
14	36.04664484451718	38.0	38.0	40.0	32.0	40.0
15	36.225859247135844	38.0	38.0	40.0	32.0	40.0
16	35.666939443535185	38.0	32.0	40.0	27.0	40.0
17	35.671849427168574	38.0	38.0	40.0	27.0	40.0
18	35.85024549918167	38.0	38.0	40.0	27.0	40.0
19	35.636661211129294	38.0	32.0	40.0	27.0	40.0
20	35.773322422258595	38.0	38.0	40.0	27.0	40.0
21	35.89770867430442	38.0	38.0	40.0	27.0	40.0
22	36.00327332242226	38.0	38.0	40.0	27.0	40.0
23	36.02045826513912	38.0	38.0	40.0	27.0	40.0
24	35.97626841243863	38.0	38.0	40.0	27.0	40.0
25	35.693126022913255	38.0	38.0	40.0	27.0	40.0
26	35.86415711947627	38.0	38.0	40.0	27.0	40.0
27	36.03846153846154	38.0	38.0	40.0	27.0	40.0
28	36.00081833060556	38.0	38.0	40.0	27.0	40.0
29	35.94844517184943	38.0	38.0	40.0	27.0	40.0
30	35.656301145662844	38.0	38.0	40.0	27.0	40.0
31	35.762684124386254	38.0	38.0	40.0	27.0	40.0
32	35.624386252045824	38.0	32.0	40.0	27.0	40.0
33	35.90998363338789	38.0	38.0	40.0	27.0	40.0
34	35.91080196399346	38.0	38.0	40.0	27.0	40.0
35	35.76759410801964	38.0	38.0	40.0	27.0	40.0
36	36.11374795417348	38.0	38.0	40.0	32.0	40.0
37	35.82242225859247	38.0	38.0	40.0	27.0	40.0
38	36.09083469721767	38.0	38.0	40.0	32.0	40.0
39	35.76923076923077	38.0	38.0	40.0	27.0	40.0
40	35.65384615384615	38.0	38.0	40.0	27.0	40.0
41	35.4860883797054	38.0	32.0	40.0	27.0	40.0
42	35.60229132569558	38.0	38.0	40.0	27.0	40.0
43	35.746317512274956	38.0	38.0	40.0	27.0	40.0
44	35.727495908346974	38.0	38.0	40.0	27.0	40.0
45	35.67757774140753	38.0	38.0	40.0	27.0	40.0
46	35.10392798690671	38.0	32.0	40.0	27.0	40.0
47	35.624386252045824	38.0	38.0	40.0	27.0	40.0
48	35.15875613747954	38.0	32.0	40.0	27.0	40.0
49	35.42471358428805	38.0	32.0	40.0	27.0	40.0
50	35.308510638297875	38.0	32.0	40.0	27.0	40.0
51	34.62684124386252	38.0	32.0	38.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	3.970899470899475
1101	2	2.3809523809523796
1101	3	3.2010582010582027
1101	4	2.1851851851851904
1101	5	-3.158730158730158
1101	6	0.8095238095238031
1101	7	-8.671957671957678
1101	8	-4.595238095238095
1101	9	-8.764550264550266
1101	10	-9.179894179894184
1101	11	-22.849206349206348
1101	12	-8.600529100529101
1101	13	-9.251322751322753
1101	14	-4.275132275132279
1101	15	-3.6455026455026456
1101	16	-22.243386243386247
1101	17	-22.957671957671963
1101	18	2.497354497354493
1101	19	2.857142857142854
1101	20	2.7010582010581956
1101	21	-3.169312169312157
1101	22	-22.486772486772495
1101	23	-9.208994708994716
1101	24	-8.801587301587297
1101	25	-3.6084656084656146
1101	26	1.669312169312164
1101	27	-21.814814814814817
1101	28	-22.402116402116405
1101	29	2.4312169312169303
1101	30	3.002645502645507
1101	31	-7.759259259259252
1101	32	-22.029100529100532
1101	33	-8.896825396825406
1101	34	-3.2989417989417973
1101	35	3.19047619047619
1101	36	1.272486772486772
1101	37	-22.817460317460316
1101	38	-22.70370370370371
1101	39	-2.859788359788361
1101	40	-22.441798941798936
1101	41	-7.526455026455025
1101	42	2.904761904761905
1101	43	-21.84391534391534
1101	44	2.5079365079365132
1101	45	-22.092592592592595
1101	46	3.338624338624335
1101	47	2.764550264550259
1101	48	-2.6005291005290943
1101	49	-21.753968253968253
1101	50	-22.082010582010582
1101	51	-7.30952380952381
2201	1	3.970899470899475
2201	2	2.3809523809523796
2201	3	-2.7989417989417973
2201	4	2.1851851851851904
2201	5	-3.158730158730158
