FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_45.3410000000a21c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_45.3410000000a21c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC322.756244616709733No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT60.516795865633075No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.4306632213608958RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC40.34453057708871665No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC40.34453057708871665TruSeq Adapter, Index 12 (95% over 21bp)
CTCCAGTAGCAGTAGAGAACGTTGAGGTACCAACAAAGACAGTGGAGGAGA20.17226528854435832No Hit
TCTTCTTTCAGTCGAGTTACTTCGTACCTTGACTGAAGATTCCTAAAGCTG20.17226528854435832No Hit
CTTATACACATCTCCGAGCCCATGAGACTAGATCCTATCTCGTATGCCGTC20.17226528854435832No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers