FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_40.3410000000a1ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_40.3410000000a1ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC473.858784893267652No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT80.6568144499178982No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.41050903119868637RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC50.41050903119868637No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGG30.24630541871921183No Hit
GTCATAAGACAGGTATCGTAAATTTATTTACGAGGCAAACCGGGAGAAGTG20.16420361247947454No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.16420361247947454TruSeq Adapter, Index 12 (95% over 21bp)
ATAGTGAACAAGTACTGTGAAGGAAAGGTGAAAAGCACTTCGAATAGAAGG20.16420361247947454No Hit
GTATGGATCCATGGATCTATGTGTCTATATAGATCCTGTTCCTGGATTAAC20.16420361247947454No Hit
GTCTCACACTGTGCCAATCTACGAGGGTTTCTCTCTTCCTCATGCCATCCT20.16420361247947454No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)