FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_50.3410000000a279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_50.3410000000a279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438852
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC41280.2868953860438739TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6050.038.676145
ATGCCGT6400.036.9125544
CCCACGA7000.035.35569419
CGTATGC6850.035.14454741
GTATGCC6900.034.88987742
TCGTATG6900.034.88987740
GCCCACG7100.034.22395318
TATGCCG7000.034.07003443
AGACTAC6600.033.74861525
CGAGACT7250.033.2055423
CACGAGA7600.031.97237621
CCACGAG7650.031.76340720
TCCGAGC7800.031.1525713
AGCCCAC7800.031.1525717
CCGAGCC7850.030.95414514
GAGCCCA8550.029.73562416
CTCGTAT8200.029.63293339
ACGAGAC8400.029.4630822
GCAATCT8350.027.48390634
CTCCGAG8900.027.30225412
Produced by FastQC (version 0.11.2)