2201	6	0.8095238095238031
2201	7	2.328042328042322
2201	8	1.404761904761905
2201	9	4.235449735449734
2201	10	3.820105820105816
2201	11	4.150793650793652
2201	12	2.3994708994708986
2201	13	1.7486772486772466
2201	14	3.724867724867721
2201	15	4.354497354497354
2201	16	2.7566137566137527
2201	17	2.0423280423280374
2201	18	2.497354497354493
2201	19	2.857142857142854
2201	20	2.7010582010581956
2201	21	4.830687830687843
2201	22	4.5132275132275055
2201	23	1.791005291005284
2201	24	2.198412698412703
2201	25	2.3915343915343854
2201	26	3.669312169312164
2201	27	5.185185185185183
2201	28	4.597883597883595
2201	29	2.4312169312169303
2201	30	5.002645502645507
2201	31	3.2407407407407476
2201	32	4.970899470899468
2201	33	4.103174603174594
2201	34	2.7010582010582027
2201	35	3.19047619047619
2201	36	1.272486772486772
2201	37	4.182539682539684
2201	38	4.2962962962962905
2201	39	5.140211640211639
2201	40	4.558201058201064
2201	41	5.473544973544975
2201	42	2.904761904761905
2201	43	3.1560846560846585
2201	44	2.5079365079365132
2201	45	2.9074074074074048
2201	46	5.338624338624335
2201	47	2.764550264550259
2201	48	5.399470899470906
2201	49	5.246031746031747
2201	50	2.9179894179894177
2201	51	3.69047619047619
2202	1	0.9708994708994751
2202	2	2.3809523809523796
2202	3	0.2010582010582027
2202	4	-0.8148148148148096
2202	5	-0.15873015873015817
2202	6	1.809523809523803
2202	7	2.328042328042322
2202	8	-1.595238095238095
2202	9	-3.264550264550266
2202	10	-1.179894179894184
2202	11	3.150793650793652
2202	12	-0.6005291005291014
2202	13	-0.2513227513227534
2202	14	2.724867724867721
2202	15	3.3544973544973544
2202	16	2.7566137566137527
2202	17	-0.9576719576719626
2202	18	-0.5026455026455068
2202	19	2.857142857142854
2202	20	2.7010582010581956
2202	21	-0.16931216931215687
2202	22	2.5132275132275055
2202	23	1.791005291005284
2202	24	3.198412698412703
2202	25	2.3915343915343854
2202	26	2.669312169312164
2202	27	0.18518518518518334
2202	28	-3.4021164021164054
2202	29	2.4312169312169303
2202	30	3.002645502645507
2202	31	3.2407407407407476
2202	32	3.970899470899468
2202	33	0.10317460317459393
2202	34	2.7010582010582027
2202	35	4.19047619047619
2202	36	-1.727513227513228
2202	37	2.1825396825396837
2202	38	2.2962962962962905
2202	39	-2.359788359788361
2202	40	0.5582010582010639
2202	41	-2.026455026455025
2202	42	-9.595238095238095
2202	43	-9.343915343915342
2202	44	-12.992063492063487
2202	45	2.9074074074074048
2202	46	3.338624338624335
2202	47	-0.23544973544974113
2202	48	4.399470899470906
2202	49	4.246031746031747
2202	50	2.9179894179894177
2202	51	0.6904761904761898
1102	1	3.970899470899475
1102	2	2.3809523809523796
1102	3	3.2010582010582027
1102	4	-3.8148148148148096
1102	5	-8.158730158730158
1102	6	-5.190476190476197
1102	7	2.328042328042322
1102	8	1.404761904761905
1102	9	-3.764550264550266
1102	10	1.820105820105816
1102	11	-22.849206349206348
1102	12	2.3994708994708986
1102	13	-4.251322751322753
1102	14	1.724867724867721
1102	15	2.3544973544973544
1102	16	2.7566137566137527
1102	17	2.0423280423280374
1102	18	2.497354497354493
1102	19	2.857142857142854
1102	20	2.7010582010581956
1102	21	-3.169312169312157
1102	22	2.5132275132275055
1102	23	1.791005291005284
1102	24	2.198412698412703
1102	25	2.3915343915343854
1102	26	1.669312169312164
1102	27	-2.8148148148148167
