FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_43.3410000000a1f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_43.3410000000a1f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC736.500445235975066No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT151.335707925200356No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.44523597506678536RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTC30.26714158504007124TruSeq Adapter, Index 27 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG20.17809439002671415No Hit
GATTACGGTGGCGGCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTC20.17809439002671415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC150.00967432845.00000445
Produced by FastQC (version 0.11.2)