FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_56.3410000000a2d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_56.3410000000a2d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC746.030969845150774No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT110.8964955175224123No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC80.6519967400162999No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC60.4889975550122249RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.4889975550122249TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG30.24449877750611246No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTG20.16299918500407498No Hit
CTTATACACACTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT20.16299918500407498RNA PCR Primer, Index 40 (95% over 21bp)
CTTCTATTCGAAGTGCTTTTCACCTTTCCTTCACAGTACTTGTTCACTATC20.16299918500407498No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC150.0099145644.99999617
ATCCTAT150.0099145644.99999632
GTATGCC150.0099145644.99999642
ATCTCGT150.0099145644.99999637
CCTATCT150.0099145644.99999634
ATCTCCG150.0099145644.99999610
CACATCT150.0099145644.9999967
GCCCACG150.0099145644.99999618
TCCTATC150.0099145644.99999633
TGCCGTC150.0099145644.99999645
CCACGAG150.0099145644.99999620
TATGCCG150.0099145644.99999643
CATCTCC150.0099145644.9999969
GAGCCCA150.0099145644.99999616
CACGAGA150.0099145644.99999621
CCCACGA150.0099145644.99999619
ACATCTC150.0099145644.9999968
TCTCGTA150.0099145644.99999638
ACGAGAC150.0099145644.99999622
TATCTCG150.0099145644.99999636
Produced by FastQC (version 0.11.2)