FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_3.34100000009f49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_3.34100000009f49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225189
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13150.5839539231489993No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA12080.5364382807330731No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10250.45517321005910594No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9930.4409629244767729No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.38056921075185735No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7330.3255043541203167No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7290.32372806842252505No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC7070.3139584970846711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.31218221138687946No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5790.25711735475533887No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.20072028385045448No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4100.18206928402364236No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.16030978422569486No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.135441784456612No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2850.12656035596765383No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2730.12123149887427893No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2590.11501449893200823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC800.036.56123742
GACTAGA800.036.56123726
TGACGTG250.002103876335.99875644
ATCTCGT850.034.41057637
CACGAGA850.034.41057621
TATCTCG850.034.41057636
TCGTATG850.034.41057640
CGTATGC900.032.49887541
GAGACTA900.032.49887524
TGCCGTC1050.032.14174745
AGATCCT950.030.78840830
CTCGTAT950.030.78840839
TATGCCG950.030.78840843
ACTAGAT950.030.78840827
GGTATAA300.005100479330.0456711
CTGACGT300.005139441729.99896643
CAATCAG300.005139441729.99896645
CTAGATC1001.8189894E-1229.2489928
GCCCACG1001.8189894E-1229.2489918
CCCACGA1001.8189894E-1229.2489919
Produced by FastQC (version 0.11.2)