FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_52.3410000000a293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_52.3410000000a293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571755
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC393782.5053522972727937No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT46710.29718372138151306No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG18440.11732108375669237No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG16640.10586891722946642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC44200.043.6242445
ATGCCGT48850.039.47167644
GCCCACG50500.038.27111418
GTATGCC50600.038.15101242
CGAGACC50650.038.11335423
TATGCCG50700.038.07576843
CCCACGA50800.038.045119
CCACGAG50900.038.0145620
CTCGTAT50700.037.8538839
CGTATGC50950.037.8447841
CGAGCCC51500.037.79011515
ACGAGAC51450.037.6081922
ATCTCGT51300.037.58657537
CTTGCAG51200.037.57209829
CACGAGA51500.037.5279921
TCGTATG51450.037.52072540
CCGAGCC52000.037.51328314
CCTTGCA51300.037.45528
GACCTTG51650.037.3754426
AGCCCAC51800.037.35407617