FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_38.3410000000a1a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_38.3410000000a1a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658886
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC22390.13497009438864394TruSeq Adapter, Index 12 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2950.038.89663745
TATGCCG3350.034.25226643
GCCCACG3400.033.7485518
CGAGCCC3500.032.7843115
CGTATGC3550.032.32255641
ATGCCGT3600.031.87363444
CCCACGA3800.030.19607419
AGCCCAC3900.029.99871417
CCGAGCC3900.029.99871414
GAGCCCA4250.028.05762316
CGAGACT4300.027.20813423
GTATGCC4350.026.3781842
CCACGAG4500.025.49890520
CTCCGAG4700.024.41384512
TCCGAGC4800.023.90522613
TCGTATG4900.023.41736440
CTCGTAT4950.023.18082639
CACGAGA5000.022.94901521
ACGAGAC5400.022.08238822
TCTCGTA5550.021.08017738
Produced by FastQC (version 0.11.2)