FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_57.3410000000a2e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_57.3410000000a2e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC615.4708520179372195No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT110.9865470852017937No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC80.7174887892376681RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC50.4484304932735426No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG50.4484304932735426No Hit
CTTATACACTCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT30.26905829596412556No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20.17937219730941703No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG20.17937219730941703No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.17937219730941703RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG20.17937219730941703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17937219730941703No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC20.17937219730941703No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCCTGTCTCTTATACACA20.17937219730941703No Hit
GCTTCGTTCCAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATTCGATTTAT20.17937219730941703No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC150.00967432845.00000417
ATCCTAT150.00967432845.00000432
GTATGCC150.00967432845.00000442
ATCTCGT150.00967432845.00000437
CCTATCT150.00967432845.00000434
GCCCACG150.00967432845.00000418
TCCTATC150.00967432845.00000433
TGCCGTC150.00967432845.00000445
CCACGAG150.00967432845.00000420
TATGCCG150.00967432845.00000443
GAGCCCA150.00967432845.00000416
CACGAGA150.00967432845.00000421
CCCACGA150.00967432845.00000419
TCTCGTA150.00967432845.00000438
ACGAGAC150.00967432845.00000422
TATCTCG150.00967432845.00000436
GATCCTA150.00967432845.00000431
ATGCCGT150.00967432845.00000444
CGTATGC150.00967432845.00000441
CGAGCCC150.00967432845.00000415