FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_25.3410000000a0be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_25.3410000000a0be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616175
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA14850.24100296182091124No Hit
ATCTAGCCATGAGCAGGTTGAAGAGAGCTCTAACAGGCCTTGGAGGACCGA12080.19604820059236416No Hit
CTCTAACGGCGTACCTTTTGCATGATGGGTCAGCGAGGAAATGGGAAGAGC11690.1897188298778756No Hit
TGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAGAACTAGAAGATTCCAC11380.1846877916176411No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA11090.1799813364709701No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10680.173327382642918No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAA10450.16959467683693755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.1644013470199213No Hit
CTTTAGGAATCTTCAGTCAAGGTACGAAGTAACTCGACTGAAAGAAGAGGG10050.16310301456566723No Hit
TTCCTAAAGGGCGCTGTGAACTGTAATTGTGAAAAGGTTGGAAGATCTGGC10010.16245384833854018No Hit
CTCCAGGTCTTGTCACCAAAAACCTTCCCCGATTGACAGAACGTTCCGCTA9670.1569359354079604No Hit
CCGTTAGAGTGAGTGCGCTTGACTACTCCTTCGACTGATTGTTCGCATCGG9590.15563760295370632No Hit
CTTTGGAACGAAGCTTCGTCTTTCTTTTTCTTAAAATCCCAAATCCGCTCT9090.1475230251146184No Hit
GGCTTAGAGGGTGGTCCCCCTTTCTCGCGTAAAAGCGATCAGAATTCGAAC8860.14379031930863798No Hit
TTCGTGTACCCATCGGACGGCAGCCCTTTCGGGGGTTCCTTAGGGACCGAT8690.14103136284334808No Hit
ACTATAAACAGACTCGCGACTATGGCAGGGCGGTACGCTCTGCTCTCTCGC8080.13113157787966082No Hit
CTCTTCAACCTGCTCATGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAG7820.1269119974033351No Hit
CTCCAAGAGAGAGGCATGGTTTTGTAGTCAAAGCAACTTCGTCACTTTCGT7400.12009575201850124No Hit
GTCTAGCTCCATGGCACCGAAAATGTATCAGGGCTCAAGTGATTCACCGAA7340.11912200267781069No Hit
CTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACAAGTACCGTGAGG7320.11879741956424716No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7190.11668762932608431No Hit
GACCTGGAGATATCAGAAGTGAGAATGCTGACATGAGTAACGATAAATCCT7180.11652533776930254No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG6910.11214346573619509No Hit
GCGTAGATTGACTGAACGCAGAAAGCCTTCCACTGGCAGGCGATCGTGTTT6870.11149429950906804No Hit
GTCCAAAGAGCGCAGACTAGCCACGGAGCTTGGATACGGTTTCCCGATCGG6860.11133200795228627No Hit
GTATTCGGAGTTTCCCTGGGGTTGGTAAGGCGAAATGGGGCCACCCTAGCC6840.11100742483872277No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC6640.10776159370308759RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAG6530.10597638657848826No Hit
GTCCCTAAGCAATCACTTAGTGGAAAAGGAAGTGATCGAGCGATGACAACC6450.1046780541242342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.10208138921572606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.10078305676147199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAG250.00210685535.998252
TCGTATG1100.032.72567740
CACGAGA1000.031.49846621
ATGCCGT1000.031.49846644
GACTTAT300.00509174330.0644131
CGTATGC1050.029.9985441
CTCGTAT1050.029.9985439
CCCACGA1050.029.9985419
GTATGCC1150.029.34639742
TATGCCG1150.027.3899743
CCACGAG1150.027.3899720
CGAGACG1004.5474735E-1126.99868623
ACTCTAT709.299747E-725.71303424
GACGAAT1058.367351E-1125.71303426
GACTCTA709.299747E-725.71303423
TCCGAGC1251.8189894E-1225.1987713
TATCTCG1101.5097612E-1024.54425836
ACGAATT1101.5097612E-1024.54425827
TATAGTA651.522835E-524.2295882
AGACTCT751.6962349E-623.9988322
Produced by FastQC (version 0.11.2)