1102	28	2.5978835978835946
1102	29	2.4312169312169303
1102	30	3.002645502645507
1102	31	3.2407407407407476
1102	32	2.970899470899468
1102	33	2.103174603174594
1102	34	2.7010582010582027
1102	35	-2.80952380952381
1102	36	-9.727513227513228
1102	37	2.1825396825396837
1102	38	-8.70370370370371
1102	39	-2.859788359788361
1102	40	2.558201058201064
1102	41	3.473544973544975
1102	42	2.904761904761905
1102	43	3.1560846560846585
1102	44	2.5079365079365132
1102	45	2.9074074074074048
1102	46	5.338624338624335
1102	47	2.764550264550259
1102	48	3.3994708994709057
1102	49	5.246031746031747
1102	50	4.917989417989418
1102	51	3.69047619047619
2203	1	1.9708994708994751
2203	2	0.3809523809523796
2203	3	-0.7989417989417973
2203	4	2.1851851851851904
2203	5	0.8412698412698418
2203	6	1.4761904761904674
2203	7	2.328042328042322
2203	8	1.404761904761905
2203	9	2.235449735449734
2203	10	1.820105820105816
2203	11	2.8174603174603163
2203	12	2.3994708994708986
2203	13	2.415343915343911
2203	14	3.058201058201057
2203	15	3.0211640211640187
2203	16	2.7566137566137527
2203	17	3.375661375661373
2203	18	3.830687830687829
2203	19	4.19047619047619
2203	20	2.7010582010581956
2203	21	-1.1693121693121569
2203	22	2.5132275132275055
2203	23	-0.20899470899471595
2203	24	2.8650793650793673
2203	25	2.3915343915343854
2203	26	0.33597883597882827
2203	27	0.18518518518518334
2203	28	0.5978835978835946
2203	29	3.0978835978835946
2203	30	-5.330687830687825
2203	31	0.24074074074074758
2203	32	-4.6957671957672
2203	33	-0.8968253968254061
2203	34	3.367724867724867
2203	35	3.857142857142854
2203	36	2.605820105820108
2203	37	2.1825396825396837
2203	38	-0.7037037037037095
2203	39	-0.5264550264550252
2203	40	-1.441798941798936
2203	41	1.4735449735449748
2203	42	2.904761904761905
2203	43	3.1560846560846585
2203	44	2.5079365079365132
2203	45	3.574074074074069
2203	46	2.0052910052909994
2203	47	2.764550264550259
2203	48	3.3994708994709057
2203	49	-7.087301587301585
2203	50	-12.74867724867725
2203	51	-4.642857142857142
1103	1	-8.529100529100525
1103	2	-3.1190476190476204
1103	3	3.2010582010582027
1103	4	-0.8148148148148096
1103	5	-0.15873015873015817
1103	6	1.809523809523803
1103	7	0.32804232804232214
1103	8	-0.5952380952380949
1103	9	3.235449735449734
1103	10	-0.179894179894184
1103	11	3.150793650793652
1103	12	-2.1005291005291014
1103	13	2.7486772486772466
1103	14	-2.775132275132279
1103	15	-2.1455026455026456
1103	16	-1.7433862433862473
1103	17	0.04232804232803744
1103	18	3.497354497354493
1103	19	0.8571428571428541
1103	20	0.7010582010581956
1103	21	-1.6693121693121569
1103	22	0.5132275132275055
1103	23	2.791005291005284
1103	24	-9.301587301587297
1103	25	-2.1084656084656146
1103	26	-2.830687830687836
1103	27	-1.3148148148148167
1103	28	-1.9021164021164054
1103	29	0.4312169312169303
1103	30	-1.4973544973544932
1103	31	1.2407407407407476
1103	32	0.970899470899468
1103	33	0.10317460317459393
1103	34	3.7010582010582027
1103	35	3.19047619047619
1103	36	2.272486772486772
1103	37	-2.3174603174603163
1103	38	0.2962962962962905
1103	39	-1.3597883597883609
1103	40	3.558201058201064
1103	41	-1.0264550264550252
1103	42	3.904761904761905
1103	43	4.1560846560846585
1103	44	-1.9920634920634868
1103	45	3.9074074074074048
1103	46	4.338624338624335
1103	47	0.7645502645502589
1103	48	4.399470899470906
