FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_55.3410000000a2c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_55.3410000000a2c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC12511.52073732718894No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT151.3824884792626728No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC111.0138248847926268RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC30.2764976958525346No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC30.2764976958525346TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT20.18433179723502305No Hit
ACTAGGGGAATCTCTATTGATGTCTTTTCCTCGGGTTACTGAGATGTTTCA20.18433179723502305No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT20.18433179723502305No Hit
CTTATACACATCTCCGAGCACACGAGACTAGATCCTATCTCGTATGCCGTC20.18433179723502305No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA20.18433179723502305No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG20.18433179723502305No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.18433179723502305No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC204.2812334E-445.042
ATCTCGT204.2812334E-445.037
TGCCGTC204.2812334E-445.045
TATGCCG204.2812334E-445.043
TACACAT204.2812334E-445.05
TCTCGTA204.2812334E-445.038
ACGAGAC204.2812334E-445.022
TATCTCG204.2812334E-445.036
ATGCCGT204.2812334E-445.044
CGTATGC204.2812334E-445.041
CCGAGCC204.2812334E-445.014
TCGTATG204.2812334E-445.040
TCCGAGC204.2812334E-445.013
CTCGTAT204.2812334E-445.039
CTATCTC204.2812334E-445.035
AGCCCAC150.00953857644.99999617
ATCTCCG150.00953857644.99999610
CACATCT150.00953857644.9999967
GCCCACG150.00953857644.99999618
CCACGAG150.00953857644.99999620
Produced by FastQC (version 0.11.2)