FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_63.3410000000a35f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_63.3410000000a35f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC18615.220949263502456No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT80.6546644844517185No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG30.24549918166939444No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT20.16366612111292964No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGACGTC20.16366612111292964No Hit
CTTATACACATCCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT20.16366612111292964Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATATCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC20.16366612111292964No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATATCGTATGCCGTC20.16366612111292964No Hit
CTTATACACATCTCCAAGCCCACGAGACGACGCTATATCTCGTATGCCGTC20.16366612111292964No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC204.576611E-445.00000417
GTATGCC204.576611E-445.00000442
CTATATC204.576611E-445.00000433
ACGCTAT204.576611E-445.00000430
ATCTCGT204.576611E-445.00000437
ATCTCCG204.576611E-445.00000410
CACATCT204.576611E-445.0000047
GCCCACG204.576611E-445.00000418
TATATCT204.576611E-445.00000434
TGCCGTC204.576611E-445.00000445
CCACGAG204.576611E-445.00000420
TATGCCG204.576611E-445.00000443
GAGCCCA204.576611E-445.00000416
CACGAGA204.576611E-445.00000421
CCCACGA204.576611E-445.00000419
CGCTATA204.576611E-445.00000431
ACATCTC204.576611E-445.0000048
GCTATAT204.576611E-445.00000432
TCTCGTA204.576611E-445.00000438
GACGCTA204.576611E-445.00000429