1103	49	1.246031746031747
1103	50	3.9179894179894177
1103	51	4.69047619047619
2204	1	3.970899470899475
2204	2	2.3809523809523796
2204	3	3.2010582010582027
2204	4	-3.8148148148148096
2204	5	2.841269841269842
2204	6	2.809523809523803
2204	7	4.328042328042322
2204	8	3.404761904761905
2204	9	4.235449735449734
2204	10	1.820105820105816
2204	11	2.150793650793652
2204	12	2.3994708994708986
2204	13	1.7486772486772466
2204	14	-4.275132275132279
2204	15	2.3544973544973544
2204	16	2.7566137566137527
2204	17	2.0423280423280374
2204	18	2.497354497354493
2204	19	4.857142857142854
2204	20	4.701058201058196
2204	21	4.830687830687843
2204	22	4.5132275132275055
2204	23	3.791005291005284
2204	24	2.198412698412703
2204	25	2.3915343915343854
2204	26	3.669312169312164
2204	27	5.185185185185183
2204	28	4.597883597883595
2204	29	4.43121693121693
2204	30	5.002645502645507
2204	31	3.2407407407407476
2204	32	4.970899470899468
2204	33	2.103174603174594
2204	34	2.7010582010582027
2204	35	-2.80952380952381
2204	36	1.272486772486772
2204	37	2.1825396825396837
2204	38	2.2962962962962905
2204	39	5.140211640211639
2204	40	-3.441798941798936
2204	41	3.473544973544975
2204	42	2.904761904761905
2204	43	3.1560846560846585
2204	44	2.5079365079365132
2204	45	2.9074074074074048
2204	46	3.338624338624335
2204	47	-3.235449735449741
2204	48	3.3994708994709057
2204	49	3.246031746031747
2204	50	2.9179894179894177
2204	51	-2.30952380952381
2205	1	-1.5291005291005249
2205	2	-0.6190476190476204
2205	3	-2.7989417989417973
2205	4	-0.8148148148148096
2205	5	-3.158730158730158
2205	6	0.8095238095238031
2205	7	2.328042328042322
2205	8	1.404761904761905
2205	9	2.235449735449734
2205	10	-4.179894179894184
2205	11	2.150793650793652
2205	12	-0.6005291005291014
2205	13	1.7486772486772466
2205	14	1.724867724867721
2205	15	-0.6455026455026456
2205	16	-1.7433862433862473
2205	17	0.04232804232803744
2205	18	-13.002645502645507
2205	19	-2.642857142857146
2205	20	-9.798941798941804
2205	21	-0.16931216931215687
2205	22	-8.986772486772495
2205	23	1.791005291005284
2205	24	-3.301587301587297
2205	25	-6.108465608465615
2205	26	-13.830687830687836
2205	27	-5.314814814814817
2205	28	-9.902116402116405
2205	29	-3.0687830687830697
2205	30	-2.997354497354493
2205	31	0.24074074074074758
2205	32	-3.029100529100532
2205	33	-3.396825396825406
2205	34	-9.798941798941797
2205	35	-2.30952380952381
2205	36	-4.227513227513228
2205	37	-0.8174603174603163
2205	38	2.2962962962962905
2205	39	-21.85978835978836
2205	40	2.558201058201064
2205	41	3.473544973544975
2205	42	-12.595238095238095
2205	43	-2.3439153439153415
2205	44	-9.992063492063487
2205	45	-2.5925925925925952
2205	46	-2.161375661375665
2205	47	2.764550264550259
2205	48	4.399470899470906
2205	49	0.24603174603174693
2205	50	2.9179894179894177
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1209	16	-4.910052910052915
1209	17	-0.9576719576719626
1209	18	-13.502645502645507
1209	19	-8.476190476190478
1209	20	-8.632275132275137
1209	21	1.4973544973545074
1209	22	0.5132275132275055
1209	23	-1.208994708994716
1209	24	2.8650793650793673
1209	25	0.3915343915343854
1209	26	2.3359788359788283
1209	27	0.18518518518518334
1209	28	1.2645502645502589
1209	29	3.0978835978835946
1209	30	1.669312169312171
1209	31	3.907407407407412
1209	32	3.6375661375661323
1209	33	-5.563492063492074
