FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_51.3410000000a286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_51.3410000000a286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590405
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC215471.3548121390463437No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT32400.20372169353089306No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG21180.13317362558593565No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG18100.1138074892873199No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA17850.112235562639705No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG15970.10041467424964082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC24700.042.5387945
ATGCCGT27900.037.6597944
TATGCCG29550.035.3285443
TATCTCG30000.034.72361836
CCCACGA30450.034.50601619
CCACGAG30500.034.44944820
CGTATGC30450.034.2104641
GTATGCC30700.034.15173342
TCGTATG30750.033.87669840
CTCGTAT30700.033.78529739
CGAGACT31050.033.6943123
AGACTAG31100.033.6401425
GCCCACG31300.033.5689518
ACGAGAC31350.033.5154122
GACTAGA31150.033.51391626
CCTATCT31550.033.01770434
GAGACTA31900.032.93755724
TCTCGTA31500.032.92725838
CACGAGA32100.032.7323421
GATCCTA32000.032.6940131