FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_62.3410000000a342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_62.3410000000a342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC17513.39969372128637No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT120.9188361408882083No Hit
CTTATACACATCTCCGAGCCCCACGAGACGACGCTATATCTCGTATGCCGT30.22970903522205208No Hit
CTTATACACATCTCCGAGCCCACGAACGACGCTATATCTCGTATGCCGTCT20.1531393568147014RNA PCR Primer, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTT20.1531393568147014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC204.7411676E-445.017
GCCCACG204.7411676E-445.018
GAGCCCA204.7411676E-445.016
CCCACGA204.7411676E-445.019
CGAGCCC204.7411676E-445.015
ATCTCCG252.135184E-544.99999610
CATCTCC252.135184E-544.9999969
ACATCTC252.135184E-544.9999968
CCGAGCC252.135184E-544.99999614
TCTCCGA252.135184E-544.99999611
TCCGAGC252.135184E-544.99999613
CTCCGAG252.135184E-544.99999612
CACATCT306.282667E-537.4999967
TACACAT306.282667E-537.4999965
ATACACA306.282667E-537.4999964
GTATGCC250.001425380535.99999642
CTATATC250.001425380535.99999633
ACGCTAT250.001425380535.99999630
ATCTCGT250.001425380535.99999637
TATATCT250.001425380535.99999634
Produced by FastQC (version 0.11.2)