1209	34	-13.298941798941797
1209	35	-1.8095238095238102
1209	36	-0.06084656084656359
1209	37	-5.484126984126984
1209	38	0.9629629629629548
1209	39	-7.193121693121693
1209	40	3.224867724867728
1209	41	4.140211640211639
1209	42	4.238095238095241
1209	43	4.489417989417994
1209	44	3.1746031746031775
1209	45	4.2407407407407405
1209	46	4.005291005290999
1209	47	-4.235449735449741
1209	48	1.06613756613757
1209	49	-4.420634920634921
1209	50	-4.74867724867725
1209	51	2.357142857142854
1214	1	0.9708994708994751
1214	2	-0.6190476190476204
1214	3	0.2010582010582027
1214	4	2.1851851851851904
1214	5	-0.15873015873015817
1214	6	-3.690476190476197
1214	7	-9.171957671957678
1214	8	-3.095238095238095
1214	9	-2.264550264550266
1214	10	2.820105820105816
1214	11	-9.349206349206348
1214	12	-2.1005291005291014
1214	13	-2.7513227513227534
1214	14	-9.775132275132279
1214	15	-2.1455026455026456
1214	16	-8.743386243386247
1214	17	-2.4576719576719626
1214	18	3.497354497354493
1214	19	-8.642857142857146
1214	20	-8.798941798941804
1214	21	3.830687830687843
1214	22	-1.9867724867724945
1214	23	2.791005291005284
1214	24	3.198412698412703
1214	25	0.3915343915343854
1214	26	-2.830687830687836
1214	27	-8.314814814814817
1214	28	-1.9021164021164054
1214	29	-2.0687830687830697
1214	30	-8.497354497354493
1214	31	-8.259259259259252
1214	32	-8.529100529100532
1214	33	-2.396825396825406
1214	34	-8.798941798941797
1214	35	-1.3095238095238102
1214	36	2.272486772486772
1214	37	-9.317460317460316
1214	38	0.2962962962962905
1214	39	-1.3597883597883609
1214	40	3.558201058201064
1214	41	-8.026455026455025
1214	42	-1.595238095238095
1214	43	-1.3439153439153415
1214	44	0.5079365079365132
1214	45	3.9074074074074048
1214	46	-8.161375661375665
1214	47	-8.735449735449741
1214	48	-8.100529100529094
1214	49	-1.253968253968253
1214	50	-8.582010582010582
1214	51	3.69047619047619
1215	1	-1.5291005291005249
1215	2	-0.6190476190476204
1215	3	-9.298941798941797
1215	4	2.1851851851851904
1215	5	-0.15873015873015817
1215	6	0.8095238095238031
1215	7	-22.671957671957678
1215	8	1.404761904761905
1215	9	-3.764550264550266
1215	10	-10.679894179894184
1215	11	-2.349206349206348
1215	12	-0.6005291005291014
1215	13	-10.751322751322753
1215	14	-1.275132275132279
1215	15	-22.645502645502646
1215	16	-5.743386243386247
1215	17	-13.457671957671963
1215	18	-3.502645502645507
1215	19	2.857142857142854
1215	20	2.7010582010581956
1215	21	-9.669312169312157
1215	22	-5.9867724867724945
1215	23	-3.708994708994716
1215	24	-6.301587301587297
1215	25	-0.6084656084656146
1215	26	-10.830687830687836
1215	27	-2.3148148148148167
1215	28	-2.9021164021164054
1215	29	-10.06878306878307
1215	30	-7.997354497354493
1215	31	0.24074074074074758
1215	32	-9.529100529100532
1215	33	-10.396825396825406
1215	34	-15.298941798941797
1215	35	-12.30952380952381
1215	36	1.272486772486772
1215	37	-3.3174603174603163
1215	38	-0.7037037037037095
1215	39	-2.359788359788361
1215	40	2.558201058201064
1215	41	-9.026455026455025
1215	42	-12.595238095238095
1215	43	-14.843915343915342
1215	44	-5.992063492063487
1215	45	-0.09259259259259522
1215	46	3.338624338624335
1215	47	2.764550264550259
1215	48	1.3994708994709057
1215	49	-5.253968253968253
1215	50	-3.0820105820105823
1215	51	-8.80952380952381
1212	1	-4.529100529100525
1212	2	-10.11904761904762
