FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_59.3410000000a30e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_59.3410000000a30e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC15810.42904290429043No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT211.3861386138613863No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC90.594059405940594RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC70.46204620462046203TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC40.264026402640264No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG40.264026402640264No Hit
CTTATACACATCTCCGAGCCACGAGACTAGATCCTATCTCGTATGCCGTCT30.19801980198019803RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACTCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT30.19801980198019803No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT20.132013201320132No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG20.132013201320132No Hit
CTTATACACATCTCCGAGCCCACAGACTAGATCCTATCTCGTATGCCGTCT20.132013201320132RNA PCR Primer, Index 27 (95% over 21bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA20.132013201320132No Hit
CTTATACACATCTCCGAGCCCCACGAGACCTTGCAGAATCTCGTATGCCGT20.132013201320132No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC20.132013201320132No Hit
CTTATACACACTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.132013201320132RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC20.132013201320132No Hit
GTACAAGTACAGCTTGCTATCAGATCTCATTAATAGTCTAGCGGATTTGCC20.132013201320132No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers