FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_41.3410000000a1d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_41.3410000000a1d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC716.2335381913959615No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT151.3169446883230904No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.4389815627743635RNA PCR Primer, Index 27 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT30.2633889376646181RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC30.2633889376646181TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG30.2633889376646181No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC20.17559262510974538No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTT20.17559262510974538No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)