1212	3	-12.298941798941797
1212	4	2.1851851851851904
1212	5	-9.658730158730158
1212	6	-10.690476190476197
1212	7	-3.171957671957678
1212	8	1.404761904761905
1212	9	2.235449735449734
1212	10	-0.179894179894184
1212	11	-3.349206349206348
1212	12	2.3994708994708986
1212	13	-0.2513227513227534
1212	14	1.724867724867721
1212	15	-0.6455026455026456
1212	16	-2.7433862433862473
1212	17	2.0423280423280374
1212	18	3.497354497354493
1212	19	-0.1428571428571459
1212	20	-1.7989417989418044
1212	21	3.830687830687843
1212	22	2.5132275132275055
1212	23	2.791005291005284
1212	24	2.198412698412703
1212	25	-0.6084656084656146
1212	26	-3.830687830687836
1212	27	-9.314814814814817
1212	28	-2.9021164021164054
1212	29	-3.0687830687830697
1212	30	-2.497354497354493
1212	31	-1.2592592592592524
1212	32	3.970899470899468
1212	33	-3.396825396825406
1212	34	-8.798941798941797
1212	35	-8.30952380952381
1212	36	2.272486772486772
1212	37	-2.3174603174603163
1212	38	-10.20370370370371
1212	39	-8.35978835978836
1212	40	-1.941798941798936
1212	41	-8.026455026455025
1212	42	3.904761904761905
1212	43	-9.343915343915342
1212	44	2.5079365079365132
1212	45	-9.592592592592595
1212	46	-9.161375661375665
1212	47	-2.735449735449741
1212	48	-2.1005291005290943
1212	49	4.246031746031747
1212	50	3.9179894179894177
1212	51	4.69047619047619
1213	1	0.9708994708994751
1213	2	-0.6190476190476204
1213	3	3.2010582010582027
1213	4	2.1851851851851904
1213	5	-0.15873015873015817
1213	6	0.8095238095238031
1213	7	2.328042328042322
1213	8	1.404761904761905
1213	9	3.235449735449734
1213	10	2.820105820105816
1213	11	3.150793650793652
1213	12	-0.6005291005291014
1213	13	1.7486772486772466
1213	14	1.724867724867721
1213	15	2.3544973544973544
1213	16	2.7566137566137527
1213	17	2.0423280423280374
1213	18	3.497354497354493
1213	19	-0.1428571428571459
1213	20	2.7010582010581956
1213	21	2.830687830687843
1213	22	3.5132275132275055
1213	23	2.791005291005284
1213	24	2.198412698412703
1213	25	3.3915343915343854
1213	26	1.669312169312164
1213	27	3.1851851851851833
1213	28	2.5978835978835946
1213	29	2.4312169312169303
1213	30	3.002645502645507
1213	31	3.2407407407407476
1213	32	2.970899470899468
1213	33	2.103174603174594
1213	34	2.7010582010582027
1213	35	3.19047619047619
1213	36	2.272486772486772
1213	37	-0.8174603174603163
1213	38	-0.7037037037037095
1213	39	4.140211640211639
1213	40	-0.4417989417989361
1213	41	0.4735449735449748
1213	42	2.904761904761905
1213	43	3.1560846560846585
1213	44	3.5079365079365132
1213	45	3.9074074074074048
1213	46	4.338624338624335
1213	47	3.764550264550259
1213	48	3.3994708994709057
1213	49	-2.753968253968253
1213	50	-0.08201058201058231
1213	51	0.6904761904761898
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	1.0
24	8.0
25	9.0
26	8.0
27	16.0
28	33.0
29	30.0
30	39.0
31	39.0
32	68.0
33	72.0
34	81.0
35	102.0
36	139.0
37	152.0
38	221.0
39	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.523731587561375	18.821603927986907	11.620294599018004	39.03436988543372
2	12.684124386252046	20.29459901800327	50.163666121112925	16.857610474631752
3	14.157119476268413	23.24058919803601	43.53518821603928	19.0671031096563
4	9.983633387888707	35.51554828150573	33.387888707037646	21.112929623567922
5	10.39279869067103	33.387888707037646	47.62684124386252	8.592471358428805
