FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_37.3410000000a187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_37.3410000000a187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158728
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG2400.026.248699
GGTATCA6050.023.8539921
GTCGCGC2750.022.9079488
CGCGCGC2850.022.89359510
GCGCGCT4050.022.77664211
GGTCGCG2850.022.104167
GGGGCTA2900.020.1714136
ACGTTAG1351.9117579E-920.0439781
GCGCCAT3150.019.99900221
TCGGGCG1703.6379788E-1219.85195237
GCCCACG1151.5961996E-719.56424118
GTATCAA14250.019.1472741
GAGTCCG1656.002665E-1119.0899589
AGCGCCA3300.019.08995820
AGGTCGC3450.018.91216
GGCGGTA1951.8189894E-1218.46061740
TGCGCGC1608.1672624E-1018.28033810
CGTACAC1002.047536E-517.9991043
ATTATAG2001.8189894E-1217.9991043
GCGGGGG1507.2686817E-917.99910225
Produced by FastQC (version 0.11.2)