FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_49.3410000000a250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_49.3410000000a250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743946
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.19840149688283826No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC13960.18764802821710178RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA12930.17380293730996604No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10640.14302113325429536No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9560.12850395055555108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.12635325682240378No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA8370.11250816591526804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.10538399292421763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG250.002107079435.9984421
TATGCCG1750.035.9984443
AATGCGG250.002107079435.9984431
CGAGACT352.8201434E-432.14146423
CACGAGA1900.031.972321
TATCTCG1900.031.972336
ATGCCGT2000.031.49863644
TGCCGTC2000.030.37368445
GTCCGTT300.00514719529.99870121
GACTAGC300.00514719529.99870119
ACGAGAC2200.029.65780422
TCGTACG1250.028.7987510
CGAGACG1900.028.41982323
TCTCGTA2400.028.12378338
GGCGTTA406.1656453E-428.12378111
CCCACGA2200.027.6124419
CCACGAG2200.027.6124420
GCCCACG2300.027.39011818
CGCCTAT856.4774213E-926.4694412
GAGACGA2050.026.34032424
Produced by FastQC (version 0.11.2)