FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_39.3410000000a1bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_39.3410000000a1bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001347
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC109820.5487304300553577No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT25310.1264648259397296No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCTGTCTCTT23820.11901984013766727No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT20820.10402993583821298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12600.041.42712445
ATGCCGT15550.033.56795544
CCCACGA16600.032.25790819
CCACGAG16450.032.14173520
GCCCACG16500.032.04433418
TATGCCG16550.031.6756343
ACGAGAC17000.031.36654922
CGTATGC16900.031.01962541
CACGAGA17150.030.82982421
GTATGCC17200.030.47858442
CGAGACT17450.030.42873423
TCGTATG17900.029.53807340
TATCTCG17800.029.19841636
CTCGTAT18050.029.16795539
CTAGATC18400.029.10224528
CGAGCCC18250.028.9715915
TCTCGTA18450.028.41364138
CCGAGCC18700.028.3947314
ACTAGAT19000.028.18322627
AGCCCAC18850.028.16877617
Produced by FastQC (version 0.11.2)