FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_47.3410000000a236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_47.3410000000a236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC714.886441844459739No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT90.6194081211286993No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.41293874741913283TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.3441156228492774RNA PCR Primer, Index 27 (95% over 21bp)
GCATCTTACAGTGTCTTCGTTTTTTCTCTTTTGATTTATCAACGGAAGTCT30.20646937370956642No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC30.20646937370956642No Hit
CCTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAG20.13764624913971094No Hit
CTTATACACATCTCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT20.13764624913971094RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC204.950902E-445.017
ATCTCCG204.950902E-445.010
GCCCACG204.950902E-445.018
GAGCCCA204.950902E-445.016
CCCACGA204.950902E-445.019
CGAGCCC204.950902E-445.015
CCGAGCC204.950902E-445.014
CTAGATC204.950902E-445.028
TCTCCGA204.950902E-445.011
ACTAGAT204.950902E-445.027
TCCGAGC204.950902E-445.013
AGATCCT204.950902E-445.030
TAGATCC204.950902E-445.029
CTCCGAG204.950902E-445.012
ATCCTAT250.001487894236.032
GATCCTA250.001487894236.031
Produced by FastQC (version 0.11.2)