FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_26.3410000000a0d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_26.3410000000a0d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455190
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20820.1430741002893093TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2900.027.92994718
TATGCCG3150.025.71328543
ATGCCGT3250.024.92210644
CCCACGA3500.023.78478819
TCGCGCG2300.023.4773489
CGTATGC3550.023.44979341
GTCGCGC2600.022.4991258
CGCGCGC2450.022.03995710
GTATGCC3800.021.31495942
TCGTATG4050.020.55475640
GGTCGCG2750.020.453757
GCGCGCG1109.9928E-820.4537499
CGCGCGA1109.9928E-820.45374910
GCGCGCT4250.019.58747111
CGCGCTT3000.019.4992412
CGTATAC1051.4466477E-619.2849643
GGTATCA8300.019.0084761
GGCGCAC600.00651277518.7492729
CTCGTAT4550.017.80150439
CTCCGAG4550.017.80150412
Produced by FastQC (version 0.11.2)