6	21.76759410801964	51.882160392798696	13.829787234042554	12.520458265139117
7	20.29459901800327	28.068739770867428	19.55810147299509	32.0785597381342
8	18.085106382978726	47.708674304418984	20.049099836333877	14.157119476268413
9	21.112929623567922	16.366612111292962	14.566284779050736	47.95417348608838
10	9.819967266775777	42.798690671031096	29.214402618657935	18.16693944353519
11	22.831423895253682	24.304418985270047	40.18003273322422	12.684124386252046
12	15.875613747954173	26.02291325695581	26.02291325695581	32.0785597381342
13	19.72176759410802	20.458265139116204	40.18003273322422	19.639934533551553
14	15.957446808510639	18.73977086743044	23.4860883797054	41.81669394435352
15	18.821603927986907	24.79541734860884	20.29459901800327	36.08837970540098
16	35.761047463175125	24.222585924713584	21.112929623567922	18.90343698854337
17	17.18494271685761	41.98036006546645	23.81342062193126	17.02127659574468
18	36.08837970540098	24.713584288052374	23.4860883797054	15.711947626841244
19	18.821603927986907	23.567921440261866	22.749590834697216	34.86088379705401
20	18.57610474631751	23.649754500818332	21.849427168576106	35.92471358428806
21	17.51227495908347	21.849427168576106	25.368248772504092	35.27004909983633
22	18.085106382978726	39.5253682487725	23.649754500818332	18.73977086743044
23	18.330605564648117	24.631751227495908	22.667757774140753	34.36988543371522
24	34.94271685761047	23.32242225859247	23.24058919803601	18.494271685761046
25	20.130932896890343	39.5253682487725	24.058919803600652	16.284779050736496
26	33.306055646481184	26.350245499181668	23.567921440261866	16.775777414075286
27	18.248772504091654	42.14402618657938	22.42225859247136	17.18494271685761
28	18.57610474631751	25.53191489361702	22.749590834697216	33.14238952536825
29	32.97872340425532	24.631751227495908	23.567921440261866	18.821603927986907
30	18.16693944353519	41.325695581014735	22.585924713584287	17.921440261865794
31	19.967266775777414	23.24058919803601	22.42225859247136	34.36988543371522
32	33.46972176759411	23.649754500818332	23.895253682487724	18.985270049099835
33	16.939443535188218	23.731587561374795	25.450081833060555	33.878887070376436
34	16.775777414075286	23.731587561374795	42.14402618657938	17.348608837970538
35	17.02127659574468	39.03436988543372	25.28641571194763	18.657937806873978
36	16.121112929623568	23.404255319148938	42.47135842880524	18.00327332242226
37	15.13911620294599	38.216039279869065	26.923076923076923	19.72176759410802
38	14.729950900163665	22.176759410801964	41.734860883797054	21.358428805237317
39	15.46644844517185	20.949263502454993	26.02291325695581	37.56137479541734
40	15.79378068739771	20.212765957446805	43.289689034369886	20.7037643207856
41	16.121112929623568	20.458265139116204	27.495908346972175	35.92471358428806
42	32.48772504091653	19.476268412438625	28.723404255319153	19.312602291325696
43	14.648117839607202	20.376432078559738	44.189852700491	20.78559738134206
44	14.238952536824879	35.84288052373159	27.905073649754502	22.013093289689035
45	15.13911620294599	20.78559738134206	44.762684124386254	19.312602291325696
46	31.58756137479542	20.130932896890343	27.00490998363339	21.27659574468085
47	12.684124386252046	20.130932896890343	30.27823240589198	36.90671031096563
48	12.274959083469723	19.55810147299509	27.414075286415713	40.752864157119475
49	28.150572831423897	19.803600654664486	30.687397708674304	21.358428805237317
50	12.356792144026187	20.376432078559738	44.59901800327332	22.667757774140753
51	12.111292962356792	20.376432078559738	30.032733224222586	37.47954173486088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	2.0
26	3.0
27	3.0
28	5.5
29	8.0
30	12.0
31	16.0
32	26.0
33	36.0
34	50.0
35	64.0
36	76.5
37	89.0
38	95.5
39	102.0
40	124.5
41	147.0
42	148.0
43	149.0
44	138.5
45	128.0
46	118.0
47	108.0
48	85.5
49	63.0
50	63.5
51	64.0
52	138.0
53	212.0
54	112.0
55	12.0
56	9.0
57	6.0
58	5.0
59	4.0
60	3.0
61	2.0
62	1.0
63	0.0
64	0.5
65	1.0
66	0.5
67	0.0
68	1.0
69	2.0
70	1.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1222.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.63338788870703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.11937377690802	82.89689034369886
2	0.5870841487279843	0.9819967266775778
3	0.09784735812133072	0.24549918166939444
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.09784735812133072	0.6546644844517185
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.09784735812133072	15.220949263502456
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	186	15.220949263502456	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT	8	0.6546644844517185	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG	3	0.24549918166939444	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT	2	0.16366612111292964	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGACGTC	2	0.16366612111292964	No Hit
CTTATACACATCCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT	2	0.16366612111292964	Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATATCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	2	0.16366612111292964	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATATCGTATGCCGTC	2	0.16366612111292964	No Hit
CTTATACACATCTCCAAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	2	0.16366612111292964	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.0
23	0.0	0.0	0.08183306055646482
24	0.0	0.0	0.24549918166939444
25	0.0	0.0	0.24549918166939444
26	0.0	0.0	0.24549918166939444
27	0.0	0.0	0.32733224222585927
28	0.0	0.0	0.40916530278232405
29	0.0	0.0	0.6546644844517185
30	0.0	0.0	0.9819967266775778
31	0.0	0.0	2.2913256955810146
32	0.0	0.0	3.2733224222585924
33	0.0	0.0	4.173486088379706
34	0.0	0.0	5.564648117839607
35	0.0	0.0	6.382978723404255
36	0.0	0.0	7.283142389525368
37	0.0	0.0	8.8379705400982
38	0.0	0.0	11.129296235679215
39	0.0	0.0	13.747954173486088
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAC	20	4.576611E-4	45.000004	17
GTATGCC	20	4.576611E-4	45.000004	42
CTATATC	20	4.576611E-4	45.000004	33
ACGCTAT	20	4.576611E-4	45.000004	30
ATCTCGT	20	4.576611E-4	45.000004	37
ATCTCCG	20	4.576611E-4	45.000004	10
CACATCT	20	4.576611E-4	45.000004	7
GCCCACG	20	4.576611E-4	45.000004	18
TATATCT	20	4.576611E-4	45.000004	34
TGCCGTC	20	4.576611E-4	45.000004	45
CCACGAG	20	4.576611E-4	45.000004	20
TATGCCG	20	4.576611E-4	45.000004	43
GAGCCCA	20	4.576611E-4	45.000004	16
CACGAGA	20	4.576611E-4	45.000004	21
CCCACGA	20	4.576611E-4	45.000004	19
CGCTATA	20	4.576611E-4	45.000004	31
ACATCTC	20	4.576611E-4	45.000004	8
GCTATAT	20	4.576611E-4	45.000004	32
TCTCGTA	20	4.576611E-4	45.000004	38
GACGCTA	20	4.576611E-4	45.000004	29
>>END_MODULE